Georgiana E. Grigore, Angela Dascalescu, Mihaela Zlei, Iuliu C. Ivanov, Catalin Danaila, Tudor Petreus and Eugen Carasevici
CD4+ T cell repertoire towards a regulatory phenotype and leads to the accumulation of CD4+ forkhead box P3+ T cells. Clin Exp Immunol. 2011;166(2):154-63.
25. Junevik K, Werlenius O, Hasselblom S, Jacobsson S, Nilsson-Ehle H, Andersson PO. The expression of NKcell inhibitory receptors on cytotoxic T cells in B-cell chronic lymphocytic leukaemia (B-CLL). Ann Hematol. 2007;86(2):89-94.
26. Rivino L, Messi M, Jarrossay D, Lanzavecchia A, Sallusto F, Geginat J. Chemokine receptor expression identifies pre-T helper (Th) 1, pre-Th2, and
Pappas M. Efthymios, Mpournaka Spiridoula, Katopodis Periklis, Chardalias Andreas, Tsakas Sotiris, Eleftheriadis Theodoros, Papachristou Evangelos, Katopodis P. Konstantinos and Goumenos S. Dimitrios
Chronic Kidney Disease (CKD) is characterized by immune activation with development of chronic inflammation. However, immune deficiency also exists in CKD patients. The number and the activity of Natural Killer cells (NK-cells) are influenced by the biocompatibility of various dialysis membranes. In this study we investigated the effect of dialysis modality and membrane type on NK-cell number and on phagocytic activity of neutrophils in patients on different dialysis methods.
Sixty patients were included in the study and divided in three groups of 20 patients each. Patients on conventional hemodialysis using Low Flux membrane (cHD-LF) were included in Group I, patients on conventional dialysis using High Flux membrane (cHD-HF) were included in Group II and patients treated by on-line hemodiafiltration with High Flux polysulphone membrane (on-line HDF) were included in Group III. Native immunity was investigated using the number of NK-cells and the phagocytic activity of neutrophils.
NK-cells count was significantly lower (p<0.001) in the three groups of dialyzed patients in comparison to healthy subjects. However, no significant difference was observed in the NK-cells count among patients treated by conventional dialysis using Low or High Flux membrane and patients treated by on-line hemodiafiltration. Similarly, although the phagocytic activity of neutrophils was significantly decreased in all patients on dialysis (p<0.001), no difference related to the dialysis modality or membrane performance was observed. A strong positive correlation was recognized between parathormone blood levels and number of NK-cells (r=0.305, p<0.01).
In conclusion, an impairment of the native immunity represented by NK cell number and phagocytic activity of neutrophils is observed in patients on dialysis. Dialysis modality and membrane performance do not influence the native immunity of dialyzed patients. However, parathormone blood levels are possibly involved in the development of immune system disturbances in such patients.
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Matevz Skerget, Barbara Skopec, Darja Zontar and Peter Cernelc
marrow aplasia followed by delayed reconstitution of lymphocytes resulting in partial recovery on collection day. 11 As a consequence lower doses of lymphocytes are col lected and reinfused. There is accumulating evidence that the dose of infused lymphocytes and their recovery on day 15 and 30 after AHSCT are independent prognostic factors for overall survival in patients with non-Hodgkin lymphomas and myeloma. 12 - 15 Among lymphocyte subpopulations the CD16+/56+ natural killer (NK) cells are particularly strong independent predictors of survival. 12
Magdalena Hryhorowicz, Joanna Zeyland, Agnieszka Nowak-Terpiłowska, Jacek Jura, Wojciech Juzwa, Ryszard Słomski, Jan Bocianowski, Zdzisław Smorąg, Anna Woźniak and Daniel Lipiński
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inducing a series of immune responses within the infected organism. These immune responses are mediated by dendritic cells, natural killer (NK) cells, monocytes/macrophages, cluster of differentiation (CD)8 + T-lymphocytes, CD4 + T-lymphocytes, and other immune cells that play an important role in chronic HBV infection. Some studies have shown that in CHB patients, these immune cells have different degrees of dysfunction, but the mechanism underlying this phenomenon is poorly understood [ 3 , 4 , 5 , 6 ]. The expression of some immune molecules in these immune cells
Dorota Kostrzewa-Nowak, Rafał Buryta and Robert Nowak
, USA) and their results of were calculated using BD Accuri™ C6 software (ver. 1.0.264.21).
Lymphocyte subsets phenotyping in erythrocytelysed blood samples was performed using BD Multitest™ IMK kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol. Briefly, two antibody cocktails (designated in our laboratory as »A« and »B«, respectively) to determine the percentages of T lymphocyte subsets (»A«) as well as lymphocyte B and NKcells (»B«) in erythrocyte-lysed blood samples were used. Cocktail »A« contained antibodies including fluorescein
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Judit Beáta Köpeczi, Erzsébet Benedek, Enikő Kakucs, Aliz Tunyogi, Monica Istrati and Istvan Benedek
4(+)CD56(+) malignancies. Blood. 2002;99(5):1556-63. DOI: 10.1182/blood.V99.5.1556
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12. Petrella T, Bagot M, Willemze R, Beylot-Barry M, Vergier B, Delaunay M, et al. Blastic NK-cell lymphomas (agranular CD4+CD56+ hematodermic neoplasms): a review. Am J Clin
Aleksandar Petlichkovski, Zvonko Milenkovic, Eli Djulejic, Bisera Jefremovska, Haris Babacic, Ljubomir Ivanovski, Irena Kondova Topuzovska, Katerina Spasovska and Mirko Spiroski
T cell responses require interactions with dendritic cells in the lungs. J Exp Med. 2008;205(7):1635-46.
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