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Liquid Chromatography Metabolite Profiling of Tenofovir Disoproxil Fumarate
Reverse transcriptase inhibitors are the most frequently prescribed agents for HIV infections. This publication presents a validated, highly sensitive and selective isocratic HPLC method for the quantitative determination of tenofovir disoproxil fumarate and its metabolite tenofovir. Detection was performed on a UV detector. The linearity for the calibration curve in the concentration range of 80-20400 ng/mL for tenofovir disoproxil fumarate (TENDF) and 10-2560 ng/mL for tenofovir (TEN) is presented. Inter- and intra-day precision and accuracy of the proposed method were characterised by relative standard deviation (R.S.D.) and percentage deviation, respectively: with both lower than 4% for all analytes. The limit of detection was 33.46 ng/mL for TENDF and 2.10 ng/mL for TEN.
Afia Asif, Saed Khawaldeh, Muhammad Salman Khan and Ahmet Tekin
potential for screening sample contents. While in the second device, two straight channels serve as separate compartments for both drug and media, respectively with an additional cavity at the bottom for keeping the cells. These simplified miniaturized chips would serve as suspended microfluidics metabolites extraction platforms, and would be fabricated by making PDMS molds using soft lithography.
Materials and methods
The designs of the devices presented in this research work is inexpensive and simplified. The basic working principle of both the devices is
Dan Andonie, Zsolt Gáll, Paul Bosa, Maria Titica Dogaru and Szende Vancea
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Marta Piątkowska, Michael Sulyok, Katarzyna Pietruszka and Łukasz Panasiuk
Mycotoxins are low molecular weight secondary metabolites of fungi of different genera, e.g. Aspergillus, Alternaria, Fusarium and Penicillium . Mycotoxin production can occur under favourable conditions of humidity, temperature, and level of oxygen in the field, during harvesting, or post-harvesting during transport or storage. The presence of toxic metabolites in an animal diet may cause many serious health conditions such as teratogenesis, carcinogenesis, kidney and liver damage, or immune system suppression. The toxic effects differ by
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Stephen S. Hecht, Chi-Hong B. Chen, Ruth Young and Dietrich Hoffmann
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