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Abstract

The paper presents results of microbiological research of plastic elements. Plastic elements were made of the polystyrene composition with addition of nanosilver and nanocopper with a laboratory extruder EHP 25ELine. Microbiological research was carried with regard to evaluation of antifungal impact of nanoadditives according to international standards with Cryobank (Mast Diagnostica). A positive impact of nanoadditives on antifungal properties of polystyrene elements produced by means of extrusion which is very favourable in the aspect of their use in agri-food processing.

locals in Kishtwar, Jammu and Kashmir, India. -Ethnobotanical Leaflets, 13: 1240-1256. Knobloch K., Pauli A., Iberl B., Weigand H., Weis N., 1989: Antibacterial and antifungal properties of essential oil components. - Journal of Essential Oil Research, 1(3): 119-128. Maharjan B.L., Mainali S., Baral B., 2011: Phy-tochemical screening and antimicrobial assay of some Nepalese medicinal plants. - Scientific World, 9(9): 90-92. Moshi M.J., 2005: Current and future prospects of integrating traditional and alternative medicine in the management of diseases in Tanzania

Abstract

Lactic acid bacteria are frequently utilized in food industry and they are also recognized as antimicrobial agents due to their capability to produce metabolites such as: organic acids, biosurfactants, bacteriocins, hydrogen peroxide, cyclic dipeptides, exopolysaccharides. The main goal of this paper was to present the results of the research carried out on the strain LCM2 of lactic acid bacteria isolated from brined cucumbers, for production of biosurfactants and to assess its antifungal properties. The emulsification capacity of biosurfactant was measured using kerosene as the hydrophobic substrate. The value of emulsification index E24 was 89.04% showing a high emulsification activity of the biosurfactant. The structural characterization of biosurfactant by TLC revealed its glycolipidic nature. Assay of the ionic charge established the anionic charge of the biosurfactant revealed by the presence of precipitation lines towards the cationic surfactant dodecyl-dimethyl-ammonium chloride. The biosurfactant presented antibiofilm activity with low adherence capacity, structural damages of the hyphal net, conidiophores and delays or lack of sporulation and decreased biomass accumulation in four mycotoxigenic Penicillium and Aspergillus isolates. Results of in vitro assays recommend the biosurfactant produced by the new lactic acid bacteria strain LCM2 for biotechnological purposes, as alternative antifungal agent in food industry.

Abstract

The biological and photocatalytic activity of TiO2 and TiO2:Cu in relation to their structure, surface topography, wettability and optical properties of the thin films was investigated. Thin-film coatings were prepared by magnetron sputtering method in oxygen plasma with use of metallic targets (Ti and Ti-Cu). The results of structural studies revealed that addition of Cu into titania matrix (during the deposition process) resulted in obtaining of an amorphous film, while in case of undoped TiO2, presence of nanocrystalline anatase (with crystallites size of 20 nm) was found. Moreover, an addition of cooper had also an effect on surface diversification and decrease of its hydrophilicity. The roughness of TiO2:Cu film was 25 % lower (0.6 nm) as-compared to titania (0.8 nm). These modifications of TiO2:Cu had an impact on the decrease of its photocatalytic activity, probably as a result of the active surface area decrease. Antibacterial and antifungal properties of the thin films against bacteria (Enterococcus hirae, Staphylococcus aureus, Bacillus subtilis, Escherichia coli) and yeast (Candida albicans) were also examined. For the purpose of this work the method dedicated for the evaluation of antimicrobial properties of thin films was developed. It was revealed that Cu-additive has a positive impact on neutralization of microorganisms.

Abstract

Application of different kinds of mineral waters and peloids on the skin exerts mechanical, thermal and chemical effects. Significant reduction of inflammation and increased differentiation of keratinocytes may explain why balneotherapy has positive clinical effects in psoriatic patients. In vitro models have shown that thermal water stimulates interleukin-2 production after cell stimulation by staphylococcal enterotoxin B, and reduces interleukin-4 secretion. After balneotherapy, a significant decrease in Psoriasis Area Severity Index (PASI), associated with a significant reduction of interleukin-8, Staphylococcus aureus colonization and enterotoxin N, have been reported in patients with psoriasis. Mineral water was found to have inhibitory in vitro effects on substance P, TNF-α release and antigen-induced cell degranulation. Immunomodulatory effects of water depend on its content. Sulfur waters have beneficial anti-inflammatory, keratolytic, and antipruriginous effects and also possess antibacterial and antifungal properties. The effectiveness of balneotherapy in the treatment of psoriasis has been reported in many studies conducted all over the world. The majority of studies were conducted at the Dead Sea coast. Investigations showed that balneotherapy factors are important therapeutic factors in the treatment of psoriatic patients. The first and only comparable study of this kind in Serbia, was conducted in Prolom Spa with satisfactory therapeutic results.

SUMMARY

Black shank is a major annual disease threat to all types of tobacco worldwide. It is caused by the fungus Phytophthora parasitica var. nicotianae (PPN). The major tobacco growing areas in US - Kentucky, Tennessee and North Carolina can experience devastating losses, reaching in some fields up to 100%. Thus far, the main approaches to control this disease have been creation of resistant varieties, fungicide treatments, and crop rotation. Some fungicides are reported to have negative effects on the environment. The goal of this work was to test the antifungal activity of several natural products that are synthesized by certain Nicotiana species, and secreted to the leaf surface. We hypothesized that phylloplanin, cis-abienol, labdenediol and sclareol can suppress PPN-race 0- and PPN-race 1-caused disease in Burley tobaccos KY 14 and MS KY 14 × L8LC in the greenhouse. We developed methods for leaf surface extraction, spore preparation and soil drench application of the natural compounds tested. Experiments were performed on 5–8 week-old greenhouse grown seedlings. cis-Abienol showed high inhibitory properties toward the disease. Race 0 infection was completely subdued in KY 14 while race 1 infection was reduced by 70–80%, and delayed by 6–10 days in KY 14 and MS KY14 × L8LC. Sclareol was very effective in inhibiting race 0-caused disease in both tobacco cultivars. In MS KY 14 × L8LC race 1 infection was inhibited while in KY 14 it was reduced by 85% and delayed by 6 days. Labdenediol reduced the disease by half in eight week-old KY 14 plants. Tobacco phylloplanin reduced plant infection by both races by 50–60% and delayed the disease by 6–10 days. Phylloplanin was least suppressive in both tobacco cultivars. We consider sclareol to be the best candidate for future studies due to its antifungal properties and availability. cis-Abienol, despite its good antifungal activity, is not feasible for large-scale use due to the production and stability limitations.

Abstract

The decrease of seed quality during storage is strongly associated with damage caused by free radicals. Some compounds of natural origin, known due to their antioxidative and antifungal properties, may be potentially used in organic production for seed treatment as an alternative to pesticides. The aim of the experiment was to study an ability of hydrogen peroxide and organic acid to improve germination, vigour and health of non-deteriorated and deteriorated zinnia seeds. Two seed samples, cv. Illumination and Orys, varying in initial infestation with fungi were tested. For deterioration seeds were kept at 30°C and 80% RH for 30 days. Seed quality tests were performed before and after deterioration for controls and seeds soaked in 3.0% hydrogen peroxide solution and in 1.0 and 5.0% solutions of ascorbic and lactic acids for 10, 30 and 60 min. The controls were untreated seeds, seeds soaked in 0.2% solution of Kaptan zawiesinowy 50 WP for 30 min and seeds soaked in distilled water for 10, 30 and 60 min. Treating zinnia seeds with organic acids more significantly affected seed germination and health after deterioration than before, and improvement of germination capacity was usually correlated with decrease of the percentage of abnormal diseased seedlings. Deterioration had no influence on mean germination time, whereas in particular cases treating seeds with hydrogen peroxide and organic acids negatively affected this parameter. After deterioration regardless of treatment increased number of seeds free from fungi. Lactic acid followed by hydrogen peroxide and ascorbic acid effectively limited growth of A. alternata, A. zinnia and Fusarium spp. on zinnia seeds, however at higher concentration negatively affected seed germination and vigour. Moreover, treating seeds with hydrogen peroxide and organic acids many a time increased seeds infestation with B. cinerea.

Abstract

The main purpose of this study was to evaluate impact of plant growth promoting bacteria (PGPB) on the development of phytopathogenic fungi and correlate it with a potential effects on the growth of plants under unfavorable conditions, in order to improve the efficiency of a phytoremediation process. The conducted research focused on the antifungal properties of PGPB. In this study, 51 isolates of bacteria were obtained after diversified disinfection time from plants growing on soil after sewage sludge amendment. The results revealed that some isolated bacteria, mainly endophytic ones, inhibited the development of Fusarium oxysporum, F. culmorum and Alternaria alternata.

://dx.doi.org/10.1016/j.jep.2006.04.003 16. Duffy CF, Power RF. Antioxidant and antimicrobial properties of some Chinese plant extracts. Inter J Antimicrob Agent 2001; 17:527-529. 17. Rasooli I, Rezaei MB, Allameh A. Ultrastructural studies on antimicrobial efficacy of thyme essential oils on Listeria monocytogenes . Inter J Infect Diseases 2006; 10:236-241. doi: http://dx.doi.org/10.1016/j.ijid.2005.05.006 18. Izcan M, Boyraz N. Antifungal properties of some herb decoctions. Eur Food Res Technol 2000; 212:86-88. doi: http://dx.doi.org/10.1007/s002170000249 19. Ozcan M

German guidelines. J Saudi Soc Derm Derma Surg 2011;14:5-13. 9. Ibezimako Esimone CO, Ofokansi KC, et al. Evaluation of antifungal properties of nystatin-salicylic acid combinations against clinical isolates of candida albicans. J Biomed Invest 2003;1(1):10-7. 10. Teku RL, Mylangam CK, Kolapalli VR. Formulation of capsaicin-loaded emulgels using natural gums and oils for topical delivery. Wor J Pharm Pharma Sci 2015;5(1):1017-34. 11. Mori NM, Patel PV, Seth NR. Fabrication and characterization of voriconazole transdermal spray for the treatment of fungal infection. [M