Krystyna Pohorecka, Andrzej Bober, Marta Skubida, Dagmara Zdańska and Kamila Torój
., Pettis J. S., Lipkin W. I. (2007) A metagenomic survey of microbes in honey bee colonycollapse disorder. Science 318: 283-287. DOI: 10.1126/science.1146498
Currie R. W., Gatien P. (2006) Timing acaricide treatments to prevent Varroa destructor (Acari: Varroidae) from causing economic damage to honey bee colonies. Canadian Entomologist 138: 238-252.
Dainat B., Evans J. D., Chen Y. P., Gauthier L., Neumann P. (2012a) Predictive markers of honey bee colonycollapse. PLoS ONE 7: e32151.
Dainat B., Evans J. D., Chen Y. P
Declines in honeybee (Apis mellifera ) colonies have elicited great concern worldwide. Recently, many Japanese beekeepers have implied that midsummer use of a new insecticide, neonicotinoid, in rice fields, is causing widespread mortality of neighboring honeybees and frequently resulting in colony collapse. Since few field experiments have directly tested the effects of neonicotinoids, I addressed four research questions in the field. The questions are: 1) Does clothianidin application in rice fields cause the collapse of neighboring honeybee colonies? 2) Is colony collapse related to hive distance from the rice field? 3) Is the number of dead honeybee workers after spraying, related to hive distance from the field? 4) What are the long-term effects of neonicotinoid use on honeybee colony growth, especially brood production? In the late summer of 2010, honeybee hives were placed adjacent to two separate rice fields for 1 week. The hives were placed at the distance of 0, 30, 60, and 90 m. After spraying clothianidin, a daily count of dead worker honeybees was done for a week. Hives were weighed, and capped-brood areas were estimated weekly, for 2 months following insecticide application. Although the average number of dead workers ranged from 40 to over 100 within 24 hours after spraying, only a few dead workers were observed in the subsequent days. Distance from the rice field had no significant effect on the number of dead workers. There were no collapsed colonies during the 2-month, post-spray observation period. Hive weight and capped-brood area did not significantly differ among those hives placed at varying distances from the rice field. These results indicated that clothianidin spraying of the rice field increased the mortality of the honeybees, but did not always cause colony collapse.
Zoran Stanimirović, Uroš Glavinić, Marko Ristanić, Nevenka Aleksić, Nemanja Jovanović, Branislav Vejnović and Jevrosima Stevanović
losses, managed colony population decline, and colonycollapse disorder in the United States. J Apicult Res 2010, 49:134-136.
12. Jacques A, Laurent M, EPILOBEE Consortium, Ribière-Chabert M, Saussac M, Bougeard S, Budge GE, Hendrikx P, Chauzat M: A pan-European epidemiological study reveals honey bee colony survival depends on beekeeper education and disease control. PLoS ONE 2017, 12:e0172591.
13. Brodschneider R, Gray A, Adjlane N, Ballis A, Brusbardis V, Charrière JD, Chlebo R, Coffey MF, Dahle B, de Graaf DC, Dražić MM, Evans G, Fedoriak M, Forsythe I
Marek Farjan, Krystyna Żółtowska, Zbigniew Lipiński, Elżbieta Łopieńska-Biernat and Małgorzata Dmitryjuk
The colony collapse disorder is a growing problem world-wide. For this reason, we were prompted to search for natural and harmless agents that could improve the living conditions of honey bees. This group of agents includes exogenous antioxidants, such as ascorbic acid, which boost natural immunity. We analysed the effect of vitamin C supplementation on carbohydrate metabolism in the developing honey bee worker brood. The total carbohydrate content and the concentrations of glycogen, trehalose, maltotriose, fructose, and glucose were estimated. The correlations between sugar content and the activity of the main hydrolases of carbohydrate metabolism - α-amylase, glucoamylase, trehalase, maltase, and sucrase - were determined. The addition of vitamin C to the diet of wintering bees did not impair their sugar metabolism. Vitamin C supplements exerted a positive effect by significantly increasing glycogen and trehalose concentrations in the initial phase of development and in newly emerged workers. Vitamin C did not induce significant changes in the developmental profile of carbohydrate degrading enzymes, except for the earliest stage of larval development when enzyme activity levels were below those noted in the control group.
SK, Simons JF, Egholm M, Pettis JS, Lipkin WI: A metagenomic survey of microbes in honey bee colonycollapse disorder. Science 2007, 318:283-287.
16. Van der Zee R: Colony losses in the Netherlands. J Apic Res 2010, 49:121-123.
17. Higes M, Martín-Hernández R, Garrido-Bailón E, Botias C, García-Palencia P, Meana A: Regurgitated pellets of Merops apiaster as fomites of infective Nosema ceranae (Microsporidia) spores. Environ Microbiol 2008, 10:1374-1379.
18. Valera F, Martín-Hernández R, Higes M: Evaluation of large
., Egholm M., Pettis J. S., Lipkin W. I. (2007) - A metagenomic survey of microbes in honey bee colonycollapse disorder. Science , 318: 283-287.
De Miranda J. R., Cordoni G., Budge G. (2010) - The Acute bee paralysis virus-Kashmir bee virus-Israeli acute paralysis virus complex. J. Invert. Pathol. , 103: 30-47.
Drummond A. J., Ashton B., Buxton S., Cheung M., Cooper A., Duran C., Field M., Heled J., Kearse M., Markowitz S., Moir R., Stones-Havas S., Sturrock S., Thierer T., Wilson A. (2011) - Geneious v5.4, [online
Şapcaliu Agripina, Vasilică Savu, Ion Radoi, Dana Tapaloagă, Petruţ Tanase and Victor Calin
) and contaminates honey in Australia, Apidologie 2009, 40: 117-123.
8. Higes M, Martin-Hernandez R, Botias C, Bailon E.G, Gonzalez-Porto A.V, Barrios L, Jesus del Nozal M, Bernal JL, Jimenez, JJ, Palencia P.G, Meana A. How natural infection by Nosema ceranae causes honeybee colonycollapse, Environ. Microbiol. 2008b, 10: 2659-2669.
9. Savu Vasilică, Şapcaliu Agripina, Patologia albinelor, Editura Fundaţiei România de Mâine, 2013, vol. 1: 134-153.
10. Chen Y, Evans JD, Smith IB, Pettis JS. Nosema ceranae is a long