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12 Romanian Journal of Orthopaedic Surgery and Traumatology 2018; Special Issue 1 ROMSOS, SROA © 2018 THE PROGNOSTIC ROLE OF IMMUNOHISTOCHEMISTRY IN PRIMARY BONE TUMORS doi: 10.2478/rojost-2018-0023 Z. Pánti1,2, R. Ene1,2, M. Nica1,2, M. Popa1,2, M. Pleniceanu1, M. Cîrstoiu2,3, C. Cîrstoiu1,2 1Orthopedics and Traumatology Department, University Emergency Hospital, Bucharest, Romania 2“Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania 3Obstetrics and Gynecology Department, University Emergency Hospital, Bucharest, Romania In the last decades

incidence of esophageal adenocarcinoma in white males. Cancer. 2001;92:549-555. 22. Lagergren J, Lagergren P. Recent developments in esophageal adenocarcinoma. CA Cancer J Clin. 2013;63:232-248. 23. Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M. Applications of immunohistochemistry. J Pharm Bioallied Sci. 2012;4(Suppl 2):S307-9. 24. Le Beau MM. Fluorescence in situ hybridization in cancer diagnosis. Important Adv Oncol. 1993;29-45. 25. Hu L, Ru K, Zhang L, et al. Fluorescence in situ hybridization (FISH): an increasingly demanded tool for biomarker research and

JM, Jasani B, Miller K (2009) Chromogenic in situ hybridization: a multicenter study comparing silver in situ hybridization with FISH. Am J Clin Pathol 132:514–520. doi: 10.1309/ AJCPXY3MJ6GSRCYP [19] Ross JS (2011) Point: Fluorescence in situ hybridization is the preferred approach over immunohistochemistry for determining HER2 status. Clin Chem 57:980–982. doi: 10.1373/ clinchem.2010.160762 [20] Cuadros M, Villegas R (2009) Systematic review of HER2 breast cancer testing. Appl Immunohistochem Mol Morphol 17:1–7. doi: 10.1097/PAI.0b013e318169fc1c [21] Papadopoulos


Objective: Many epidemiological studies have shown that human papillomavirus related infections play a major role in cervical preinvasive and invasive lesions. ASCUS (atypical cells of undetermined significance) observed in about 4-5% of all cervical cytology specimens. We searched for the presence of HPV with immunohistochemical methods in the biopsy material of patients diagnosed with ASCUS using cytology.

Material and Method: The colposcopic biopsy or Loop Electro Excisional Procedure (LEEP) material of 115 patients with a diagnosis of ASCUS were evaluated. HPV (type 6,11,16,18,31,33,42,51,52,56 and 58) immunohistochemistry was applied to all materials. The relationship between the biopsy results and HPV positivity was investigated.

Results: Cervical intraepithelial neoplasia (CIN) I was found in 34.8%, CIN II in 13% and CIN III in 9.6% of the patients and cervicitis was present in 42.6% of the patients. HPV immunohistochemistry was positive in 11 cases (9.6%) and no staining was seen in 104 cases (90.4%). No positive staining was seen in the chronic cervicitis cases. The rate of positive staining was 15% (6/34) in the CIN I diagnosed group, 20% (3/12) in the CIN II diagnosed group and 18.2% (2/9) in the CIN III diagnosed group.

Conclusion: HPV positivity was found to be lower compared to the literature both in total and in cases diagnosed by biopsy. No staining occurred in any patient with cervicitis. In conclusion, we believe that immunohistochemical examination is not an appropriate method for the determination of HPV.

Maltese SF 10 N/ A (3.1kg) Perianal 28 Maltese IM 11 N/ A (4.6kg) Prepuce 29 Shih-Tzu IM 9 N/ A (10kg) Perianal 29 Mongrel CM 13 3 Perianal 30 Mongrel IM 10 N/ A Tail 30 Yorkshire Terrier IM 14 N/ A Perianal 31 Afghan Hound IM 9 3 Perianal 31 Yorkshire Terrier SF 13 N/ A Perianal 32 Dachshund IM 10 N/ A Unk 32 Shih-Tzu IM 15 N/A Perianal (8.9kg) 33 Beagle IM 10 3 Perianal Unk – unknown, IM – intact male, CM – castrated male, IF – intact female, SF – spayed female BCS – body condition score, BW – body weight Immunohistochemistry FFPE tissues were sectioned at 4 μm

distinguish basal cell carcinomas from trichoepitheliomas: a clinicopathologic and immunohistochemical study of 49 cases. Am J Dermatopathol 2010;32(7): 683-687. 11. Krahl D, Sellheyer K. p75 Neurotrophin receptor differentiates between morphoeic basal cell carcinoma and desmoplastic trichoepithelioma: insights into the histogenesis of adnexal tumours based on embryology and hair follicle biology. Br J Dermatol 2010;163(1):138-145. 12. Tebcherani AJ, de Andrade HF Jr, Sotto MN. Diagnostic utility of immunohistochemistry in distinguishing trichoepithelioma and basal cell

49 Romanian Journal of Orthopaedic Surgery and Traumatology 2018; Special Issue 1 ROMSOS, SROA © 2018 DIFFERENTIAL DIAGNOSIS OF BENIGN TUMORS OF BONE RICH IN GIANT CELLS, BASED ON IMMUNOHISTOCHEMISTRY doi: 10.2478/rojost-2018-0060 L. Ali1,3, D.M. Pop2,3,C.I. Stoica2,3 1”Victor Babeş” National Institute of Research and Development in the Biomedical Sciences and Biomedical Sciences, Bucharest, Romania 2“Foişor” Clinical Orthopaedic Hospital, Bucharest, Romania 3”Carol Davila” University of Medicine and Pharmacy, Bucharest, Romania Introduction. The aim of this


Background: Oral lichen planus (OLP) is a chronic immune-mediated inflammatory disease that usually causes oral mucosal damage. OLP has been considered to be a potentially malignant disorder associated with an increased risk for oral cancer. Trefoil factors (TFFs) are mainly synthesized by mucin-producing epithelial cells. Expression of TFFs in oral epithelia is thought to be an essential factor in protection against oral mucosal damage. However, very little is known about the connection between tissue damage of oral mucosa in OLP patients and expression of TFFs.

Objectives: To determine levels of TFF expression in oral mucosa from patients with OLP.

Methods: Tissue samples were collected from 35 patients with normal oral mucosa (controls) and 35 patients with OLP. An immunohistochemical method was used to determine the expression of the 3 TFFs (TFF1, 2, and 3) in mucosal tissues of the oral cavity.

Results:TFF2 and TFF3 expression in oral epithelia were significantly decreased in OLP patients (Mann- Whitney U test; P = 0.006, and P = 0.002, respectively). There were no significant differences in TFF1 expression between OLP patients and control subjects.

Conclusion: The present study demonstrated reduced expression of TFF2 and TFF3 in oral epithelia of OLP patients. These outcomes support our previous observations that chronic inflammation may play a major role in downregulation of TFF expression, which may be associated with oral mucosal damage in OLP patients.


The research carried out at a mink farm aimed to determine the effect of blood plasma supplemented diet applied at the period preparing mink for reproduction on the animal organism. The studies included four groups of mink. The control group received a non-supplemented diet, while the experimental groups had feed with additive of 0.5%, 1.5%, and 2.5% of beef-pork plasma in the daily feed ration. The pathomorphological and immunohistochemical evaluation was performed on the liver, kidneys, lymph nodes, spleen, and bowel from all the groups. Pathomorphological and immunohistochemical changes of various intensity were observed in the examined organs from all experimental groups.

humidity). The HG (G20, P1, and P3) experimental animals and their respective stationary controls were humanely sacrificed using isoflurane gas. Abdominal mammary gland tissue was then collected as described previously ( Patel et al., 2008 ). The animals from SC and HG groups were sacrificed at the same time in the circadian cycle. During the experimental period, the centrifuge was paused on a daily basis for about an hour to clean cages and replenish food and water, as well as to monitor animal behavior and overall health. Immunohistochemistry The tissue for