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Introduction Isolation of stem cells allowed rapid development of research concerning process of regeneration, creating further possibilities in clinical trials. During past few decades, stem cell therapy revolutionized the area of personalized medicine, bringing plentiful approaches introduced to replace damaged or necrotic tissues into existence [ 1 ]. From the number of resources in the human body serving respectively generation, proliferation and differentiation of new stem cells, dental pulp (DP) containing dental pulp stem cells (DPSCs) are the focus of

, Bakopoulou A, Papa P, Leyhausen G, Geurtsen W, Koidis P . Dental pulp stem cells’ secretome enhances pulp repair processes and compensates TEGDMA-induced cytotoxicity. Dent Mater , 2014; 30:405-418. 23. Bakopoulou A, Leyhausen G, Volk J, Tsiftsoglou A, Garefis P, Koidis P, Geurtsen W . Assessment of the Impact of Two Different Isolation Methods on the Osteo/Odontogenic Differentiation Potential of Human Dental Stem Cells Derived from Deciduous Teeth. Calcif Tissue Int , 2011; 88(2):130-141. 24. Aina V, Malavasi G, Fiorio PA, Munaron L, Morterra C . Zinc

multipotency for dentinogenesis, chondrogenesis, adipogenesis, and neurogenesis. Stem Cells. 2006; 24(11):2493-2503. Yu J, Deng Z, Shi J, Zhai H, Nie X, Zhuang H, Li Y, Jin Y. Differentiation of dental pulp stem cells into regular-shaped dentin-pulp complex induced by tooth germ cell conditioned medium. Tissue Engineering. 2006;12(11):3097-3105. El-Backly RM, Massoud AG, El-Badry AM, Sherif RA, Marei MK. Regeneration of dentine/pulp-like tissue using a dental pulp stem cell/poly (lactic-co-glycolic) acid scaffold construct in New Zealand white rabbits. Aust Endod J. 2008; 34

; 19:698-709. 10.1038/cr.2009.35 26. Gronthos S, Mankani M, Brahim J, Robey PG, Shi S. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl Acad Sci USA. 2000; 97:13625-30. 10.1073/pnas.240309797 27. Miura M, Gronthos S, Zhao M, Lu B, Fisher LW, Robey PG, et al. SHED: stem cells from human exfoliated deciduous teeth. Proc Natl Acad Sci USA. 2003; 100:5807-12. 10.1073/pnas.0937635100 28. Huang, AH, Chen YK, Lin LM, Shieh TY, Chan AW. Isolation and characterization of dental pulp stem cells from a supernumerary tooth. J Oral Pathol Med. 2008

, Jeng JH, Chang MC. Transforming growth factor beta 2 regulates growth and differentiation of pulp cells via ALK5/Smad2/3. J Endodont. 2008;34(4):427-32. 12. Ma DD, Gao J, Yue J, Yan WJ, Fang FC, Wu BL. Changes in Proliferation and Osteogenic Differentiation of Stem Cells from Deep Caries In Vitro. J Endodont. 2012;38(6):796-802. 13. Prescott RS, Alsanea R, Fayad MI, Johnson BR, Wenckus CS, Hao J, et al. In vivo generation of dental pulp-like tissue by using dental pulp stem cells, a collagen scaffold, and dentin matrix protein 1 after subcutaneous transplantation in

followed: adipose-derived stem cells (ASCs), articular cartilage-resident chondroprogenitor cells (ACPCs), mesenchymal stem cells (MSCs), endometrial mesenchymal stromal cells (eMSCs), amniotic epithelial cells (AECs), cancer stem cells (CSCs), trophoblast stem cells (TSCs), neural stem cells (NSCs) and dental pulp stem cells (DPSCs). In addition to using stem cells in clinical practice, it is equally important to understand their original properties, which should guide researchers and clinicians to their subsequent therapeutic use. Proper isolation will allow obtaining

differentiation potential. J Exp Med . 2004; 2:123-135. 5. Zuk PA, Zhu M, Ashjian P et al. Human adipose tissue is a source of multipotent stem cells. Mol Biol Cell. 2002; 13:4279- 95. 6. Friedenstein AJ. Osteogenic stem cells in the bone marrow. Bone Min Res. 1990;7:243-272. 7. Gronthos S, Mankani M, Brahim J, et al. Postnatal human dental pulp stem cells (DPSCs) in vitro and in vivo. Proc Natl acad Sci. 2000; 97:13625-13630. 8. Miura M, Gronthos S, Zhao M, et al. SHED: Stem cells from human exfoliated deciduous teeth. PNAS. 2003; 100: 5807-5812. 9. Gronthos S, Graves SE

, Cherman N, Boyde A. Stem cell properties of human dental pulp stem cells. J Dent Res. 2002;81(8):53-60.

trioxide aggregate. J. Endod., 39, 743, 2013. 12. Poggio C. et al.: Biocompatibility of a new pulp capping cement. Ann. Stomatol. (Roma), 2, 69, 2014. 13. Sulaiman J.M.A., Yahya M.M., Al-Ashou W.M.O.: An in-vitro scan electron microscope comparative study of dentine-Biodentine interface. J Bagh College Dentistry, 26, 42, 2014. 14. Zhirong L. et al.: Effect of Biodentine™ on the proliferation, migration and adhesion of human dental pulp stem cells. J. Dent., 42, 490, 2014.

Dentin-Pulp Complex in Regenerative Endodontics. J Endodont. 2016;42(1):47-56. 22. Zhu Q, Tian G, Tang Z, Gao J, Tan Y. Adrenomedullin Promotes the Proliferation and Inhibits Apoptosis of Dental Pulp Stem Cells Involved in Divergence Pathways. J Endod. 2016;42(9):1347-54. 23. Yoshida S, Wada N, Hasegawa D, Miyaji H, Mitarai H, Tomokiyo A, et al. Semaphorin 3A Induces Odontoblastic Phenotype in Dental Pulp Stem Cells. Journal of Dental Research. 2016;95(11):1282-90. 24. Choung HW, Lee DS, Lee JH, Shon WJ, Lee JH, Ku Y, et al. Tertiary Dentin Formation after Indirect