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classical swine fever virus by restriction fragment length polymorphism of the E2 gene. J Virol Methods 2000, 87, 145-149. 20. Paton D.J, Sands J.J, Lowings J.P., Smith J.E, Ibata G, Edwards S.: A proposed division of the pestivirus genus using monoclonal antibodies, supported by cross-neutralisation assays and genetic sequencing. Vet Res 1995, 26, 92-109. 21. Plowright W.: Other virus diseases in relation to the J.P. 15 programme. In: Joint Campaign Against Rinderpest. First Technical Review Meeting, Phase IV. Mogadiscio, Kenya, 1969, pp. 19-23. 22. Rola M, Polak M

Biogeography 12: 293-314. SYED, N. H., M. J. SURESHSUNDAR, B. S. Bhau, J. J. V. CAVALCANTI and A. J. FLAVELL (2005): Ty1-copia retrotransposon- based SSAP marker development in cashew (Anacardium occidentale L.), Theoretical and Applied Genetics 110: 1195-1202. VEKEMANS, X., T. BEAUWENS, M. LEMAIRE and I. ROLDANRUIZ (2002): Data from amplified fragment polymorphism (AFLP) markers show indication of size homoplasy and of a relationship between degree of homoplasy and fragment size, Molecular Ecology 11: 139-151. VENDRAMIN, G. G., M. ANZIDEI, A. MADAGHIELE and G. BUCCI (1998

floodplains. Freshwater Biol 47(4):733-748 https://doi.org/10.1046/j.1365-2427.2002.00894.x Kikuchi S, W Suzuki and N Sashimura (2011) Gene flow in an endangered willow Salix hukaoana (Salicaceae) in natural and fragmented riparian landscapes. Conserv Genet 12(1):79-89 https://doi.org/10.1007/s10592-009-9992-z Klimo E and H Hager (eds) (2001) The floodplain forests in Europe: current situa­tion and perspectives. Leiden, The Netherlands: Koninklijke Brill NV Lewis PO and D Zaykin (2001) Genetic Data Analysis: computer program for the analysis of allelic data. Version 1.0 (d

Introduction Spermatozoa are extremely sensitive cells and in recent years, male infertility deterioration has been recorded globally [ 1 ]. Examination of male infertility factor is represented mainly by a complete semen analysis. Nevertheless, examination of morphology, motility, concentration and total sperm count is not sufficient nowadays and other sperm parameters are observed more and more often. Despite being protected from adverse effects at the beginning of spermatogenesis, sperm DNA can be significantly fragmented in several ways. Sperm cells and their

. − SMAL-LA, K. 2001. Bacterial diversity of the rhizosphere of maize ( Zea mays ) grown in tropical soil stud-ied by temperature gradient gel electrophoresis. In Plant Soil , vol. 232 , 2001, pp. 167−180. DOI: 10.1023/A:1010350406708. LIU, W.T. − MARSH, T.L. − CHENG, H. − FORNEY, L.J. 1997. Characterization of microbial diversity by de-termining terminal restriction fragment length poly-morphisms of genes encoding 16S rRNA. In Applied and Environmental Microbiology , vol. 63 , 1997, pp. 4516−4522. MARSCHNER, P. − RUMBERGER, A. 2004. Rap-id changes in the rhizosphere

Abstract

The East Balkan Peninsula Area was a part from the Tethys Ocean until 72 000 000 years. The pre Maestrichtian geologic-tectonic pattern of cockle of the East Balkan Peninsula Area wasn’t built on the Europe Continental Massif. The modern East Balkan Peninsula Relief is forming during the Late Quaternary time. The East Balkan Peninsula Margin coincides with the border between the Bulgarian and Moesian Continental Microplates from the west and the Black Sea Oceanic Microplatte to the east. This border present the Neo Europe West Passive Continental Margin in the area of the last Tethys Oceanic Fragment – it Black Sea Oceanic Gulf.

continuous forest, forest fragments and in an agricultural mosaic habitat-island at Los Tuxtlas, Mexico. Biological Conservation 103: 237–245. DOI 10.1016/S0006-3207(01)00135-5. Ethier K., Fahrig L. 2011. Positive effects of forest fragmentation, independent of forest amount, on bat abundance in eastern Ontario, canada. Landscape Ecology 26: 865–876. DOI 10.1007/s10980-011-9614-2. Fuszara M., Cygan J. P. 1994. Nowe stanowisko nocka Bechsteina, Myotis bechsteini (Kuhl, 1818) w centralnej Polsce. Przegląd Zoologiczny 38: 335–337. Fuszara M., Kowalski M. 2009. Nocek

References BÄRWOLF M., 2006: Soil properties. In: Measurements of Max Planck Inst. fűr Biogeochemistry at the Tatra windthrow sites. Jena, CD-ROM. BRAKENSIEK D. L., RAWLS W. J. and STEPHENSON G. R., 1986: Determining the saturated hydraulic conductivity of soil containing rock fragments. Soil Sci. Soc. Am. J., 50 , 834-835. BROUWER J., ANDERSON H., 2000: Water holding capacity of ironstone gravel in a typic Phlintoxeralf in Souteast Australia. Soil Sci. Soc. Amer. J., 64 , 1603-1608. COILE T. S., 1953: Moisture content of small stone in soils. Soil Science, 75

-restriction fragment length polymorphism (PCR-RFLP) analyses. Routine Biochemical Tests Serum ALT and serum AST were determined based on the method of Henry et al . [ 17 ] and Amador et al . [ 18 ] respectively using kits supplied by Roche Diagnostics GmbH (Penzberg, Germany). Serum albumin was determined using kits also supplied by Roche Diagnostics GmbH. Serum alkaline phosphatase (ALP) was determined based on the method of Bretaudiere et al . [ 19 ] using kits supplied by Bio Diagnostic (Giza, Egypt). Serum bilirubin was detected based on the method of Landis et al . [ 20

References Anderson J., Rowcliffe J.M., Cowlishaw G. 2007. Does the matrix matter? A forest primate in a complex agricultural landscape. Biological Conservation 135: 212–222 DOI 10.1016/j.biocon.2006.10.022. Antongiovanni M., Metzger J.P. 2005. Influence of matrix habitats on the occurrence of insectivorous bird species in Amazonian forest fragments. Biological Conservation 122: 441–451. DOI 10.1016/j.biocon.2004.09.005. Arroyo-Rodríguez V., González-Perez I.M., Garmendia A., Solá M., Estrada A. 2013. The relative impact of forest patch and landscape