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, WI. [13] Huang, Y. (2004), Evaluation of genetic diversity in sorghum germplasm using molecular markers. International Plant & Animal Genome XII Conference , San Diego, CA, Poster 265. p.138. [14] Dahlberg, J. A., Zhag, X., Hart, G. E., Mullet, J. E. (2002), Comparative assessment of variation among sorghum germplasm accessions using seed morphology and RAPD measurements. Crop Science Journal 42(1), 291–296. [15] Jayarama Chandran, M., Kumaravadivel, N., Kandasamy, G., Eapen, S. (2011), Comparison of genetic variability induced by radiation and tissue culture in


The threatened European species Arnica montana L. is a plant much appreciated in the pharmaceutical industry, Galicia (North-Western Spain) being one of the main supply regions. Two chemotypes based on the content of sesquiterpene lactones have been found in this area: a chemotype dominated by helenalins, common in Central Europe, and another dominated by dihydrohelenalins, only found currently in Galicia. The aim of this work was to analyse the phylogenetic relationships and genetic diversity of Arnica montana in Galicia and their concordance with previous biochemical and environmental information. Two polymorphic chloroplast markers were sequenced in individuals from populations showing significant biochemical differences and situated in different environments (heathland, hay-meadow and peatland). Three haplotypes, different from those previously described in Europe, were found in Galicia and our results suggest that one of them could be ancestral inside the species. Significant population differentiation was detected in the studied area but genetic diversity within populations was low, only showing variability in heathland populations. This study is the first one describing the genetic diversity of Arnica montana in the extreme SW of Europe. The results suggested the presence of two different genetic groups (one of them ancestral) and were congruent with the two chemotypes described. Plants from heathlands displayed the highest variability because the two chemotypes were present in them. Future design of conservation and economical management plans for this threatened species should take into account this genetic variability and prioritize further genetic and chemical characterizations across the distribution range of the species.

–27. Boichard D., Maignel L., Verrier É. (1997). The value of using probabilities of gene origin to measure genetic variability in a population. Genet. Sel. Evol., 29: 29–23. Bokor Á., Jónás D., Ducro B., Nagy I., Bokor J., Szabari M. (2013). Pedigree analysis of the Hungarian Thoroughbred population. Livest. Sci., 151: 1–10. Cervantes I., Molina A., Goyache F., Gutiérrez J.P., Valera M. (2008). Population history and genetic variability in the Spanish Arab Horse assessed via pedigree analysis. Livest. Sci., 113: 24–33. Egerszegi I., Rátky J., Solti L., Brüssow K.P. (2003

Journal of Tropical Biomedicine 1(2): 133–138. DOI: 10.1016/S2221-1691(11)60011-7. Gonor K.V., Pogozheva A.V., Derbeneva S.A., Mal'tsev G., Trushina E.N., Mustafina O.K. 2006. The influence of a diet with including amaranth oil on anti-oxidant and immune status in patients with ischemic heart disease and hyperlipoproteidemia. Voprosy Pitaniia 75(6): 30–33. [in Russian] Grubben G.J.H., Denton O.A. 2004. Plant Resources of Tropical Africa 2: Vegetables. PROTA, Wageningen, Netherlands, 668 p. Hamdi A., El-Ghareib A.A., Shafey S.A., Ibrahim M.A.M. 2003. Genetic variability


The genus Acidithiobacillus comprises 7 species of Gram-negative obligatory acidophilic chemolithotrophic bacteria that derive energy mainly from the oxidation of reduced sulphur compounds. Four of the species also catalyse the dissimilatory oxidation of ferrous iron while three (A. thiooxidans, A. albertensis, and A. caldus) do not. Bacteria from the genus Acidithiobacillus are often associated with mineral biotechnologies (biomining) and acid mine drainage. While acceleration of mineral solubilisation is a positive aspect in environmental biotechnologies, it is undesirable in acid mine drainage with strong negative ecological impact and there is profound interest in genetics and genomics of these bacteria. Representatives of Acidithiobacillus genus occur world-wide, however there are limited data on Acidithiobacillus spp. variability from Slovakia. In our work the variability of Acidithiobacillus spp., from Slovakia was analysed and the presence of A ferrooxidans was detected. In addition, for the first time we report here on the occurrence of A. albertensis as well. Comparative analyses confirmed pronounced genetic and genomic diversity within the genus, especially within A. ferrooxidans and A. thioxidans complexes. Based on data presented, several Acidithiobacillus species could be considered as a complex species and the description of several new species is very probable in the near future.


To date it is unknown if there is a predisposition to sepsis. In this respect, genetic studies have been conducted with the aim to find gene variants which can point out a higher predisposition to developing sepsis. The primary objective of this study is to highlight whether the genetic polymorphism of Angiopoietin-2 gene (ANG2-35G>C) is present mainly in septic patients. As secondary objectives we aimed to evaluate if there are any associations between ANG2-35G>C polymorphism and the severity scores Acute Physiology and Chronic Health Evaluation II (APACHE II) and Simplified Acute Physiology Score (SAPS) as well as routine tests in septic patients such as C reactive protein (CRP), procalcitonin (PCT). We enrolled adult patients admitted to the Intensive Care Unit (ICU). After admission to the ICU and the diagnosis of sepsis, blood samples were collected and the severity scores: APACHE II, SAPS were calculated on the first day of ICU admission. We recorded the following from the blood samples: CRP, PCT, angiopoietine2 (Ang-2). We performed several one-way ANOVA tests to determine any significant mean difference of the analyzed variables. We observed that variant genotypes of ANG2-35G>C gene polymorphism are significantly related to CRP, aspect which increases this biomarker credibility compared with others (i.e., PCT), in septic patients. ANG2-35G>C gene polymorphism is associated with severity scores, APACHE II, and SAPS in sepsis.

: Wheat breeding in abiotic stress conditions of solonetz. Genetika, Vol 44, No. 1, pp. 91 – 100, 2012. DIMITRIJEVIĆ M, PETROVIĆ S, BANJAC B, BARAĆ G, YURIEVNA DRAGOVIĆ A., MIHAILOVICH KUDRYAVTSEV A., KNEŽEVIĆ D: Genetic variability of the cereal ( Poaceae ) germplasm collection monitored by protein and molecular markers. AGROFOR International Journal, Volume 2. Issue No. 1. pp. 75-82, 2017. DONALD CM: In search of yield. Austr. J. Agric. Res., 28, 171-178, 1962. FAO: Building on Gender, Agrobiodiversity and Local Knowledge. A Training Manual, 2005. http

The reasons for low intrapopulation genetic variation in Lamium incisum Willd

The paper presents results of a study which aim was preliminary screening of intrapopulation genetic variability in Lamium incisum Willd. This weed species is rarely distributed in Poland and lessening its count during the last years. As a plant inhabiting anthropogenic sites it is exposed to extreme conditions and disturbances caused mostly by the progressive intensification of agriculture. In order to investigate the genetic variability of the selected population markers of ISSR category were used. The analysis of chosen individuals with use of three ISSR primers revealed total of 49 loci, of which only 15 were polymorphic. Nei's gene diversity index (HE=0.099) and the mean number of alleles per locus (AE=1.160) indicated low genetic diversity within the examined population. The research presented in this paper allows for a better learning of the genetic variability of the investigated species and considers probable factors influencing its level.

characters. Plant Genetic Resources Newsletter 128: 64-67. AWOPEU, J. A. (1982): Genetic variability studies in Lupinus albus L. Ph. D Thesis, University of Reading, U. K. AWOPETU, J. A. and O. M. ALIYU (2000): Genetic Diversity in Vigna unguiculata L. Walp. (Fabaceae). Moor Journal of Agricultural Research 1 (1): 22-31. BEHRENS, R. (1998): About the spacing of cashew nut trees. Proceedings of International Cashew and Coconut Conference, 17-21 February 1997, Dar es Salaam, Tanzania. BioHybrid International Limited, U.K., pp. 48-52. CASAS, A. M., E. LGARTUA, G. BALAGUER and

triggers DNA repair mechanisms, however, their role in the development of MM has not been fully studied yet. 12 , 13 It has been suggested that the genetic variability of proteins involved in DNA repair mechanisms affects the risk of MM. In particular, excision repair cross-complementing group 1 (ERCC1) and X-ray repair cross-complementing protein 1 (XRCC1) may be involved and genes coding for these proteins are known to be polymorphic. 14 , 15 ERCC1 is a protein involved in the repair of DNA by nucleotide excision repair (NER). Together with the Xeroderma pigmentosum F