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Rai stage-related changes within T/NK cell populations from B-CLL patients

CD4+ T cell repertoire towards a regulatory phenotype and leads to the accumulation of CD4+ forkhead box P3+ T cells. Clin Exp Immunol. 2011;166(2):154-63. 25. Junevik K, Werlenius O, Hasselblom S, Jacobsson S, Nilsson-Ehle H, Andersson PO. The expression of NK cell inhibitory receptors on cytotoxic T cells in B-cell chronic lymphocytic leukaemia (B-CLL). Ann Hematol. 2007;86(2):89-94. 26. Rivino L, Messi M, Jarrossay D, Lanzavecchia A, Sallusto F, Geginat J. Chemokine receptor expression identifies pre-T helper (Th) 1, pre-Th2, and

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The Effect of Dialysis Modality and Membrane Performance on Native Immunity in Dialysis Patients


Chronic Kidney Disease (CKD) is characterized by immune activation with development of chronic inflammation. However, immune deficiency also exists in CKD patients. The number and the activity of Natural Killer cells (NK-cells) are influenced by the biocompatibility of various dialysis membranes. In this study we investigated the effect of dialysis modality and membrane type on NK-cell number and on phagocytic activity of neutrophils in patients on different dialysis methods.

Sixty patients were included in the study and divided in three groups of 20 patients each. Patients on conventional hemodialysis using Low Flux membrane (cHD-LF) were included in Group I, patients on conventional dialysis using High Flux membrane (cHD-HF) were included in Group II and patients treated by on-line hemodiafiltration with High Flux polysulphone membrane (on-line HDF) were included in Group III. Native immunity was investigated using the number of NK-cells and the phagocytic activity of neutrophils.

NK-cells count was significantly lower (p<0.001) in the three groups of dialyzed patients in comparison to healthy subjects. However, no significant difference was observed in the NK-cells count among patients treated by conventional dialysis using Low or High Flux membrane and patients treated by on-line hemodiafiltration. Similarly, although the phagocytic activity of neutrophils was significantly decreased in all patients on dialysis (p<0.001), no difference related to the dialysis modality or membrane performance was observed. A strong positive correlation was recognized between parathormone blood levels and number of NK-cells (r=0.305, p<0.01).

In conclusion, an impairment of the native immunity represented by NK cell number and phagocytic activity of neutrophils is observed in patients on dialysis. Dialysis modality and membrane performance do not influence the native immunity of dialyzed patients. However, parathormone blood levels are possibly involved in the development of immune system disturbances in such patients.

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KIR And HLA Haplotype Analysis in a Family Lacking The KIR 2DL1-2DP1 Genes

References 1. Rajalingam R. Human diversity of killer cell immunoglobulin-like receptors and disease. Korean J Hematol. 2011; 46(4): 216-228. 2. Jiang W, Johnson C, Jayaraman J, Simecek N, Noble J, Moffatt MF, et al. Copy number variation leads to considerable diversity for B but not A haplotypes of the human KIR genes encoding NK cell receptors. Genome Res. 2012; 22(10): 1845-1854. 3. Hou L, Chen M, Ng J, Hurley CK. Conserved KIR allele-level haplotypes are altered by microvariation in individuals with European

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Mobilization with cyclophosphamide reduces the number of lymphocyte subpopulations in the leukapheresis product and delays their reconstitution after autologous hematopoietic stem cell transplantation in patients with multiple myeloma

marrow aplasia followed by delayed reconstitution of lymphocytes resulting in partial recovery on collection day. 11 As a consequence lower doses of lymphocytes are col lected and reinfused. There is accumulating evidence that the dose of infused lymphocytes and their recovery on day 15 and 30 after AHSCT are independent prognostic factors for overall survival in patients with non-Hodgkin lymphomas and myeloma. 12 - 15 Among lymphocyte subpopulations the CD16+/56+ natural killer (NK) cells are particularly strong independent predictors of survival. 12 T regulatory

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Characterization of Three Generations of Transgenic Pigs Expressing the HLA-E Gene

-geneic human NK cytotoxicity. Hum. Immunol., 61: 1066–1073. Forte P., Lilienfeld B.G., Baumann B.C., Seebach J.D. (2005). Human NK cytotoxicity against porcine cells is triggered by NKp44 and NKG2D. J. Immunol., 175: 5463–5470. Forte P., Baumann B.C., Schneider M.K., Seebach J.D. (2009). HLA-Cw4 expression on porcine endothelial cells reduces cytotoxicity and adhesion mediated by CD158a + human NK cells. Xenotransplantation, 16: 19–26. Garrick D., Fiering S., Martin D.I., Whitelaw E. (1998). Repeat-induced gene silencing in mammals. Nat. Genet., 18: 56

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Immunomodulatory effects of Tim-3 and PD-1 on chronic hepatitis B virus infection

inducing a series of immune responses within the infected organism. These immune responses are mediated by dendritic cells, natural killer (NK) cells, monocytes/macrophages, cluster of differentiation (CD)8 + T-lymphocytes, CD4 + T-lymphocytes, and other immune cells that play an important role in chronic HBV infection. Some studies have shown that in CHB patients, these immune cells have different degrees of dysfunction, but the mechanism underlying this phenomenon is poorly understood [ 3 , 4 , 5 , 6 ]. The expression of some immune molecules in these immune cells

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Comparison of selected CD45+ cell subsets’ response and cytokine levels on exhaustive effort among soccer players

, USA) and their results of were calculated using BD Accuri™ C6 software (ver. Lymphocyte subsets phenotyping in erythrocytelysed blood samples was performed using BD Multitest™ IMK kit (BD Biosciences, San Jose, CA, USA) according to the manufacturer’s protocol. Briefly, two antibody cocktails (designated in our laboratory as »A« and »B«, respectively) to determine the percentages of T lymphocyte subsets (»A«) as well as lymphocyte B and NK cells (»B«) in erythrocyte-lysed blood samples were used. Cocktail »A« contained antibodies including fluorescein

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MICA Polymorphism, Association with Diseases and the Role of Anti-MICA Antibodies in Organ and Stem Cell Transplantation

America. 1996;93(22):12445-50. 7. Bauer S. Activation of NK Cells and T Cells by NKG2D, a Receptor for Stress-Inducible MICA. Science. 1999;285(5428):727-9. 8. Zwirner NW, Dole K, Stastny P. Differential surface expression of MICA by endothelial cells, fibroblasts, keratinocytes, and monocytes. Human Immunology. 1999;60(4):323-30. 9. Zwirner NW, Fernández-Viña MA, Stastny P. MICA, a new polymorphic HLA-related antigen, is expressed mainly by keratinocytes, endothelial cells, and monocytes. Immunogenetics. 1998

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Blastic plasmacytoid dendritic cell neoplasia - a rare type of acute leukemia

4(+)CD56(+) malignancies. Blood. 2002;99(5):1556-63. DOI: 10.1182/blood.V99.5.1556 11. Cota C, Vale E, Viana I, Requena L, Ferrara G, Anemona L, et al. Cutaneous manifestations of blastic plasmacytoid dendritic cell neoplasm-morphologic and phenotypic variability in a series of 33 patients. Am J Surg Pathol. 2010;34(1):75-87. DOI: 10.1097/PAS. 0b013e3181c5e26b 12. Petrella T, Bagot M, Willemze R, Beylot-Barry M, Vergier B, Delaunay M, et al. Blastic NK-cell lymphomas (agranular CD4+CD56+ hematodermic neoplasms): a review. Am J Clin

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Association of Killer Cell Immunoglobulin-Like Receptor Genes with Pandemic Influenza A (H1N1)pdm09 Infection in Critically III Macedonian Patients

T cell responses require interactions with dendritic cells in the lungs. J Exp Med. 2008;205(7):1635-46. 13. Belz GT, Bedoui S, Kupresanin F, Carbone FR, Heath WR. Minimal activation of memory CD8+ T cell by tissue-derived dendritic cells favors the stimulation of naive CD8+ T cells. Nat Immunol. 2007;8(10):1060-6. 14. Mandelboim O, Lieberman N, Lev M, Paul L, Arnon TI, Bushkin Y, Davis DM, Strominger JL, Yewdell JW, Porgador A. Recognition of haemagglutinins on virus-infected cells by NKp46 activates lysis by human NK cells. Nature

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