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Detection of Narcissus Latent Virus Isolates Using One-Step Rt-Pcr Assay

. 1996. Silica capture-reverse transcription- polymerase chain reaction (SC-RT-PCR): application for the detection of several plant viruses. Proc. 4th Intern. EFPP Symp. Bonn, Germany, 9-12 September 1996, pp. 445-448. Mowat W.P., Dawson S., Duncan G.H., Robinson D.J. 1991. Narcissus latent, a virus with filamentous particles and novel combination of properties. Ann. Appl. Biol. 119: 31-46. Sochacki D. 2011a. The use of ELISA in the micropropagation of virus-free Narcissus. Acta Hort.. 886: 253-258. Sochacki D. 2011b

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Development and application of a TaqMan-MGB real-time RT-PCR assay for the detection of porcine epidemic diarrhoea virus strains in China

virus (TGEV) S gene. 4 th International Conference on Biomedical Engineering and Informatics (BMEI'11), 2011, Shanghai, pp. 1412–1415. 9. Huang Y.W., Dickerman A.W., Pineyro P., Li L., Fang L., Kiehne R., Opriessnig T., Meng X.J.: Origin, evolution, and genotyping of emergent porcine epidemic diarrhea virus strains in the United States. mBio 2013, 4, e00737–13. 10. Kim S.H., Kim I.J., Pyo H.M., Tark D.S., Song J.Y., Hyn B.H.: Multiplex real-time RT-PCR for the simultaneous detection and quantification of transmissible gastroenteritis virus and porcine

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RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer

RT-PCR Analysis of TOPBP1 Gene Expression in Hereditary Breast Cancer

Hereditary predisposition to breast cancer determined in large part by loss of function mutations in one of two genes BRCA1 and BRCA2. Besides BRCA1 and BRCA2 other genes are also likely to be involved in hereditary predisposition to breast cancer. TopBP1 protein is involved in DNA replication, DNA damage checkpoint response and transcriptional regulation. Expression of TopBP1 gene at the mRNA level was analyzed by semiquantitative reverse transcription-polymerase chain reaction (RT-PCR) in 94 samples of hereditary breast cancer. Analysis of TopBP1 mRNA level showed that expression of TopBP1 is significantly downregulated in poorly differentiated breast cancer (grade III according Bloom-Richardson system (P<0.05).

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Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses

Abstract

Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.

Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay.

Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results.

Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.

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Clinical Investigations. Comparison of Directigen Flu A+B with Real Time PCR in the Diagnosis of Influenza / Сравнение Иммунохроматографического Метода (Directigen Flu A+B) И Теста RT-PCR При Инфекциях Гриппа

multiplex one-step real time RT-PCR assay for influenza surveillance. Euro Surveill 2011;16(7):pii = 19798 13. Kaiser L, Briones MS and Hayden FG. Performance of virus isolation and Directigen Flu A to detect influenza A virus in experimental human infection. J Clin Virol 1999;14:191-7. 14. Reina J, Padilla E, Alonso F, de Gopegui ER, Munar M, Mari M. Evaluation of a new dot blot enzyme immunoassay (Directigen flu A+B) for simultaneous and differential detection of influenza A and B virus antigens from respiratory samples. J Clin Micro 2002

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Occurrence of reovirus (ARV) infections in poultry flocks in Poland in 2010–2017

south western Poland. Pol J Vet Sci 2014, 17, 299–305. 16. Yates V.J., Rhee Y.O., Fry D.E., El Mishad A.M., McCornick K.J.: The presence of avian adenoviruses and adeno-associated viruses in healthy chickens. Avian Dis 1976, 20, 146–152. 17. Zhang Y., Liu M., Shuidong O., Hu Q.L., Guo D.C., Chen H.Y., Han Z.: Detection and identification of avian, duck, and goose reoviruses by RT-PCR: goose and duck reoviruses are part of the same genogroup in the genus Orthoreovirus . Arch Virol 2006, 151, 1525–1538. 18. Zhong L., Gao L., Liu Y., Li K., Wang M., Qi

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Occurrence of Several Viruses Infecting Wild Growing Stone Fruit Trees in Central Bohemia

. Kumari S (2009): Detection of Cherry leaf roll virus and Strawberry latent ring spot virus by one-step RT-PCR. Department of Virology, 45, 140–143. Kunze L, Krczal H (1971): Transmission of sharka virus by aphids. In: Proceedings of the 8th European Symposium on Fruit Tree Virus Diseases. Paris, France: INRA, 255–260. Labonne G, Yvon M, Quiot JB, Avinent L, Llácer G (1995): Aphids as potential vectors of Plum pox virus : Comparison of methods of testing and epidemiological consequences. Acta Horticulturae, 386, 207–218. Marais A, Faure C

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Occurrence of Stone Fruit Viruses in Plum Orchards in Latvia

., Myrta, A., Llácer, G. (2006). Plum pox virus and estimated costs associated with sharka disease. Bulletin OEPP/EPPO Bulletin, 36 , 202-204. Capote, N., Bertolini, E., Olmos, A., Vidal, E., Martínez, M. C., Cambra, M. (2009). Direct sample preparation methods for detection of Plum pox virus by real-time RT-PCR. Int. Microbiol., 12 , 1-6. Çevik, B., Yardimci, N., Çulal-Klllç, H. (2011). Detection of viruses infecting stone fruits in Western Mediterranean region of Turkey. Plant Pathol. J., 27 (1), 44-52. Desvignes

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Diagnostic reliability of different RT-PCR protocols for the detection of bluetongue virus serotype 14 (BTV-14)

References 1. Anthony S., Jones H., Darpel K.E., Elliot H., Maan S., Samuel A., Mellor P.S, Mertens, P.P.C.: A duplex RT-PCR assay for detection of genome segment 7 (VP7 gene) from 24 BTV serotypes. J Virol Methods 2007, 141, 188–197. 2. DeMaula C.D., Bonneau K.R., MacLachlan N.J.: Changes in the outer capsid protein of bluetongue virus serotype 10 that abrogate neutralization by monoclonal antibodies. Virus Res 2000, 67, 59–66. 3. Elbers A.R., Backx A., Ekker H.M., van der Spek A.N., van Rijn, P.A.: Performance of clinical signs to detect

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Isolation and Molecular Detection of Bovine Parainfluenza Virus Type 3 in Cattle in Serbia

cattle. Journal of virological methods 2015, 222:47-54. 8. Lyon M, Leroux C, Chaestag T, Patet J, Mornex JF: Presence of a unique parainfluenza virus 3 strain identified by RT-PCR in visna-maedi virus infected sheep. Veterinary Microbiology 1997, 51:95-104. 9. Nišavić J, Milić N, Knežević A: Laboratorijska dijagnostika virusnih infekcija. In: Izolacija virusa u kulturi ćelija. Beograd, Srbija: Naučna KMD; 2013, 34-36. 10. Jian - Le Ren, Yuan - Mao Zhu, Yue - Hui Zhou, Chuang Lv, Hao Yan, Lei Ma, Hong - Fei Shi, Fei Xue

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