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Antimicrobial resistance of Enterococcus faecalis isolated from meat

.A.: Enterococcus faecalis and Enterococcus faecium isolates from milk, beef and chicken and their antibiotic resistance. J Food Protect 2003, 6, 331-336. 4. Dicuonzo G., Gherardi G., Lorino G., Angelotti S., Battistoni F., Bertuccini R., Creti R., Di Rosa R., Venditti M., Baldassarri L.: Antibiotic resistance and genotypic characterization by PFGE of clinical and environmental isolates of enterococci. FEMS Microbiol Lett 2001, 201, 205-211. 5. Ducková V., Čanigová M., Kročko M., Lavová M.: Antibiotic susceptibility and biofilm-forming capacity of

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Genotypic characterization of Enterococcus species isolated from the oral cavity and their pattern of antibiotic susceptibility

Enterococci are commonly encountered and predominate oral infections; especially those associated with necrotic pulp, root canal infections, and periodontitis [ 1 , 2 ]. They comprise only a minimal proportion of the oral flora, but frequently occur as contaminants of food, such as meat and cheese [ 2 ]. Because Enterococcus are potential nosocomial oral pathogens, the emergence of multiresistant strains has increased interest in their pathogenicity and potential drug resistance [ 3 ]. Many studies have demonstrated a role of Enterococcus in causing

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The Effect of a Probiotic Containing Enterococcus faecium DSM 7134 on Redox and Biochemical Parameters in Chicken Blood

and malondialdehyde concentration of broiler chickens. Antioxidants, 2: 326–339. Arslan C. (2006). L-carnitine and its use as a feed additive in poultry feeding a review. Rev. Med. Vet., 157: 134–142. Audisio M.C., Oliver G., Apella M.C. (2000). Protective effect of Enterococcus faecium J96, a potential probiotic strain, on chicks infected with Salmonella pullorum. J. Food Protect., 63: 1333–1337. Aw T.Y., Wiliams M.W., Gray L. (1992). Absorption and lymphatic transport of peroxidized lipids by rat small intestine in vivo : role of mucosal GSH

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In vivo Model Experiment Using Laying Hens Treated with Enterococcus faecium EM41 from Ostrich Faeces and its Enterocin EM41

REFERENCES 1. Hádryová, J. (2011). Bakteriocín-produkujúce baktérie a ich využitie pre zdravie. Bacteriocin-producing bacteria and their use for health, in Slovak), Diploma thesis, University P. J. Šafárik, Faculty of Natural Science, Institute of Animal Physiology, (p.1-69). 2. Lauková, A., Hádryová, J., Imrichová, J., Strompfová, J., Kandričáková, A. (2012). Characterization of bacteriocin-producing strain Enterococcus faecium EM41, isolate from ostrich and its bacteriocin. Proceedings of Hygiena Alimentorum XXXIII.-Safety and quality of poultry

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In vitro resistance to daptomycin of staphylococcus aureus and enterococcus spp. strains


Daptomycin is the first antibiotic in a new class of cyclic lipopeptides, active in vitro against Gram - positive cocci. The mechanism of action is a rapid depolarization of the bacterial membrane potential, loss of cytoplasmic contents, mainly K+ ions and inhibition of protein, DNA and RNA synthesis, followed by the bacterial cell death. There were investigated 112 strains of Gram-positive cocci recommended for daptomycin susceptibility testing. Since the main clinical indications of daptomycin are the severe skin and soft tissue infections caused by methicillin-resistant Staphylococcus aureus, vancomicin-resistant enterococci and penicillinresistant Streptococcus spp, we tested daptomycin activity only in these species.

The susceptibility tests was performed by Kirby- Bauer disc-diffusion method on Muller-Hinton agar supplemented with Ca 50 mg / ml. (CAMH - Ca 2 + ) as recommended by CLSI.

During the period in which we conducted the study we isolated 112 strains of Gram-positive cocci, which we tested sensitivity to daptomycin. Resistance to Daptomycin of Staphylococcus aureus (4.11%) was lower than resistance to vancomycin (6.76%), but higher than the resistance to teicoplanin (2.71%) and linezolid (1.38%).

In our study we did not identify daptomycin resistant strains of Enterococcus spp. In severe infections that require more aggressive treatment using active antibiotics on MRSA, daptomycin is a valid therapeutic option, but a susceptibility test is required to ensure effective indication. There were no daptomycin resistant Enterococcus spp. strains

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The effect of addition of probiotic bacteria (Bacillus subtilis or Enterococcus faecium) or phytobiotic containing cinnamon oil to drinking water on the heath and performance of broiler chickens

poultry. Worlds Poult. Sci. J., 74: 89-100. Awad W.A., Ghareed K., Abdel-Raheem S., Böhm J. (2009). Effects of dietary inclusion of probiotic and synbiotic on growth performance, organ weights, and intestinal histomorphology of broiler chickens. Poult. Sci., 88: 49-55. Awad W.A., Ghareed K., Böhm J. (2008). Intestinal structure and function of broiler chickens on diets supplemented with a synbiotic containing Enterococcus faecium and oligosaccharides. Int. J. Mol. Sci., 9: 2205-2216. Bai K., Feng C., Jiang L., Zhang L., Zhang J., Zhang L., Wang T

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In Vitro Effects of Enterococcus Faecalis and Selected Biomolecules on the Motility of Rabbit Spermatozoa

in streptozotocin-induced diabetic rats. African Journal of Traditional, Complementary and Alternative Medicines, 11:8-15, 2014. BIELANSKI, A.: Disinfection procedures for controlling microorganisms in the semen and embryos of humans and farm animals. Theriogenology, 68:1-22, 2007. BILLSTROM, H., LUND, B., SULLIVAN, A., NORD, CE.: Virulence and antimicrobial resistance in clinical Enterococcus faecium. International Journal of Antimicrobial Agents, 32:374-377, 2008. BUCAK, MN., ATESSAHIN, A., YUCE, A.: Effect of

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Bioactive Enterococci Isolated from Slovak Ewes’ Lump Cheese

REFERENCES Alatoom AA, Cunningham SA, Ihde S, Mandrekar J, Patel R (2011): Comparison of direct colony method versus extraction method for identification of Gram-positive cocci by use of Bruker Biotyper matrix-assissted laser desorption ionization-time of flight mass spectrometry. Journal of Clinical Microbiology, 49, 2868–2873. Aymerich T, Holo H, Havarstein LS, Hugas M, Garriga M, and Nes IF (1996): Biochemical and genetic characterization of enterocin A from Enterococcus faecium , a new antilisterial bacteriocin in the pediocin family of

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Interplay Between Bacteriophages and Restriction-Modification Systems in Enterococci

References ACKERMANN, H.W., KROPINSKI, A.M.: Curated list of prokaryote viruses with fully sequenced genomes. Res. Microbiol., 158, 2007, 555-566. BICKLE, T.A., KRUGER, D.H.: Biology of DNA restriction. Microbiol. Rev., 57, 1993, 434-450. BREDE, D.A., SNIPEN, L.G., USSERY, D.W., NEDERBRAGT, A.J., NES, I.F.: Complete genome sequence of the commensal Enterococcus faecalis 62, isolated from a healthy Norwegian infant. J. Bacteriol., 193, 2011, 2377-2378. DERESINSKI, S.: Bacteriophage Therapy

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Occurrence of enterococci in mastitic cow’s milk and their antimicrobial resistance

resistances in Enterococcus faecalis and Enterococcus faecium collected from dairy and human samples in North Italy. Food Control, 2008, 19, 886–892. 7. Cervinkova D., Vlkova H., Borodacova I., Makovcova J., Babak V., Lorencova A., Vrtkova I., Marosevic D., Jaglic Z.: Prevalence of mastitis pathogens in milk from clinically healthy cows. Vet Med 2013, 58, 567–575. 8. Chajęcka-Wierzchowska W., Zadernowska A., Łaniewska-Trokenheim Ł.: Antibiotic resistance of Enterococcus strains present in food. Kosmos 2017, 314, 1, 67–79. 9. Ebrahimi A., Nikookhah F

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