Miodrag Dragoj, Jasna Bankovic, Ana Podolski-Renic, Sonja Stojkovic Buric, Milica Pesic, Nikola Tanic and Tijana Stankovic
’s lymphoma, medulloblastoma, neuroblastoma and lung cancer ( 5 ). In most studies on non-small cell lungcarcinoma (NSCLC), a predominant clinical form of lung cancer, c-Myc protein is overexpressed in 40–75% of cases ( 6 , 7 , 8 , 9 ). Even though it is highly expressed in NSCLC, it does not associate with histopathological parameters and its prognostic value for this type of lung cancer is not clear ( 7 , 9 , 10 ). These data implicate that c-Myc is important for NSCLC pathogenesis but not sufficient to individually drive this process. Accordingly, we evaluated
Sanja Mijatović, Milica Pešić, Marija Mojić, Jasna Banković, Đorde Miljković, Paolo Fagone, Katia Mangano, Ferdinando Nicoletti, James McCubrey, Nikola Tanić and Danijela Maksimović-Ivanić
saquinavir-NO against multidrug- resistant cancer cells. Neoplasia 2010; 12: 1023-30.
21. Pešić M, Marković JZ, Janković D, Kanazir S, Marković ID, Rakić L, et al. Induced resistance in the human non small cell lungcarcinoma (NCI-H460) cell line in vitro by anticancer drugs. J Chemother 2006; 18: 66-73.
22. Mijatović S, Maksimović-Ivanić D, Radović J, Miljković Dj, Harhaji Lj, Vučković O, et al. Anti-glioma action of aloe emodin: the role of ERK inhibition. Cell Mol Life Sci 2005; 62: 589-98.
23. Kaluđerović GN, Mijatović SA
Banu Yaman, Deniz Nart, Pervin Korkmaz Ekren, Gürsel Çok and Ali Veral
. Immunocytochemical expression of tissue specific transcription factor-1 in lungcarcinoma. J Clin Pathol. 1997;50:30-2.
7. Smith SL, Watson SG, Ratschiller D, Gugger M, Betticher DC, Heighway J. Maspin - the most commonly-expressed gene of the 18q21.3 serpin cluster in lung cancer - is strongly expressed in preneoplastic bronchial lesions. Oncogene. 2003;22:8677-87.
8. Katakura H, Takenaka K, Nakagawa M, Sonobe M, Adachi M, Ito S, Wada H, Tanaka F. Maspin gene expression is a significant prognostic factor in resected non-small cell lung cancer
Biljana Poposka, Snezana Smickova, Simonida Crvenkovska, Beti Ivanovska, Tome Stefanovski and Gordana Petrusevska
Silverberg E, Lubera JA. Cancer statistics. CA Cancer J Clin 1988; 38 : 5-22.
Evans WK. Rationale for the treatment of nonsmall cell lung cancer. Lung Cancer 1993; 9(Suppl 2) : S5-14.
Travis WD, Lubin J, Ries L, Devesa S. United States lungcarcinoma incidence trends: declining for the most histologic types among males, increasing among females. Cancer 1996; 77 : 2464-70.
Potti A, Willardson J, Forseen C, Ganti AK, Koch M, Hebert B, et
Wang Zhen-fei, Mu Yong-ping, Liang Jun-qing, Liu Yong-yan and Li Jing-quan
development of many human malignancies, including lungcarcinoma [ 11 ]. The overexpression of miR-21 in lung cancer cells promotes their migration, invasion, and chemoresistance by inhibiting the tumor suppressor PTEN or enhancing the activity of the Ras/MEK/ERK pathway [ 11 ].
PiRNA is a class of sncRNAs discovered in recent years, with a length of 25–31 nucleotides. They play important roles in regulating transposable element activities, chromatin integrity, DNA methylation, and mRNA stability [ 12 ] It has been found in multiple cancers, including lung cancer [ 13
Marina Markovic, Dalibor Jovanovic, Zeljko Todorovic, Marija Zivkovic, Aleksandar Dagovic, Slobodanka Mitrović, Marina Petrović and Jelena Nešić
7. Luh S, Kuo C, Tsao TC. Breast metastasis from small cell lungcarcinoma. Journal of Zhejiang University Science B. 2008;9(1):39-43. doi: 10.1631/jzus.B072258.
8. Assi HA, Khoury KE, Mouhieddine TH, Khalil LE, Kanj A, et al. Small Cell Lung Cancer with Metastasis to the Breast: A Case Report and Review of the Literature. J Cancer Biol Res.2014; 2(1):1025
9. Altintoprak F, Baytekin HF, Tasdemir C. Primary Small Cell Carcinoma of the Lung Presenting with Breast and Skin Metastases. The Korean Journal of Internal Medicine. 2011
B.A. Suciu, T. Mezei, Zsuzsanna Pap, L. Denes, Ioana Hălmaciu, Z. Pavai, Klara Brînzaniuc and L. Azamfirei
The aim of this study is to evaluate tumor angiogenesis in patients with non-small cell lung carcinoma. A total of 20 patients with pulmonary adenocarinoma have been included in the study. In order to evaluate tumor angiogenesis we studied the importance of CD34 expression. Evaluation of vascular density was performed with a semiautomated method using the dedicated software called ImageJ. We introduced in our study 20 patients with lung adenocarcinoma. We were able to identify tumor angiogenesis in 19 cases (95%). Immunolabeling of CD34 positive endothelial cells provided a good overview of tumor vascularization. Immunohistochemical staining of CD34 positive endothelium cells provided a good basis for tumor vascularity assessment, and also an excellent contrast for computer assisted morphometric measurements. Also we studied the intensity of the immunohistochemical staining of CD34 in the tumoral cells. We obtained the following results: a minor expression in 4 cases (20%), a moderate expression in 9 cases (45%) and an intense expression in 6 cases (30%). The histological type of adenocarcinomas influences the architecture and branching of the vessels. The density of newly developed vessels is higher in patients with papillary pulmonary adenocarcinomas, which may indicate a possible relationship between the histological type and development of vascular supply.
Žaklina Mijović, Zoran Pešić, Nikola Živković, Tijana Denčić, Ana Ristić Petrović and Slavica Stojnev
The sequence of precursor lesions for squamous cell carcinoma may be hyperplasia-metaplasia-dysplasia-carcinoma in situ. The aim of this study was to perform a karyometric analysis of squamous metaplasia, moderate dysplasia and squamous cell lung carcinoma. Bronchoscopic biopsies of normal mucosa in chronic bronchitis patients (n=10), squamous metaplasia (n=10), moderate dysplasia (n=11), squamous cell lung carcinoma (n=48), and normal appearing mucosa surrounding carcinoma (n=11) were retrieved. Three nuclear variables were estimated using an image analysis system. The mean equivalent diameter, nuclear area and volume of equivalent sphere of squamous cell lung carcinoma were significantly larger than in moderate dysplasia, squamous metaplasia and normal bronchial mucosa. Also, the values of equivalent diameter, nuclear area and volume of equivalent sphere were significantly larger in normal appearing mucosa surrounding carcinoma compared to normal mucosa in chronic bronchitis patients. Karyometric analysis may be a helpful ancillary tool in distinguishing squamous cell lung carcinoma from dysplasia, and dysplasia from squamous metaplasia in bronchoscopic biopsy specimens.
Deniz Köksal, Funda Demirağ, Hülya Bayiz, Adem Koyuncu, Neslihan Mutluay, Bahadır Berktaş and Mine Berkoğlu
Objective: Thoracentesis is the first investigation to be performed in a patient with lung cancer and pleural effusion. The diagnostic yield of conventional smear studies varies in the first thoracentesis. In this study, we aimed to investigate if the cell block method increases the diagnostic yield in exudative pleural effusions accompanying lung cancer.
Material and Method: Forty patients with lung cancer and exudative pleural effusions were included. Ten mililiters of fresh pleural fluid was obtained by thoracentesis from all patients in the initial evaluation. The pleural fluid sample was divided into two equal parts. One part was subjected to conventional smear and the other to the cell block method. Conventional smears were stained with May-Grünwald-Giemsa and Hematoxylin-Eosin. Cell block sections were stained with Hematoxylin-Eosin and mucicarmine. Conventional smear findings were grouped as “benign cytology” or “malignant cytology”. The cell block sections were evaluated for the presence of single tumor cells, acinary or papillary pattern, solid islands and staining with mucicarmine.
Results: There were 20 patients each in the benign and malignant conventional smear group. In the benign group, adding the cell block method to conventional smear provided a diagnosis of malignancy in 4 more patients and the diagnosis of malignant effusion was increased by a ratio of 10% (4/40). In the malignant group, adding the cell block technique provided the subtyping of lung cancer as adenocarcinoma in 7 patients (7/20, 35%).
Conclusion: Our study confirms that the cell block method combined with conventional smear increases the diagnostic yield in exudative pleural effusions accompanying lung cancer