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Loss of P16 in Esophageal Adenocarcinoma Detected by Fluorescence in situ Hybridization and Immunohistochemistry

. 2016;7:38876-38883. 21. Bollschweiler E, Wolfgarten E, Gutschow C, Hölscher AH. Demographic variations in the rising incidence of esophageal adenocarcinoma in white males. Cancer. 2001;92:549-555. 22. Lagergren J, Lagergren P. Recent developments in esophageal adenocarcinoma. CA Cancer J Clin. 2013;63:232-248. 23. Duraiyan J, Govindarajan R, Kaliyappan K, Palanisamy M. Applications of immunohistochemistry. J Pharm Bioallied Sci. 2012;4(Suppl 2):S307-9. 24. Le Beau MM. Fluorescence in situ hybridization in cancer diagnosis. Important Adv Oncol

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The Prognostic Role of Immunohistochemistry in Primary Bone Tumors

Abstract

In the last decades, immunohistochemistry (IHC) has shown an important role in tumor differential diagnosis and has proven its prognostic role. Due to the various histological subtypes of primary malignant bone tumors, its role will be more important in the future. Based on recent studies, tumor growth, cell mobility, and metastases strongly correlate to the survival of these patients. An increased number of specific IHC markers have been used to determine the aggressiveness of the metastatic capacity of these tumors in enhancing early specific oncological therapies. This study presents 15 cases of primary malignant bone tumors, over a period of 4 years (2014-2018), from the Orthopedics and Traumatology Department of the University Emergency Hospital in Bucharest, where we evaluated the proliferation index using Ki67 IHC marker, tissue remodelation, the presence of necrosis in osteosarcoma cases in which chemotherapy was applied, and the aspect of the cytoskeleton with vimentin. Besides the histological aspect, we evaluated the tumor site, invasion and extension to the surrounding tissues (Computer Tomography, Magnetic Resonance) and vascularization with angiography, all these properties having an important prognostic role. Our findings were similar to other research papers in literature; showing that a fast growing and high proliferation index with increased cell mobility has a worse prognosis. Pulmonary metastases occurred in a relative short time in high-grade osteosarcomas, despite the chemotherapy, and multiple metastases were present at 6 months in a dedifferentiated chondrosarcoma case, in which the high-grade sarcoma was an osteosarcoma. Based on the literature, we also think that some specific markers might have multiple roles regarding the tumor growth local invasion and metastasis.

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Central retesting of breast cancer with HER2 immunohistochemistry score of 0 or 1+ using silver-enhanced in situ hybridisation: a multicentre, prospective study in Greece

S, Starczynski J, Morgan JM, Jasani B, Miller K (2009) Chromogenic in situ hybridization: a multicenter study comparing silver in situ hybridization with FISH. Am J Clin Pathol 132:514–520. doi: 10.1309/ AJCPXY3MJ6GSRCYP [19] Ross JS (2011) Point: Fluorescence in situ hybridization is the preferred approach over immunohistochemistry for determining HER2 status. Clin Chem 57:980–982. doi: 10.1373/ clinchem.2010.160762 [20] Cuadros M, Villegas R (2009) Systematic review of HER2 breast cancer testing. Appl Immunohistochem Mol Morphol 17:1–7. doi: 10.1097/PAI.0b

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Detection of HPV Positivity by Immunohistochemistry in Colposcopic Cervical Biopsies with a Cytological Diagnosis of ASCUS / ASCUS Tanısı Olan Hastaların Kolposkopik Biyopsi Örneklerinde İmmünohistokimyasal Olarak Human Papilloma Virüs Pozitifliğinin Araştırılması

Abstract

Objective: Many epidemiological studies have shown that human papillomavirus related infections play a major role in cervical preinvasive and invasive lesions. ASCUS (atypical cells of undetermined significance) observed in about 4-5% of all cervical cytology specimens. We searched for the presence of HPV with immunohistochemical methods in the biopsy material of patients diagnosed with ASCUS using cytology.

Material and Method: The colposcopic biopsy or Loop Electro Excisional Procedure (LEEP) material of 115 patients with a diagnosis of ASCUS were evaluated. HPV (type 6,11,16,18,31,33,42,51,52,56 and 58) immunohistochemistry was applied to all materials. The relationship between the biopsy results and HPV positivity was investigated.

Results: Cervical intraepithelial neoplasia (CIN) I was found in 34.8%, CIN II in 13% and CIN III in 9.6% of the patients and cervicitis was present in 42.6% of the patients. HPV immunohistochemistry was positive in 11 cases (9.6%) and no staining was seen in 104 cases (90.4%). No positive staining was seen in the chronic cervicitis cases. The rate of positive staining was 15% (6/34) in the CIN I diagnosed group, 20% (3/12) in the CIN II diagnosed group and 18.2% (2/9) in the CIN III diagnosed group.

Conclusion: HPV positivity was found to be lower compared to the literature both in total and in cases diagnosed by biopsy. No staining occurred in any patient with cervicitis. In conclusion, we believe that immunohistochemical examination is not an appropriate method for the determination of HPV.

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Increased p63 expression in canine perianal gland tumours

Perianal 31 Yorkshire Terrier SF 13 N/ A Perianal 32 Dachshund IM 10 N/ A Unk 32 Shih-Tzu IM 15 N/A Perianal (8.9kg) 33 Beagle IM 10 3 Perianal Unk – unknown, IM – intact male, CM – castrated male, IF – intact female, SF – spayed female BCS – body condition score, BW – body weight Immunohistochemistry FFPE tissues were sectioned at 4 μm, deparaffinised in xylene, hydrated in graded ethanol, and washed in phosphate-buffered saline. Endogenous peroxidase

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Brief communication (Original). Trefoil factor expression by immunohistochemistry in patients with oral lichen planus

Abstract

Background: Oral lichen planus (OLP) is a chronic immune-mediated inflammatory disease that usually causes oral mucosal damage. OLP has been considered to be a potentially malignant disorder associated with an increased risk for oral cancer. Trefoil factors (TFFs) are mainly synthesized by mucin-producing epithelial cells. Expression of TFFs in oral epithelia is thought to be an essential factor in protection against oral mucosal damage. However, very little is known about the connection between tissue damage of oral mucosa in OLP patients and expression of TFFs.

Objectives: To determine levels of TFF expression in oral mucosa from patients with OLP.

Methods: Tissue samples were collected from 35 patients with normal oral mucosa (controls) and 35 patients with OLP. An immunohistochemical method was used to determine the expression of the 3 TFFs (TFF1, 2, and 3) in mucosal tissues of the oral cavity.

Results:TFF2 and TFF3 expression in oral epithelia were significantly decreased in OLP patients (Mann- Whitney U test; P = 0.006, and P = 0.002, respectively). There were no significant differences in TFF1 expression between OLP patients and control subjects.

Conclusion: The present study demonstrated reduced expression of TFF2 and TFF3 in oral epithelia of OLP patients. These outcomes support our previous observations that chronic inflammation may play a major role in downregulation of TFF expression, which may be associated with oral mucosal damage in OLP patients.

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Tissue Pathomorphology and Immunohistochemistry in Mink (Neovison Vison) Fed Blood Plasma Supplemented Diet in the Period of Preparation for Breeding

Abstract

The research carried out at a mink farm aimed to determine the effect of blood plasma supplemented diet applied at the period preparing mink for reproduction on the animal organism. The studies included four groups of mink. The control group received a non-supplemented diet, while the experimental groups had feed with additive of 0.5%, 1.5%, and 2.5% of beef-pork plasma in the daily feed ration. The pathomorphological and immunohistochemical evaluation was performed on the liver, kidneys, lymph nodes, spleen, and bowel from all the groups. Pathomorphological and immunohistochemical changes of various intensity were observed in the examined organs from all experimental groups.

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Impact of g-Load Shift on Temporal Expression Pattern of Apoptosis-linked Proteins in the Rat Mammary Gland

both hypo-and hyper-gravity showed that changes in the inertial force affected steady-state expression of transcripts regulating circadian rhythm, post-synthetic DNA modification, and cellular mechano-transduction ( Casey et al., 2015 ). Our recent investigation employing spectral-based quantitative immunohistochemistry demonstrated that the composition of cytoskeletal proteins in the pregnant and lactating mammary glands was altered with the g -load shift ( Alula et al., 2018 ). However, there are no data on the effects of altered gravity on the expression dynamics

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Expression of Annexin A1 and UCH-L1 in central nervous system neuroepithelial tumors

, Jouvet A, et al. The 2007 WHO classification of tumors of the central nervous system. Acta Neuropathol. 2007; 114:97-109. 4. Mckeever PE. Insights about brain tumors gained through immunohistochemistry and in situ hybridization of nuclear and phenotypic markers. J Histochem Cytochem. 1998; 46:585-94. 5. Chen D, Chen F, Yang X, Lu X, Zu Z, Pan J, et al. Identification of prohibitin as a potential biomarker for colorectal carcinoma base on proteomics technology. Int J oncol. 2010; 37:355-65. 6. Wang J, Dong Y, Wang X, Ma H

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Evaluation of the prognostic benefit of identifying the probable primary site in cancer of unknown primary

://www.ncbi.nlm.nih.gov/pubmed/19531448 [33] Varadhachary GR, Abbruzzese JL, Lenzi R. Diagnostic strategies for unknown primary cancer. Cancer [Internet]. 2004 May 1 [cited 2015 Jun 1];100(9):1776-85. Available from: http://www.ncbi.nlm.nih.gov/pubmed/15112256 [34] Handorf CR, Kulkarni A, Grenert JP, Weiss LM, Rogers WM, Kim OS, et al. A multicenter study directly comparing the diagnostic accuracy of gene expression profiling and immunohistochemistry for primary site identification in metastatic tumors. Am J Surg Pathol [Internet]. 2013 Jul [cited 2015 Sep 30

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