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thereby ameliorated renal dysfunction and fibrosis. “IN VIVO CRYOTECHNIQUE” IN BIOMEDICAL RESEARCH AND APPLICATION FOR RENAL INJURY UNDER ACUTE HYPERTENSION It is well known that hemodynamic factors, such as blood flow and pressure, exert an important influence on kidney structure and function and the effect occurs almost split-second. For the routine immersion or perfusion fixation methods, animal specimens are inevitably exposed to stresses of ischemia and anoxia, exhibiting only dead morphological states of animal organ tissues without normal blood circulation

fluconazole and other azole antifungal drugs to murine and human granulocyte-macrophage progenitor cells in vitro. J Antimicrob Chemother 1999;43:675-81. doi: 10.1093/ jac/43.5.675 18. Feng L, Xia Y, Yoshimura T, Wilson CB. Modulation of neutrophil influx in glomerulonephritis in the rat with antimacrophage inflammatory protein-2 (MIP-2) antibody. J Clin Invest 1995;95:1009-17. doi: 10.1172/JCI117745 19. González-Flecha B, Cutrin JC, Boveris A. Time course and mechanism of oxidative stress and tissue damage in rat liver subjected to in vivo ischemia-reperfusion. Clin Invest

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.1111/j.1468-2494.2011.00673.x. 10. J. Y. Fang, C. F. Hung, T. L. Hwang and W. W. Wong, Transdermal delivery of tea catechins by electrical assisted methods, Skin Pharmacol. Physiol. 19 (2005) 28-37; DOI: 10.1159/000089141. 11. R. J. Batchelder, R. J. Calder, C. P. Thomasand and C. M. Heard, In vitro transdermal delivery of the major catechins and caffeine from extract of Camelia sinensis, Int. J. Pharm. 283 (2004) 45-51; DOI: 10.1016/ j.ijpharm.2004.06.007. 12. J. Y. Fang, C. F. Hung, T. L. Hwang and Y. L. Huang, Physicochemical characteristics and in vivo deposition

specimens of normal and cancerous tissues which showed that cancerous tissue can be as much as 7 times stiffer than normal tissue [ 14 ]. Besides, Fricke and Morse observed a higher capacitance in breast malignant tumors in comparison with the healthy breast [ 17 ]. Later in 1990, Morimoto et al studied the electrical impedance of breast tumors in-vivo and calculated the intracellular resistance, the extracellular resistance and cell membrane capacitance of normal and pathological tissue based on the impedance spectra at a frequency range of 0 to 300 kHz and a circuit

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Cancer; 1986, 123-159. 6. Butterworth BE: Consideration of both genotoxic and nongenotoxic mechanisms in predicting carcinogenic potential. Mutat Res 1990, 239:117–132. 7. Proctor DM, Gatto NM, Hong SJ, Allamneni KP: Mode-of-action framework for evaluating the relevance of rodent forestomach tumors in cancer risk assessment. Toxicol Sci 2007, 98:313-326. 8. King MT, Wild D, Gocke E, Eckhardt K: 5-Bromodeoxyuridine tablets with improved depot effect for analysis in vivo of sister-chromatid exchanges in bone-marrow and spermatogonial cells. Mutat Res 1982, 97

. Int. E. Engl. 37 (1998) 1328-1345; http://dx.doi.org/10.1002/(SICI)1521-3773(19980605)37:10<1328::AID-ANIE1328 7. A. N. El Meshad and M. I. Tadros, Transdermal delivery of an anti-cancer drug via w/o emulsions based on alkyl polyglucosides and lecithin: design, characterization, and in vivo evaluation of the possible irritation potential in rats, AAPS PharmSciTech. 12 (2011) 1-9; https://doi.org/10.1208/s12249-010-9557-y 8. A. Graf, E. Ablinger, S. Peters, A. Zimmer, S. Hook and T. Rades, Microemulsions containing lecithin and sugar-based surfactants: Nanoparticle