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relationship between von Willebrand disease and haemophilia as a key to unlocking the secrets of factor VIII, a theme he pursued under Leon Hoyer in Connecticut (1976/7) then in London at The Royal Free Hospital where he was appointed successor to Katharine Dormandy. By 1982 total purification of factor VIII using monoclonal antibodies was achieved by his team in sufficient quantity for protein sequencing, leading to cloning and synthesis in collaboration with Genentech in 1984. From 1987 to 2005 he led an MRC haemostasis research group which achieved many advances in the

different cancers that contributed to tumourigenesis and cancer progression ( 21 ). Even though Cdc42 is closely associated with infectious diseases and cancers; there are no reports about the relationship between Cdc42 and brucellosis until now. In our previous study, a subtractive cDNA library of buffy coat from Brucella -infected and vaccinated sheep was constructed by suppression subtractive hybridisation (SSH), and a partial cDNA of Cdc42 gene was screened and sequenced. In this study, we cloned Cdc42 from sheep (OaCdc42) by rapid amplification of cDNA ends (RACE

References 1. Choumerianou DM, Dimitriou H, Kalmanti M. Stem cells: Promises versus limitations. Tissue engineering. Part B, Reviews. 2008; 14:53-60. 10.1089/teb.2007.0216 2. Sidhu KS, Ryan JP, Tuch BE. Derivation of a new human embryonic stem cell line, endeavour-1, and its clonal propagation. Stem Cells Dev. 2008: 17:41-51. 10.1089/scd.2007.0055 3. Munsie, MJ, Michalska AE, O’Brien CM, Trounson AO, Pera MF; Mountford PS. Isolation of pluripotent embryonic stem cells from reprogrammed adult mouse somatic cell nuclei. Curr Biol. 2000; 10:989-92. 10.1016/S0960

replaced, and the remaining cells were cultured for five days in the atmosphere with 5% CO 2 . Cloning of PK15 cells by limiting dilution . Well-grown PK15 cells were trypsinised and collected. Then, 100 μL of cells were transferred to 96-well plates precoated with PM feeder cells at a density of 5 cells/mL; seeding an average of 0.5 cells/well ensured that some wells received a single cell. Cells were incubated at 37°C for 7–14 days. When cell growth in the bottom of the wells was 50% confluent, the subclones from each plate were transferred into new 96-well plates and

antagonists in the treatment of HIV. Expert Opin. Investig. Drugs , 12 (2), 185-195. Gupta, S. K., Pillarisetti, K. (1999). Cutting edge: CXCR4-Lo: molecular cloning and functional expression of a novel human CXCR4 splice variant. J. Immunol. , 163 (5), 2368-2372. Heesen, M., Berman, M. A., Benson, J. D., Gerard, C., Dorf, M. E. (1996). Cloning of the mouse fusin gene, homologue to a human HIV-1 co-factor. J. Immunol. , 157 (12), 5455-5460. Holm, N. T., Byrnes, K., MacDonald, M., Abreo, F., Ampil, F., Sanders, M., Burton, G. V., Li, B., Chu, Q. (2007). Overexpression

References BURDON, R. D. and L. D. GEA (2006): Pursuit of genetic gain and biotic risk management for Pinus radiata in New Zealand. pp. 133-140. In: ‘Breeding for Success: Diversity in Action’, edited by C. F. MERCER. Proc. 13th Australasian Plant Breeding Conference, Christchurch, New Zealand 18-21 April 2006.. BURDON, R. D. (1986): Clonal forestry and breeding strategies - a perspective. pp. 645-659 in Proc. IUFRO Working Parties on Breeding Theory, Progeny Testing and Seed Orchards. Williamsburg, Va., USA. October 13 1986. BURDON, R. D. and C

is allosteric regulation is similar but not identical to that of fructose-1,6-bisphosphatase. Photosynth. Res. 58: 221-230. FUJIMORI, K., S. TADA, S. KANAI and D. OHTA (1998): Molecular cloning and characterization of the gene encoding N´-[(5´-phosphoribosyl)-formimino]-5-aminoimidazole-4- carboxamide ribonucleotide (BBM II) isomerase from Arabidopsis thaliana. Mol. Gen. Gent. 259: 216-223. GEIGER, D. R. and J. C. SERVAITES (1995): Diurnal regulation of photosynthetic carbon metabolism in C3 plants. Annu. Rev. Plant Physiol. Plant Mol. Biol. 45: 235-56. HANNAERT, V

fermentation progress of soy sauce through dynamic changes of volatile compounds. Foood Research International, 53(1): 189-194. DOI: 10.1016/j.foodres.2013.04.001 14. Wu GJ, Wu GB, Zhan T, Liu Z (2013) Characterization of a cold-adapted and salt-tolerant esterase from a psychrotrophic bacterium Psychrobacter pacificensis . Extremophiles, 17(5): 809-819. DOI: 10.1007/s00792-013-0562-4 15. Xu SQ, Hu YF, Yuan AH, Zhu BL (2010) Cloning, expression and characterization of a novel esterase from marine sediment microbial metagenomics library. Acta Microbiologica Sinica, 50(7): 891

. 40 (1), 6–75. Simberloff, D. 1996. Hybridization between native and introduced wildlife species: importance for conservation. Wildlife Biology , 2 (3), 143–150. Stöck, M., Ustinova, J., Betto-Colliard, C. et al. 2012. Simultaneous mendelian and clonal genome transmission in a sexually reproducing, all-triploid vertebrate. Proc. R. Soc. B ., 279, 1293–1299. Yatsyk, A. V. 2004. The ecology of water resources . Vol. 3 (5) . Geneza, Kyiv, 1–496 [In Ukrainian].

References Anger M., Kues W.A., Klima J., Mielenz M., Kubelka M., Motlik J., Esner M., Dvorak P., Carnwarth J.W., Niemann H. (2003). Cell cycle dependent expression of Plk1 in synchronized porcine fetal fibroblasts. Mol. Reprod. Dev., 65: 245-253. Boquest A.C., Grupen C.G., Harrison S.J., Mc Ilfatric k S.M., A s h m a n R.J., d’Apice A.J.F., Nottle M.B. (2002). Production of cloned pigs from cultured fetal fibroblast cells. Biol. Reprod., 66: 1283-1287. Brunetti D., Perota A., Lagutina I., Colleoni S., Duchi R., Calabrese F., Seveso M., Cozzi E., Lazzari G