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Elisa Method For Serum Hepcidin Quantification In Bulgarian Population

References 1. Andrews , N. C. Anemia of infl ammation: the cytokine-hepcidin link. - J. Clin. Invest., 113, 2004, 1251-1253. 2. Anthony, M. et al. Dual-Monoclonal Sandwich ELISA Specifi c for Hepcidin-25. - Clin. Chem., 56, 2010, № 11, 1725-1732. 3. Ashby, D. R. et al. Erythropoietin administration in humans causes a marked and prolonged reduction in circulating hepcidin. - Haematologica, 95, 2010, 505-508. 4. Ashby, D. R. et al. Plasma hepcidin levels are elevated but responsive to

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DOT-ELISA and parasitological examination for diagnosis of Schistosoma mansoni infection in Nigeria

] Attah, D. D., Dakul, D. A., Adamu, T., Uneke, C. J., Kumbak, D. (2002): Prevalence of shistosomiasis in the former Zuru emirate council, Kebbi State, Nigeria. Nig. J. Exptl. Appl. Biol., 3: 195–199 [4] Boctor, F. N., Stek, M. J., Peter, J. B., Kamal, R. (1987): Simplification and standardzation of dot-ELISA for human Schistosoma mansoni. J. Parasitol., 73: 589–592 http://dx.doi.org/10.2307/3282141 [5] Boros, D. L., Warren, K. S. (1970): Delayed hypersensitivity-type granuloma formation and dermal reaction induced

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The Use of Chicken Igy in a Double Antibody Sandwich Elisa for the Quantification of Melittin in Bee Venom and Bee Venom Melittin Content in Cosmetics

Enzymology. 184: 51-67. Gujral N., Suresh M. R., Sunwoo H. (2012a) Quantitative double antibody sandwich ELISA for the determination of gliadin. Journal of Immunoassay and Immunochemistry 33(4): 339-351. DOI: 10.1080/15321819.2012.655823 Gujral N., Lobenberg R., Suresh M., Sunwoo H. (2012b) In-Vitro and In-Vivo Binding Activity of Chicken Egg Yolk Immunoglobulin Y (IgY) against Gliadin in Food Matrix. Journal of Agricultural and Food Chemistry 60(12): 3166-3172. DOI: 10.1021/jf205319s Habermann E. (1972) Bee wasp venoms

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Seroprevalence of Fasciola hepatica infection in cattle and sheep in the province of Kars, Turkey, as determined by ELISA

diagnostic test. Int. J. Parasitol., 36, 1153–1158. DOI: 10.1016/j.ijpara.2006.06.001 http://dx.doi.org/10.1016/j.ijpara.2006.06.001 [12] Salimi-Bejestani, M. R., Mcgarry, J. W., Felstead, S., Ortiz, P., Akca, A., Williams, D. J. (2005): Development of an antibody-detection ELISA for Fasciola hepatica and its evaluation against a commercially available test. Res. Vet. Sci., 78, 177–181. DOI: 10.1016/j.rvsc.2005.08.005 http://dx.doi.org/10.1016/j.rvsc.2004.08.005 [13] Sarimehmetoglu, H.O. (2002): Application of western

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Detection of antibodies against Lumpy skin disease virus by Virus neutralization test and ELISA methods

. Comp. Immun. Microbiol Infect Dis 1983, 6, 3, 209-213. 20. Babiuk S, Bowden TR, Parkyn G, Dalman B, Manning L, Neufeld J, Embury-Hyatt C, Copps J, Boyle DB: Quantification of Lumpy Skin Disease Virus Following Experimental Infection in Cattle. Transboun Emerg Dis 2008, 55:299-307. 21. Babiuk S, Wallace DB, Smith SJ, Bowden TR, Dalman B, Parkyn G, Copps J and Boyle DB: Detection of Antibodies Against Capripoxviruses using an inactivated Sheeppox virus ELISA. Transbound Emerg Dis 2009, 56:132–141. 22. Walid SA, Adel KI, Khaled M, Khaled MF, Mervet IAM

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ELISA validation and determination of cut-off level for chloramphenicol residues in honey

Abstract

An analytical validation of a screening ELISA for detection of chloramphenicol (CAP) in honey was conducted according to the Commission Decision 2002/657/EC and Guidelines for the Validation of Screening Methods for Residues of Veterinary Medicines. The analyte was extracted from honey with a water and ethyl acetate mixture, and CAP concentrations were measured photometrically at 450 nm. The recovery rate of the analyte from spiked samples was 79%. The cut-off level of CAP in honey as the minimum recovery (0.17 units) was established. Detection capability (CCβ) was fixed at 0.25 μg kg−1. No relevant interferences between matrix effects and structurally related substances including florfenicol and thiamphenicol were observed. The ELISA method should be useful for determination of CAP residues in honey monitoring.

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Detection of circulating antigens in serum samples of mice experimentally infected with Trichinella spiralis by a sandwich ELISA based on IgY

://dx.doi.org/10.1051/parasite/20041113 [17] Gomez-Morales, M.A., Ludovisi, A., Amati, M., Cherchi, S., Pezzotti, P., Pozio, E. (2008): Validation of an enzyme-linked immunosorbent assay for diagnosis of human trichinellosis. Clin. Vaccine Immunol., 15: 1723–1729. DOI: 10.1128/CVI.00257-08 http://dx.doi.org/10.1128/CVI.00257-08 [18] Hegazy, I. H., El Mansoury, S. T., Boulos, L. M. (1996): Detection of circulating Trichinella pseudospiralis antigen by enzyme linked immunosorbant assay (ELISA). J. Egypt Soc. Parasitol. 26: 217

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Reaction of Sera from Infertile Female Patients with Fractionated Phylogenetically Conserved Ovarian Antigens Measured by Elisa: A Pilot Study

Summary

The reaction of anti-sperm antibody-positive sera from infertile women with fractionated mouse ovarian antigens was measured by enzyme-linked immunosorbent assay (ELISA). Antigens were obtained by extraction for nuclear matrix and intermediate filaments (NM-IF) producing three protein fractions – soluble, cytoskeletal and NM-IF. The results showed that sera from some infertile patients, but not control sera, react with either the soluble fraction or the NM-IF fraction. The reaction with soluble proteins was most likely directed against surface antigens, possibly aggravating the fertility problems, while the anti-NM-IF antibodies could indicate release of insoluble intracellular components by tissue damage of unknown origin.

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An Overview of Introducing Various Laboratory Tests for Diagnosis of Human Brucellosis in the Republic of Macedonia

;39:1661-4. Bricker BJ, Halling SM. Differentiation of Brucella abortus bv. 1, 2, and 4, Brucella melitensis, Brucella ovis, and Brucella suis bv. 1 by PCR. J Clin Microbiol. 1994;32(11):2660-6. Bricker BJ. PCR as a diagnostic tool for brucellosis. Vet microb. 2002;90(1-4):434-46. Taleski V. Enzyme Linked Immunosorbent Assay (ELISA) and Polymerase Chain Reaction in Diagnosis of Human Brucellosis. [In Macedonian]. Doctoral dissertation, 2001. Araj GF, Lulu AR, Khateeb MI, Haj M. Specific IgE response in

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Intestinal parasitic infections: high prevalence of Giardia intestinalis in children living in an orphanage compared with hill-tribe children as detected by microscopy and ELISA

Abstract

Background: Data regarding intestinal parasitic infections in preschool-aged children (less than 6 years old) living in an orphanage and remote mountainous areas are very limited.

Objectives: We surveyed infections in orphans and hill-tribe children.

Materials and Methods: They were studied in 2008 by stool examination (simple smear and concentration), Scotch-tape and culture (Boeck and Drbohlav’s Lock-Egg-Serum medium) techniques. The Giardia coproantigen ELISA was also performed. The risk correlation between unusual stool types and giardiasis by univariate analysis was tested.

Results: The overall infection rates in 137 orphans and in 145 hill-tribe children were 58.4% and 77.9%, respectively. Giardia intestinalis had the highest prevalence in orphans (with microscopy 28.5%, with copro-antigen ELISA 31.4%). Other pathogens included Blastocystis hominis (23.4%), Enterobius vermicularis (9.5%), and hookworm (0.7%), whereas the nonpathogens were Trichomonas hominis (19.0%), Entamoeba coli (11.7%), and Endolimax nana (2.2%). Ascaris lumbricoides had the highest prevalence (62.1%) in hill-tribe children, while Giardia intestinalis showed 7.6% with microscopy and 9.0% by ELISA. The other pathogens were E. vermicularis (25.5%), Trichuris trichiura (10.3%), B. hominis (2.8%), hookworm (1.4%), Sarcocystis hominis (1.4%) and E. histolytica (0.7%), whereas the nonpathogenic organisms were E. coli (19.3%), and E. nana (0.7%). Giardiasis stools from orphans had significantly greater cyst density than those from the hill-tribe children. The coproantigen ELISA for giardiasis demonstrated 91.4% specificity, 72.0% sensitivity, 64.3% positive predictive value, and 93.8% negative predictive value, respectively. By univariate analysis, a loose (mushy) stool type was 2.43 times likely to have Giardia cysts.

Conclusion: In large-scale epidemiological studies, a Giardia ELISA might be a useful aid for diagnosis, because conventional microscopy is time-consuming and relies on the expertise of the microscopist.

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