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Microbiological Risk Assessment and Bioprocess Engineering

process safety in bioprocess manufacturing facilities. EU- OSHA (European Agency for Safety and Health at Work) (2010). ESENER - managing safety and health at work. Available at: http://osha.europa.eu/en/publications/reports/esener1_osh_management EU-OSH Priorities for occupational safety and health research in Europe: 2013-2020 (2013). Publications Office of the European Union. ISBN: 978-92-9240-068-2 doi: 10.2802/25457. EU-OSHA (European Agency for Safety and Health at Work) (2011). Foresight of new and emerging risks

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Nonlinear Stabilizing Control of an Uncertain Bioprocess Model

). Mathematical modelling and parameter estimation of anaerobic fermentation processes, Bioprocess Engineering   21 (4): 377-381. Simeonov, I., Noykova, N. and Stoyanov, S. (2004). Modelling and extremum seeking control of the anaerobic digestion, Proceedings of the International IFAC Workshop DECOMTT, Bansko, Bulgaria , pp. 289-294. Simeonov, I., Noykova, N. and Gyllenberg, M. (2007). Identification and extremum seeking control of the anaerobic digestion of organic wastes, Cybernetics and Information Technologies   7 (2

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Influence of biological removal of lactose on the physical and chemical parameters of crude sweet whey

Abstract

The crude sweet whey is an effluent and a co-product of cooked and pressed cheeses and casein, released into the environment without prior treatment (case of Cheese Dairy Sidi Saada, Yellel, Relizane, Algeria) affect the quality of freshwater ecosystems (Oued Mina, Relizane, Algeria).Our study focused on the control of the physical and chemical parameters of crude sweet whey and delactosed whey.The results showed that the applied bioprocess modified the physical and chemical parameters of crude sweet whey such as :density,dry matter,refractive index,viscosity,ash,pH and electrical conductivity, acidity,proteins and lactose; for this purpose these findings depended on the operating conditions, and the composition of the whey put in treatment.

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Biochemical and cellular properties of Gluconacetobacter xylinus cultures exposed to different modes of rotating magnetic field

and biofilm formation by S. aureus and E. coli . J. Magn. 18(3), 289–296. DOI: 10.4283/JMAG.2013.18.3.289. 22. Lee, K.Y., Buldum, G., Mantalaris, A. & Bismarck, A. (2014). More than meets the eye in bacterial cellulose: biosynthesis, bioprocessing, and applications in advanced fiber composites. Macromol. Biosci. 14(1), 10–32. DOI: 10.1002/mabi.201300298. 23. Toyosaki, H., Naritomi, T., Seto, A. & Yoshinaga, F. (1995). Screening of bacterial cellulose-producing Acetobacter strains suitable for agitated culture. Biosci. Biotech. Biochem. 59

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Biorefineries – New Green Strategy For Development Of Smart And Innovative Industry

Abstract

Ecological engineering or ecotechnology is defined as the design of sustainable production that integrate human society with the natural environment for the benefit of both. In order to reach the goal of sustainability therefore important that bioproduct production systems are converted from to natural cycle oriented. In natural cycles there are not waste, but products are generated at different stages of the cycle. The ecotechnology creates a sustainable bioeconomy using biomass in a smart and efficient way. The biorefining sector, which uses smart, innovative and efficient technologies to convert biomass feedstocks into a range of bio-based products including fuels, chemicals, power, food, and renewable oils, currently presents the innovative and efficient bio-based production can revitalize existing industries. The paper presents the concept of biorefinery as the ecotechnological approach for creating a sustainable bioeconomy using biomass in a smart and efficient way.

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Effect of Fermentation on Structural Characteristics and Bioactive Compounds of Bee-Pollen Based Food

Abstract

Bee-pollen is a product of the hive which has had a growth in consumption in recent years due to the recognition of its nutritional and bioactive potential. However, several reports have shown that the external structure of the grain limits the absorption of nutrients in the human gastrointestinal tract. A structural modification could be achieved through fermentative processes, favoring the release of compounds found inside this food, in addition to obtaining a product with potential probiotic characteristics. The objective of this work was to evaluate how fermentation through the inclusion of yeasts of the species Saccharomyces cerevisiae, bacteria of species Lactobacillus plantarum or a commercial culture Choozit® affeccted such parameters as Scanning Electron Microscopy (SEM), Differential Scanning Calorimetry (DSC), phenolic compounds, flavonoids and antioxidant activity. The results found that the use of consortia between yeast and lactic acid bacteria significantly increased in such characteristics as total phenolics and antioxidant activity by 31% and 39% respectively. The analysis by DSC showed an increase in the heat flow of the fermented products compared to fresh bee-pollen, which could indicate structural modification caused by the activity of microorganisms, a fact made visible through micrographs obtained by Scanning Electron Microscopy.

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Studies on poly-3-hydroxyoctanoate biosynthesis by a consortium of microorganisms

Abstract

Polyhydroxyalcanoates (PHAs) are specifically produced by a wide variety of bacteria, as an intracellular energy reserve in the form of homo- and copolymers of [R]-β-hydroxyalkanoic acids, depending on the C source used for microorganism growth, when the cells are grown under stressing conditions. In this paper we present microbiological accumulation of poly-3-hydroxyoctanoate (PHO) by using a consortium of bacterial strains, Pseudomonas putida and Bacillus subtilis, in a rate of 3:1, grown on a fermentation medium based on sodium octanoate as the sole carbon source. The experiments performed in the above mentioned conditions led to the following results: from 18.70 g sodium octanoate (7.72 g/L in the fermentation medium) used up during the bioprocess, 3.93-3.96 g/L dry bacterial biomass and 1.834 - 1.884 g/L PHA, containing 85.83 - 86.8% PHO, were obtained.

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Influence of Light Intensity and Temperature on Cultivation of Microalgae Desmodesmus Communis in Flasks and Laboratory-Scale Stirred Tank Photobioreactor

Abstract

Optimization of the microalgae cultivation process and of the bioprocess in general traditionally starts with cultivation experiments in flasks. Then the scale-up follows, when the process from flasks is transferred into a laboratory-scale bioreactor, in which further experiments are performed before developing the process in a pilot-scale reactor. This research was done in order to scale-up the process from a 0.4 1 shake flask to a 4.0 1 laboratory-scale stirred-tank photobioreactor for the cultivation of Desmodesmus (D.) communis microalgae. First, the effect of variation in temperature (21-29 ºC) and in light intensity (200-600 μmol m-2s-1) was studied in the shake-flask experiments. It was shown that the best results (the maximum biomass concentration of 2.72 g 1-1 with a specific growth rate of 0.65 g g-1d-1) can be achieved at the cultivation temperature and light intensity being 25 °C and 300 μmol m2s-1, respectively. At the same time, D. communis cultivation under the same conditions in stirred-tank photobioreactor resulted in average volumetric productivities of biomass due to the light limitation even when the light intensity was increased during the experiment (the maximum biomass productivity 0.25 g 1-1d-1; the maximum biomass concentration 1.78 g 1-1).

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Design of operational temperature for immobilized glucose isomerise using an accelerated inactivation method

(2004) Biochimie 86: 561–568. Converti A, Del Borghi M (1997) Enzyme Microb. Technol. 21: 511–517. Converti A, Del Borghi M (1998) Bioprocess Eng. 18: 27–33. Daniel RM, Danson MJ, Eisenthal R (2001) Trends Biochem. Sci. 26: 223–225. DiCosimo R, McAuliffe J, Poulose AJ, Bohlmann G (2013) Chem. Soc. Rev. 42: 6437–6474. Gibbs PR, Uehara CS, Neunert U, Bommarius AS (2005) Biotechnol. Prog. 21: 762–774. Illeová V, Polakovič M, Štefuca V, Ačai P, Juma M (2003) J. Biotechnol. 105: 235–243. Lim LH, Saville BA (2007) Appl. Biochem

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Polyurethanes used in the endoprosthesis of joints

). Biodegradable Molecularly Imprinted Polymers Based on Poly(ε-caprolactone). Biotechnology and Bioprocess Engineering 12, 152-156. PL Patent Application No. P-384883.

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