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Vladimír Repka, Roderik Fiala, Milada Čiamporová, Michal Martinka and Ján Pavlovkin
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HEDENFALK, I. – DUGGAN, D. – CHEN, Y. – RADMACHER, M. – BITTNER, M. SIMON, R. – MELTZER, P. – GUSTERSON, B. – ESTELLER, M. – KALLIONIEMI, P. – WILFORD, B. – BORG, A. – TRENT, J. 2001. Gene expression profiles in hereditary breast cancer
J-H. Kim, H. Lee, E-K. Bae, H. Shin, J-S. Lee, K-S. Kang and S-Y. Park
tent caterpillar (Malacosoma disstria): normalized and full-length cDNA libraries, expressed sequence tags, and a cDNA microarray for the study of insectinduced defences in poplar. Mol. Ecol. 15: 1275-1297.
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Baoquan Hu, Bin Wang, Chunguo Wang, Wenqin Song and Chengbin Chen
Triploidy is a widespread phenomenon in cultivated and natural breeding plants and it can confer some growth advantages. Here, we analyzed genome-wide gene expression in triploid Populus euramericana (black poplar) using the Affymetrix poplar microarray to detect any possible correlation between triploid vigor and a unique gene expression profile. Among the 38,400 transcripts that were detected in triploid poplar, 1,564 and 2,015 genes were up- or downregulated, respectively, compared with the diploid. The majority of the upregulated genes in the triploid were associated with carbon and nitrogen metabolism, especially lignin and secondary metabolism. Other genes upregulated in the triploid included genes involved in sugar transport, and brassinosteroid (BR) and auxin metabolism. Downregulated genes were mostly related to the assembly and biosynthesis of ribosomes and the nucleosome macromolecular complex. The results suggested that BR and auxin levels were crucial in controlling sugar transport, photosynthesis and cell wall biosynthesis. Downregulated genes were associated with chromatin regulation in the triploid. The information from this analysis could provide an insight into the vigor of triploid poplar.
aggressiveness of NFPAs [ 11 ]. Recently, CCNB1 was found to mediate the proliferation-inhibiting role of miR-410, a small non-coding RNA, in GnPA [ 12 ]. Additionally, Chesnokova et al . [ 13 ] have identified that human pituitary tumors originated from gonadotroph cells express abundant FOXL2 , and both FOXL2 and PTTG promote cluster- ing expression and secretion from gonadotroph cells, thus restraining the proliferation of pituitary cells.
Along with the development of microarray, transcriptome analysis has been widely utilized in understanding tumor mechanism
Aldona Kawęcka, Artur Gurgul and Anna Miksza-Cybulska
The conservation of farm animal genetic resources and their protection against genetic erosion requires knowledge of biodiversity status. Genetic variation in populations can be estimated using both traditional pedigree-based methods and molecular techniques. SNP microarrays are a new generation of molecular genetic tools, which have found application in analysis of biodiversity in populations of domestic and wild sheep, in studies of resistance to intestinal parasites and foot rot, and in searching for markers associated with meat and milk yield, or colour inheritance traits. The aim of the study is the review of recent literature on the biodiversity and the use of molecular markers for population genetics in different breeds and populations of sheep.
Katarzyna Stefańska, Agata Chamier-Gliszczyńska, Maurycy Jankowski, Piotr Celichowski, Magdalena Kulus, Magdalena Rojewska, Paweł Antosik, Dorota Bukowska, Małgorzata Bruska, Michał Nowicki, Bartosz Kempisty, Michal Jeseta and Jana Zakova
The correct oviductal development and morphogenesis of its epithelium are crucial factors influencing female fertility. Oviduct is involved in maintaining an optimal environment for gametes and preimplantation embryo development; secretory oviductal epithelial cells (OECs) synthesize components of oviductal fluid. Oviductal epithelium also participates in sperm binding and its hyperactivation. For better understanding of the genetic bases that underlay porcine oviductal development, OECs were isolated from porcine oviducts and established long-term primary culture. A microarray approach was utilized to determine the differentially expressed genes during specific time periods. Cells were harvested on day 7, 15 and 30 of in vitro primary culture and their RNA was isolated. Gene expression was analyzed and statistical analysis was performed. 48 differentially expressed genes belonging to “tube morphogenesis”, “tube development”, “morphogenesis of an epithelium”, “morphogenesis of branching structure” and “morphogenesis of branching epithelium” GO BP terms were selected, of which 10 most upregulated include BMP4, ARG1, SLIT2, FGFR1, DAB2, TNC, EPAS1, HHEX, ITGB3 and LOX. The results help to shed light on the porcine oviductal development and its epithelial morphogenesis, and show that after long-term culture the OECs still proliferate and maintain their tube forming properties.
Aerts, S., Lambrechts, D., Maity, S., Van Loo, P., Coessens, B., De Smet, F., Tranchevent, L. C., De Moor, B., Marynen, P., Hassan, B., Carmeliet, P., & Moreau, Y. (2006). Gene prioritization through genomic data fusion. Nat Biotechnol, 24, 537-544.
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