Justyna Góraj, Elżbieta Węgrzynowicz-Lesiak and Marian Saniewski
In this study, we investigated the effect of plant growth regulators (PGRs) - auxins, gibberellin, cytokinin, abscisic acid, brassinosteroid, ethylene and their interaction with methyl jasmonate (JA-Me) applied to roots of the whole plants Kalanchoe blossfeldiana on the accumulation of anthocyanins in roots. The highest stimulation of anthocyanins synthesis was stated with application of JA-Me alone. In response to treatments with the other tested PGRs, the content of anthocyanins in roots of a whole plant was different depending on the concentration of the PGR when being applied alone or together with JA-Me. Auxin, indole-3-acetic acid (IAA) at a concentration of 50 mg·L-1, indole-3-butyric acid (IBA) at 5 mg·L-1 and abscisic acid (ABA) at 10 mg·L-1 induced anthocyanin accumulation with approximately 60-115% compared to the control while 24-epibrassinolid (epiBL), gibberellic acid (GA3) and 6-benzylaminopurine (BAP) had no effect on the anthocyanin accumulation. The simultaneous administration of the PGRs with JA-Me usually resulted in the accumulation of anthocyanins in roots in a manner similar to that caused by JA-Me. PGRs applied to isolated roots did not stimulate anthocyanin accumulation, except for the combination of JA-Me with 50 mg·L-1 IAA.
The results indicate that in K. blossfeldiana, the aboveground parts of the plant play an important role in the biosynthesis of anthocyanins in roots.
Emina Mladenović, Martina Zorić, Jelena Čukanović, Ksenija Hiel, Lazar Pavlović and Ivana Sentić
The purpose of this paper is to examine the effects of commercial plant growth regulators (PGRs), namely Inict-1 and Incit-5, on the rooting of African violet leaf cuttings (Saintpaulia ionantha H. Wendl.). The production of African violets has grown considerably in recent years, thus it is important to study the most rapid and cost-effective means of mass production. The propagated plant material of the cultivar ‘Nagano’ was treated with Incit-1 and Incit-5. The following parameters were observed: the occurrence of the first root, the number of formed secondary roots and root lengths, the occurrence of the first leaf, and the occurrence of the first flower. The results obtained showed that the use of commercial rooting hormones, namely Incit-1 and Incit-5, exerted positive effects on the duration of rooting, the number of secondary roots formed, and the root lengths of African violets. During the research, it was observed that the rooting durations of all the plants treated with Incit-1 and Incit-5 were 5 days shorter. The results indicate that the plants treated with PGRs necessitate less time to root, less time to produce new leaves, and, consequently, less time to produce flowers.
Maria Morozowska, Maria Wesołowska, Romana Głowicka-Wołoszyn and Anna Kosińska
Primula veris L. (Primulaceae) is a well-known medicinal herb. The callus induction response of three explant types: roots, cotyledons, and hypocotyls of four-week-old cowslip seedlings were evaluated. The highest statistically different callus induction rate was 93.6% and was obtained from root explants on MS medium supplemented with 0.1 mg/l BA and 5.0 mg/l PIC. Calli also appeared on 83.3% of cotyledon explants on MS medium supplemented with 1.0 mg/l BA and 3.5 mg/l 2,4-D and on 81.0% of root explants on MS medium supplemented with 0.1 mg/l KIN and 2.0 mg/l 2,4-D. These values were not statistically different. The average time required for callus initiation was 4 to 6 weeks, however, it depended on the explants type. The most suitable condition for callus proliferation and growth was MS medium with 0.5 mg/l TDZ and 0.1 mg/l NAA, and with 1.0 mg/l BA and 2.0 mg/l or 3.5 mg/l 2,4-D. No light conditions proved to be more favourable for cowslip calli induction and growth
., Yoshida K., 2010: Effects of spectral composition conversion film and plantgrowthregulators on proliferation of Cymbidium protocorm-like body (PLB) cultured in vitro. - Environmental Control in Biology, 48(3): 127-132.
Hasegawa A., 1991: Occurrence of variegation in the shoot of variegated Cymbidium multiplied by shoot tip culture. - Acta Horticulturae (ISHS), 300: 353-356.
Hossain M.M., kant r., van p.t., winarto b., Zeng S -J., teixeira da Silva J.A., 2013: The application of biotechnology to orchids. - Critical Reviews in Plant
.N., 2005. Combining priming and plantgrowthregulators improves muskmelon germination and emergence at low temperatures. Eur. J. Hortic. Sci. 70: 29-34.
Mangrich M.E., Saltveit M.E., 2000. Effect of chilling, heat shock, and vigour on the growth of cucumber (Cucumis sativus) radicles. Physiol. Plant. 109(2): 137-142.
Persoone G., Vangheluwe M.L., 2000. Toxicity determination of the sediments of the river Seine in France by application of a battery of microbiotests. In: New Microbiotests for Routine Toxicity Screening and Biomonitoring. G
Maciej Gąstoł, Iwona Domagała-Świątkiewicz and Michał Bijak
of ’Idared’ apple nursery trees. Hort. Sci. 29 (3-4): 46-51.
Hrotkó K., Magyar L., Yao C., Rónay Z., 1998. Effect of repeated BA (Benzyladnine) application on feathering of ‘Idared’ apple nursery trees. Acta Hort. 463: 169-176.
Hrotkó K., Magyar L., Rónay Z., 2000. Improved feathering on apple nursery trees by BA application. Acta Hort. 514(4): 113-119.
Jacyna T., 1996. Induction of lateral branching in nursery pear and apple trees with plantgrowthregulators. Fruit Varieties J. 50(3): 151-156.
potassium humate and ascorbic acid on growth, yield and chemical composition of common bean (Phaseolus vulgaris L.) grown under reclaimed soil conditions. - International Journal of Academic Research, 7(10): 192-199.
Barth C., De Tullio M., Conklin P.L., 2006: The role of ascorbic acid in the control of flowering time and the onset of senescence. - Journal of Experimental Botany, 57(8): 1657-1665.
Basra A.S., 2000: Plantgrowthregulators in agriculture and horticulture: their role and commercial uses. - Philadelphia.
florets) induced by plantgrowthregulators. Asia-Pacific J. Mol. Biol. Biotechnol. 22(1): 145-151.
Teixeira da Silva J.A., 2014c. Recent retraction cases in plant science that show why post-publication peer review is essential. J. Advancement Engin. Technol. 1(3): 4.
Teixeira da Silva J.A., Kim H.H., Engelmann F., 2014. Chrysanthemum low-temperature storage and cryopreservation: a review. Plant Cell, Tissue Organ Cult., DOI: 10.1007/s11240-014-0641-y.
Teixeira da Silva J.A., Shinoyama H., Aida R., Matsushita Y., Raj S
Ewa Dziadczyk, Marcin Domaciuk, Piotr Dziadczyk, Iwona Pawelec, Ewa Szczuka and Józef Bednara
The purpose of our investigation was to determine appropriate conditions for induction of raspberry (Rubus idaeus cv. Nawojka) cell suspension culture. The established callus culture obtained from leaf explants was used as an inoculum for cell culture initiation. Five combinations of plant growth regulators: 1) 4.0 mg l-1 IAA and 1.0 mg l-1 BAP; 2) 0.25 mg l-1 2,4-D; 3) 0.5 mg l-1 2,4-D; 4) 2.0 mg l-1 NAA and 2.0 mg l-1 BAP; 5) 4.0 mg l-1 NAA and 2.0 mg l-1 BAP, added into modified Murashige and Skoog (1962) medium, were tested in order to get the callus culture suitable for initiation of a cell suspension. The best callus (vigorously growing, healthy and friable) was obtained on the medium supplemented with 4.0 mg l-1 IAA and 1.0 mg l-1 BAP. To find the appropriate culture conditions for dispersing callus tissue in liquid medium into single cells and small aggregates, four combinations of plant hormones (auxins and cytokinins) were tested. The best culture medium for induction of raspberry cv. Nawojka cell suspension appeared to be the one supplemented with 1.0 mg l-1 2,4-D. Also the medium with 8.0 mg l-1 IAA and 1.0 mg l-1 BAP was similarly efficient.
Maliheh Fallahpour, Seied Mehdi Miri and Naser Bouzari
The purpose of the presented study was to determine the best mineral composition of media and plant growth regulators in the micropropagation of the ‘Gisela 5’ (Prunus cerasus × P. canescens) dwarf sweet cherry rootstock. Nodal explants from young healthy shoots were excised and cultured on Murashige and Skoog medium without growth regulators. In vitro raised shoot tips were transferred to three culture media including Murashige and Skoog (MS), Driver and Kuniyuki (DKW) and Lloyd and McCown (WPM) containing benzyl adenine (BA) (0.5, 1 or 2 mg·l−1) in combination with kinetin (Kin) at 0 or 0.5 mg·l−1. WPM and DKW media were proving to be the most effective, resulting in a higher percentage of shoot multiplication and shoot number as compared to MS. BA in concentration 2 mg·l−1 resulted in the highest number of microshoots per explant (3.1). For rooting, 0, 0.5, 1 or 2 mg·l−1 indole-3-butyric acid (IBA) on MS, DKW and WPM media were tested. WPM medium containing 2 mg·l−1 IBA was most effective for rooting (93.7%) in comparison to MS (53.1%) and DKW (14.0%). Rooted plantlets were successfully hardened and established in pots.