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The aim of the study was to determine the relations between vaginal culture results, elevated vaginal pH and abnormal vaginal microflora observed in microscopy, during the first trimester of pregnancy. A cross-sectional, observational study of 100 women receiving antenatal care in five outpatient clinics was carried out in Rîga from March 2010 until April 2011. Pregnant women at the first antenatal visit were submitted to a vaginal specimen collection for pH measurement, wet mount and cultures. Fifty pregnant women with vaginal pH 4.5 and 50 subsequent pregnant women with vaginal pH less than 4.5 were included. 96% of women with increased pH and 86% of women with normal vaginal pH showed positive cultures. Increased vaginal pH was significantly associated with M. hominis (P < 0.001), U. urealyticum (P = 0.017) and E. coli (P = 0.018). Abnormal vaginal microflora patterns showed similar associations with culture findings. Multivariate logistic regression analysis showed the highest risk of abnormal vaginal microflora associated with M. hominis (OR 14.4, 95% CI 1.6–124.4, P = 0.015) and E. coli (OR 8.5, 95% CI 1.6–45.9, P = 0.013). Increased vaginal pH and abnormal vaginal microflora pattern in wet mounts was associated with M. hominis and E. coli in vaginal cultures.
The experiment was conducted on 480 Hubbard Flex chickens (fast-growing) reared to 42 days of age and 480 Hubbard JA 957 chickens (slow-growing) reared to 63 day of age. Day-old chicks were randomly assigned to the three following groups according to the type of coccidiostat: C (control - no coccidiostat in the diet and birds not vaccinated against coccidiosis), A (plant coccidiostat adiCox® AP), and M (monensin coccidiostat). At the end of rearing period the results of the controlled production were presented, the chickens were slaughtered and samples of their intestines were collected for microflora composition analyses. The obtained results show that rearing time influenced the composition of enteric microflora (small intestine and blind gut). Moreover, a higher total count of bacteria was stated in intestinal digesta of the slow-growing chickens that were kept for three weeks longer than the Hubbard Flex chickens.
The study also proved a positive influence of the diet on the quantitative composition of enteric microflora. The lowest count of mesophilic bacteria and those from the Enterobacteriaceae family was observed in the chickens receiving adiCox® AP compared to the chickens of the control group and those receiving monensin.
belonging to the B2+D phylogenetic group in inflammatory bowel disease. Gut 2007; 56:669-675. 31. Walmsley RS, Anthony A, Sim R, Pounder RE, Wakefield AJ. Absence of Escherichia coli, Listeria monocytogenes, and Klebsiella pneumoniae antigens within inflammatory bowel disease tissues. J Clin Pathol. 1998; 51:657-661. 32. Araki Y, Mukaisho K, Sugihara H, Fujiyama Y, Hattori T. Proteus mirabilis sp. intestinal microflora grow in a dextran sulfate sodium-rich environment. Int J Mol Med. 2010; 25:203-208. 33. Videla S, Vilaseca J, Guarner F, Salas A, Treserra F, Crespo E
The aim of this work was to identify the main microbiota in raw cow milk from dairy farm of Slovakia and to describe the selected microorganisms responsible for thermostable protease and lipase production which can affected the quality of dairy products. The main bacterial classes identifying by MALDI-TOF MS were Gammaproteobacteria (62 %), Actinobacteria (19 %) and Bacilli (12 %). The dominant microbial genus of raw cow milk was Pseudomonas. From milk bacteria, the strain Lactococcus lactis and from the family Enterobacteriaceae, namely Enterococcus faecalis, Hafnia alvei, Citrobacter braakii and Raoultella ornithinolytica were observed in raw milk. The spoilage of milk products is caused by thermostable enzymes with lipolytic and proteolytic activity. Qualitative proteolytic and lipolytic activities were performed on skin milk agar and olive oil, respectively. From 16 identified microorganisms, only 8 strains (P. fragii, P. gessardii, P. lundesis, H. alvei, C. braakii, R. ornithinolytica, Kocuria rhizophila and Candida inconspicua) showed protease activity. Quantitative protease and lipase activities were determined by casein and olive oil, respectively. The highest both activities were measured for the genus Pseudomonas. While lipases produced by all isolated microbial species lose enzymatic activity at 77 °C for 30 – 40 min, almost proteases showed comparable activities during whole pasteurization experiment at selected experimental conditions (70 °C, 40 min).
adjuvant activity of a polydispersed beta-(1, 4)-linked acetylated mannan (acemannan). Vaccine, 10: 551–557. Choi S., Chung M.-H. (2003). A review on the relationship between Aloe vera components and their biologic effects. Semin. Integr. Med., 1: 53–62. Christaki E.V., Florou - Paneri P.C. (2010). Aloe vera: A plant for many uses. J. Food Agric. Environ., 8: 245–249. Dai B., Jiang L., Chen S. (2007). Effects of medicinal herb and polysaccharide from Aloe on gut microflora, immune function and growth performance in broiler. China Poultry., 29: 21–24. Darabighane B
arbuscular mycorrhizal fungi in a replant soil infested with root-knot nematodes. Mycorrhiza 10, 295-300. È atská V., 1994. Interrelationships between vesiculararbuscular mycorrhiza and rhizosphere microflora in apple replant disease. Biol. Plant. 36, 99-104. C heng F., C heng Z., 2016. Research progress on the use of plant allelopathy in agriculture and the physiological and ecological mechanisms of allelopathy. Front. Plant Sci. 6, 1020. C hon S.U., 2006. Alfalfa ( Medicago sativa L.) autotoxicity - replant problems. Korean J. Weed Sci. 26, 211-224. C omby M., M
emphasison the pig. Livest. Sci., 133: 10–19. Metzler B., Bauer E., Mosenthin R.. (2005). Microflora management in the gastrointestinal tract of piglets. Asian-Australas. J. Anim., 18: 1353–1362. Meulen Van Der J., Jansman A. (1997). Nitrogen metabolism in gastrointestinal tissue of the pig. Proc. Nutr. Soc., 56: 535–545. Muñoz R., Tor M., Estany J. (2012). Relationship between blood lipid indicators and fat content and composition in Duroc pigs. Livest. Sci., 148: 95–102. Namkung H., Li M., Gong J., Yu H., Cottrill M., De Lange C.F.M. (2004). Impact of feeding blends of