Viktor Bauer, Ružena Sotníková and Katarína Drábiková
activated neutrophils with the action of reactive oxygen species (ROS) on rat aortic smooth muscle (RASM). Prague Med Rep 109 (Suppl.): S13-14.
Bauer V, Sotníková R, Drábiková K. (2011). Effects of activated neutrophils on isolated rings of rat thoracic aorta. J Physiol Pharmacol 62 (5): 513-20.
Chatterjee M, Saluja R, Kanneganti S, Chinta S, Dikshit M. (2007). Biochemical and molecular evaluation of neutrophil NOS in spontaneously hypertensive rats. Cell Mol Biol 15 : 84-93.
Epy Muhammad Luqman, I Ketut Sudiana, Win Darmanto, Agung Budianto Achmad and Widjiati
maternal-placental-foetal unit. The effect of CF on the brain is more severe than the effect on other organs, particularly the liver ( 14 ).
Oral and intraperitoneal CF intake have proven to be able to increase reactive oxygen species (ROS) in adult mouse brains and increase the levels of malondialdehyde (MDA) ( 6 ). This was paralleled by an increase in MDA level in rat brains (59.38%) and in the mitochondria (43.7%) isolated from CF-exposed rat brains ( 12 , 25 ). The increasing ROS may reduce the activity of brain antioxidant enzymes such as superoxide dismutase
reduction in intracellular reactive oxygen species (ROS). For this reason, the change in PPP is directly related to the growth of cells. As solid tumors have unique low oxygen conditions that make cells that only adapt to the environment to survive, this forces tumor cells to metabolic reprogram for growth advantage. Warburg [ 2 ] discovered that differentiation of the normal cells takes place mainly using mitochondrial oxidative phosphorylation capacity. Hence, a vast majority of cancer cells rely on aerobic glycolysis and generate a lot of lactic acid, a phenomenon
Ivana Steiner, Nikolina Stojanovic, Aljosa Bolje, Anamaria Brozovic, Denis Polancec, Andreja Ambriovic-Ristov, Marijana Radic Stojkovic, Ivo Piantanida, Domagoj Eljuga, Janez Kosmrlj and Maja Osmak
.04 mL medium/well was added. Three hours later, formazan crystals were dissolved in DMSO (0.17 mL/well), the plates were mechanically agitated for 5 min and the optical density at 545 nm was determined on a microtiter plate reader (Awareness Technology Inc, Palm City, FL, USA). To examine the effect of reactive oxygen species (ROS) scavengers on survival of 2b treated cells, the same procedure was used as described above, except that two hours prior to addition of 2b, 5 mM of N-acetyl-cysteine (NAC, Sigma-Aldrich), or 1mM tempol (Santa Cruz Biotechnology, Dallas, TX
Teuta Murati, Marina Miletić, Josipa Kolarić, Vanja Lovrić, Danijela Bursać Kovačević, Predrag Putnik, Irena Landeka Jurčević, Domagoj Đikić, Verica Dragović-Uzelac and Ivana Kmetič
23. Zhu C, Hu W, Wu H, Hu X. No evident dose-response relationship between cellular ROS level and its cytotoxicity-a paradoxical issue in ROS-based cancer therapy. Sci Rep 2014;4:5029. doi: 10.1038/srep05029
24. Kong Q, Beel JA, Lillehei KO. A threshold concept for cancer therapy. Med Hypotheses 2000;55:29-35. doi: 10.1054/mehy.1999.0982
25. Maurya DK, Devasagayam TP. Antioxidant and prooxidant nature of hydroxycinnamic acid derivatives ferulic and caffeic acids. Food Chem Toxicol 2010;48:3369-73. doi: 10.1016/j.fct.2010.09.006
the association between mitochondrial DNA (mtDNA) 4977 bp deletion and HCC, we initiated a comprehensive mutational screening for this deletion in 105 patients with HCC and 69 healthy subjects. Moreover, we evaluated the reactive oxygen species (ROS) level and mtDNA copy number in those subjects carrying these deletions.
Materials and methods
Clinical Samples and DNA Extraction
Blood and tissues of 105 patients with HCC were collected at Zhuji People’s Hospital, Shaoxing, People’s Republic of China (PRC). The lesion and the nearby normal tissue from the
Harmful effects of elevated levels of catecholamines are mediated by various mechanisms, including gene transcription and formation of oxidation products. The aim of this study was to see whether the molecular mechanisms underlying the damaging action of adrenaline on DNA are mediated by reactive oxygen species (ROS). To do that, we exposed human whole blood cells to 10 μmol L-1adrenaline or 50 μmol L-1H2O2(used as positive control) that were separately pre-treated or post-treated with 500 μmol L-1of quercetin, a scavenger of free radicals. Quercetin significantly reduced DNA damage in both pre- and post-treatment protocols, which suggests that adrenaline mainly acts via the production of ROS. This mechanism is also supported by gradual lowering of adrenaline and H2O2-induced DNA damage 15, 30, 45, and 60 min after treatment. Our results clearly show that DNA repair mechanisms are rather effective against ROS-mediated DNA damage induced by adrenaline.
, 10 , 11 , 12 ). Lead activates certain enzymes by modulating gene expression or by restricting the activity of enzyme inhibitors, but its relationship has not been yet clearly established ( 3 ). Plants employ inherent defense strategies for metal detoxification whenever confronted with stressful condition. It includes restriction of metal uptake, metal ion trafficking, metal sequestration within the root, biosynthesis of osmolytes and activation of antioxidant enzymes. In order to quench ROS and to cope up, plants also developed defense mechanism by activation of
Jarosław Gocławski, Joanna Sekulska-Nalewajko and Elżbieta Kuźniak
Huang, CX-S., Liu, J-H. and Chen, X-J. (2010). Overexpression of PtrABF gene, a bZIP transcription factor isolated from Poncirus trifoliata , enhances dehydration and drought tolerance in tobacco via scavenging ROS and modulating expression of stress-responsive genes, BMC Plant Biology 10 : 1-18, paper 230, http://www.biomedcentral.com/1471-2229/10/230.
James, W.C. (1971). An illustrated series of assessment keys for plant diseases, their preparation and usage, Canadian Plant Disease Survey 51 (2): 39-65.
Kohonen, T. (1990). The