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Referances Abeydeera L.R. (2002). In vitro production of embryos in swine. Theriogenology, 57, 1: 256-273. Algriany O., Bevers M., Schoevers E., Colenbrander B., Dieleman S. (2004). Follicle size-dependent effects of sow follicular fluid on in vitro cumulus expansion, nuclear maturation and blastocyst formation of sow cumulus oocytes complexes. Theriogenology, 62, 8: 1483-1497. Anchorodoquy J.P., Anchorodoquy J.M., Picco S.J., Sirini M.A., Errecalde A.L., Furnus C.C. (2014). Influence of manganese on apoptosis and glutathione content of cumulus cells during

bacteria - Lett. Appl. Microbiol. 16: 150-153. Cebeci A., Gürakan C. 2003 - Properties of potential probiotic Lactobacillus plantarum strains - Food Microbiol. 20: 511-518. FAO 2002 - Guidelines for the Evaluation of Probiotics in Food - Report of a Joint FAO/WHO Working Group on Drafting Guidelines for the Evaluation of Probiotics in Food. London, Ontario, Kanada, April 30 and May 1. Geraylou Z., Vanhove M.P.M., Souffreau C., Rurangwa E., Buyse J., Ollevier F. 2014 - In vitro selection and characterization of putative probiotics isolated from the gut of Acipenser baerii

. Cell Biol. Morph., 3: 718-725 [4] Eisa S., H.W. Koyro, K.H. Kogel, J. Imani, 2005. Induction of somatic embryogenesis in cultured cells of Chenopodium quinoa, Plant Cell, Tissue and Organ Culture, Volume 81, Issue 2, pp 243-246 [5] Eeswara, J. P., Stuchbury, T., Allan, E., J. and Hordue, A. J. 1998. A Standard Procedure for the Micropropagation of the Neem Tree (Azadirachta indica A. Juss). Plant Cell Rep., 17: 215- 219. [6] Engelmann, F. 2004. Plant Cryopreservation: Progress and Prospects. In vitro Cell [7] Elif Burcu Bali, Leyla Açık, Pınar Elçi , Meral Sarper

-217. Koirala D., Pradhan S., Pant B. 2013. Asymbiotic seed germination and plantlet development of Coelogyne fuscescens Lindl., a medicinal orchid of Nepal. Scientific World 11(11): 97-100. DOI: 10.3126/sw.v11i11.8561 MOFSC 2013. Sunakhari Sankalan tatha kheti bikas (Karyabidhi) nirdesika 2069 BS, Kathmandu, Nepal. [in Nepali] Mukhopadhyay K., Roy S.C. 1994. In vitro induction of ‘runner’ - a quick method of micropropagation in orchid. Sci. Hortic. 56: 331-337. DOI: 10.1016/0304-4238(94)90051-5. Murashige T., Skoog F. 1962. A revised medium for rapid growth and bioassays

., 404: 330-335. Choi T. (2011). Dimethyl sulfoxide inhibits spontaneous oocyte fragmentation and delays inactivation of maturation promoting factor (MPF) during the prolonged culture of ovulated murine oocytes in vitro. Cytotechnology, 63: 279-284. Gitika B., Sai Ram M., Sharma S.K., Ilavazhagan G., Banerjee P.K. (2006). Quercetin protects C6 glial cells from oxidative stress induced by tertiary-butylhydroperoxide. Free Radic. Res., 40: 95-102. Ishige K., Schubert D., Sagara Y. (2001). Flavonoids protect neuronal cells from oxidative stress by three distinct

. Jensen T.K., Boesen H.T., Vigre H., Boye M.: Detection of Lawsonia intracellularis in formalin-fixed porcine intestinal tissue samples: comparison of immunofluorescence and in-situ hybridization, and evaluation of the effects of controlled autolysis. J Comp Pathol 2010, 142, 1-8. 10. Knittel P.J., Larson D.I., Harris D.L., Roof M.B., McOrist S.: United States isolates of Lawsonia intracellularis from porcine proliferative enteropathy resemble European isolates. Swine Health Prod 1996, 4, 119-122. 11. Koyama T., Hirai T., Nagai S.: In vitro cultivation and

References 1. Budna J, Celichowski P, Karimi P, Kranc W, Bryja A, Ciesiółka S, Rybska M, Borys S, Jeseta M, Bukowska D, Antosik P, Brüssow KP, Bruska M, Nowicki M, Zabel M, Kempisty B. Does Porcine oocytes maturation in vitro is regulated by genes involved in transforming growth factor beta receptor signaling pathway? Adv Cell Biol. 2017;5(1):1-14. 2. Ciesiółka S, Bryja A, Budna J, Kranc W, Chachuła A, Bukowska D, Piotrowska H, Porowski L, Antosik P, Bruska M, Brüssow KP, Nowicki M, Zabel M, Kempisty B. Epithelialization and stromalization of porcine follicular

References [1] Al-Edani T, Assou S, Ferrières A, Bringer Deutsch S, Gala A, Lecellier CH, Aït-Ahmed O, Hamamah S. Female aging alters expression of human cumulus cells genes that are essential for oocyte quality. Biomed Res Int. 2014; 2014: 964614. [2] Blaha M, Nemcova L, Kepkova KV, Vodicka P, Prochazka R. Gene expression analysis of pig cumulus- oocyte complexes stimulated in vitro with follicle stimulating hormone or epidermal growth factor-like peptides. Reprod Biol Endocrinol. 2015; 13: 113. [3] Brent A. Hanks, Alisha Holtzhausen, Katherine S. Evans, Rebekah

References Y. G. Bachhav and V. B. Patravale, Formulation and evaluation of meloxicam gels, Acta Pharm.   60 (2010) 153-163; DOI: 10.2478/v10007-010-0020-0. Y. G. Bachhav and V. B. Patravale, Microemulsion based vaginal gel of fluconazole: Formulation, in vitro and in vivo evaluation, Int. J Pharm.   365 (2009) 175-179; DOI: 10.1016/j.ijpharm.2008.08.021. C. M. Chakole, M. A. Shende and S. N. Khadatkar, Formulation and evaluation of novel combined halobetasol propionate and fusidic acid ointment, Int. J. Chem. Tech. Res.   1 (2009) 103-116. V. Rajni and

References Chang H.S., Chakrabarty D., Hahn E.J., Paek K.Y., 2003. Micropropagation of calla lily ( Zantedeschia. albomaculata ) via in vitro shoot tip proliferation. In vitro Cellular and Developmental Biology Plant 39, 2, 129-134. Ebrahim M.K.H., 2004. Comparison, determination and optimizing the conditions required for rhizome and shoot formation, and flowering of in vitro cultured calla explants. Sci. Hortic . 101 , 3, 305-313. Fan J.Q., Zhang W.W., Zhang N., Guo W.M., 2005. In vitro culture and rapid propagation of several varieties of