Tomáš Perečko, Katarína Drábiková, Radomír Nosáľ, Juraj Harmatha and Viera Jančinová
Perecko T, Jancinova V, Drabikova K, Nosal R and Harmatha J. (2008). Antioxidative effect of derivatives of stilbene. Comparison of trans-resveratrol, pinosylvin and pterostilbene. Interdiscip Toxicol 1 : 106.
Pongracz J, Webb P, Wang K, Deacon E, Lunn OJ and Lord JM. (1999). Spontaneous neutrophil apoptosis involves caspase3-mediated activation of protein kinase C-delta. J Biol Chem 274 : 37329-37334.
Radhakrishnan S, Reddivari L, Sclafani R, Das UN and Vanamala J. (2011). Resveratrol potentiates grape seed extract induced human
Ludwik Jasinski, Patrycja Chylinska-Wrzos, Marta Lis-Sochocka, Ewelina Wawryk-Gawda and Barbara Jodlowska-Jedrych
1. Abu-Qare A.W., Abou-Donia M.B.: Biomarkers of apoptosis: release of cytochrome c, activation of caspase-3, induction of 8-hydroxy-2’- deoxyguanosine, increased 3-nitrotyrosine, and alteration of p53 gene. Journal of Toxicology and Environmental Health, 4, 313, 2001.
2. Adachi J. et al.: The Development of Fulminant Type 1 Diabetes During Chemotherapy for Rectal Cancer. Intern Med., 54, 819, 2015.
3. Anuradha R. et al.: Apoptosis of beta cells in diabetes mellitus. DNA Cell Biol., 33, 743, 2014
Eduardo Berenguer, María-Teresa Solís, Yolanda Pérez-Pérez and Pilar S. Testillano
, Gallois P, Zavialov A, Lam E, Bozhkov PV. Metacaspases. Cell Death and Differentiation. 2011; 18(8): 1279.
34. Danon A, Rotari VI, Gordon A, Mailhac N, Gallois P. Ultraviolet-C overexposure induces programmed cell death in Arabidopsis, which is mediated by caspase-like activities and which can be suppressed by caspase inhibitors, p35 and Defender against Apoptotic Death. Journal of Biological Chemistry. 2004; 279(1): 779-87.
35. Ge Y, Cai YM, Bonneau L, Rotari V, Danon A, McKenzie EA, McLellan H, Mach L, Gallois P. Inhibition of cathepsin B by caspase-3
Dorota Bądziul, Barbara Januszanis, Joanna Jakubowicz-Gil, Ewa Langner, Tomasz Piersiak, Wojciech Rzeski and Antoni Gawron
Localization of caspase-3, caspase-8, cytochrome c, Hsp27, Hsp72 and LC3 in glioma cells upon quercetin and Temozolomide treatment
It is well known that the basis of antitumor activity of compounds is the induction of programmed cell death. At the base of this process is the activation and proper localization of marker proteins. Therefore, the aim of our studies was to determine the effect of quercetin and Temozolomide, used separately or in combinations on the localization of proapoptotic proteins such as cytochrome c, caspase-3, caspase-8, antiapoptotic Hsp27, Hsp72 and proautophagal LC3 in MOG-GCCM, human Anaplastic astrocytoma cell line.
Incubation with quercetin stimulated the translocation of caspase-3 and Hsp72 from the cytoplasm to the nucleus. Temozolomide changed the localization of caspase-3 and Hsp72, causing migration of this protein from the cytoplasm to the nuclei. In the Temozolomide-treated cells caspase-8 was detected mainly in the cytoplasm. Quercetin and Temozolomide administered in combination caused changes in the distribution of tested proteins. Cells treatment with both drugs showed cytoplasmic localization of caspase-8 and nuclear localization of cytochrome c. In cells incubated with quercetin and Temozolomide Hsp27 was detected in the whole cell. In the case of cytoplasmic LC3 we did not notice any significant changes in distribution.
Piotr A. Antos, Anna Hrabia, Anna Gdula and Andrzej Sechman
development is preceded byadecrease in myocyte proliferation and an increase in cardiac apoptosis. Teratology, 64: 201-212.
Johnson A.L. (1996). The avian ovarian hierarchy:abalance between follicle differentiation and atresia. Poultry Avian Biol. Rev., 7: 99-110.
Johnson A.L., Bridgham J.T. (2000). Caspase-3 and -6 expression and enzyme activity in hen granulosa cells. Biol. Reprod., 62: 589-598.
Johnson A.L., Bridgham J.T. (2002). Caspase-mediated apoptosis in the vertebrate ovary. Reprod., 124: 19
Viera Jančinová, Tomáš Perečko, Radomir Nosáľ, Danica Mihalová, Katarína Bauerová and Katarína Drabiková
rat kidney. Basic Clin Pharmacol Toxicol 99 : 329-334.
Perečko T, Drábiková K, Račková L, Čáž M, Podborská M, Lojek A, Harmatha J, Šmidrkal J, Nosáľ R, Jančinová V. (2010). Molecular targets of the natural antioxidant pterostilbene: effect on protein kinase C, caspase-3 and apoptosis in human neutrophils in vitro. Neuroendocrinol Lett 31 : 84-90.
Rossi AG, Hallett JM, Sawatzki DA, Teixeira MM, Haslett C. (2007). Modulation of granulocyte apoptosis can influence the resolution of inflammation. Biochem Soc
Mustafa Ozkaraca, Songul Ceribasi, Ali Osman Ceribasi, Ayse Kilic and Hasan Ongor
This study is aimed to evaluate the relationship between the severity of apoptotic and autophagic cell death based on the distribution of Brucella spp. antigens in the lung, liver, kidney, spleen, brain, heart, skeletal muscle, mesenteric lymph node, and thymus tissue from bovine fetuses aborted due to natural infection with Brucella spp. The distribution of Brucella spp. antigens was immunohistochemically examined in the tissues of 16 aborted fetuses from cattle diagnosed with Brucella spp. infection by a polymerase chain reaction (PCR). In addition, immunostaining of primary antibodies for cleaved caspase 3 was performed to detect apoptosis, and immunostaining of Microtubule Associated Protein 1 Light Chain 3 Beta (LC3B) was used to detect autophagy in the Brucella spp.- related abortions. Analysis of cellular death revealed strong immunopositivity in the lung, spleen, kidney, and thymus, moderate immunopositivity in the liver, mesenterial lymph nodes, and heart muscle and slight immunopositivity in the brain and skeletal muscle by staining of Brucellaspp. antigens. According to the immunohistochemical results, the immunopositivity of cleaved caspase 3 and LC3B was extremely high in the lung, thymus, spleen, kidney, and liver tissues. The immunostaining of cleaved caspase 3 in the lung, thymus, and kidney tissues was severe compared to that of LC3B. In the liver, spleen, and mesenterial lymph nodes, the immunopositivity of LC3B was higher than that of cleaved caspase 3. Bacterial antigens were highly evident in the lung, spleen, kidney, and thymus tissues of Brucella spp.-related bovine abortions, and both apoptosis and autophagy played a role in cellular death.
Jelena Danilović Luković, Aleksandra Korać, Ivan Milošević, Tijana Lužajić, Zorana Milanović, Milica Kovačević Filipović and Anita Radovanović
Induced prenatal hypothyroidism in rat pups leads to accelerated primordial follicle assembly and premature follicular atresia with ovary failure. This work investigates the influence of maternal hypothyroidism induced with 6-n-propyl-2-thyouracil (PTU) on the number and morphology of oogonia/oocytes in newborn rat pups with light and transmission electron microscopy. Expression of apoptosis and autophagy markers in oogonia/oocytes were examined using immunohistochemistry. Hypothyroid newborn pups had a decreased number of mitotic and resting oogonia, while the number of altered oogonia/oocytes was increased. Ultrastructural observations revealed the increased presence of degenerated pachytene oocytes (Z-cells) and oogonia/oocytes undergoing autophagy, apoptosis and combined apoptosis and autophagy, in this group. The most abundant altered oogonia/oocytes in the hypothyroid group were those with morphological features of advanced autophagy and Z-cells. The percentage of TUNEL (terminal deoxynucleotidyl transferase dUTP nick end labeling) positive oogonia/oocytes was significantly lower in the hypothyroid group. No significant difference was recorded in the expression of caspase-3, ATG7 and LC3 possibly reflecting that these proteins were not involved in the oogonia/oocyte alteration process during prenatal rat hypothyroidism. The obtained results indicate that developmental hypothyroidism in the offspring enhances the number of Z-cells and oogonia/oocytes altered with the advanced process of autophagy.