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Reproductive Disorders in Streptozotocin-Treated Male Rats Following Co-Administration of Ethambutol, Rifampicin, Isoniazid and Pyrazinamide

. Takasu N, Komiya I, Asawa T, Nagasawa Y, Yamada T. Streptozotocin- and alloxan-induced H 2 O 2 generation and DNA fragmentation in pancreatic islets. H 2 O 2 as mediator for DNA fragmentation. Diabetes 40: 1141-1145, 1991. 40. Bolzan AD, Bianchi MS. Genotoxicity of streptozotocin. Mutat Res 512: 121-134, 2002. 41. Bhide SV, Bhalerao EB, Sarode AV, Maru GB. Mutagenicity and carcinogenicity of mono- and diacetyl hydrazine. Cancer Lett 23: 235-240, 1984. 42. Braun R, Jakel HP, Schoneich J. Genetic effects of

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Damage of testicular cell macromolecules and reproductive capacity of male rats following co-administration of ethambutol, rifampicin, isoniazid and pyrazinamide

. (2005). Oxidation of ethanol to acetaldehyde and free radicals by rat testicular microsomes. Arch Toxicol   79 (1): 25-30. Ray SD, Kamendulis LM, Gurule MW et al. (1993). Ca2+ antagonists inhibit DNA fragmentation and toxic cell death induced by acetaminophen. FASEB J   7 (5): 453-463. Sanocka D, Kurpisz M. (2004). Reactive oxygen species and sperm cells. Reproductive Biology and Endocrinology   2 : 12-26. Sedlak J, Lindsay R. (1968). Estimation of total, protein-bound and nonprotein

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Sperm DNA Integrity Test and Assisted Reproductive Technology (Art) Outcome

of DNA integrity, DNA denaturation and DNA fragmentation, in fertile and infertile men. Fertil Steril. 2001;75:674-7. 16. Tomlinson MJ, MoffattO, Manicardi GC, Bizzaro D, AfnanM, Sakkas D. Interrelationshipsbetween seminal parameters and sperm nuclear DNA damage before and after density gradient centrifugation: implications for assisted conception. Hum Reprod. 2001; 16:2160-5. 17. World Health Organization. WHO laboratory manual for the examination and processing of human semen. 5th ed. Geneva: World Health Organization; 2010

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Relationship Between Apoptotic-Like Changes in Stored Boar Semen and DNA Fragmentation in Preimplantation Embryos

in vitro -derived pig expanded blastocysts based on DNA fragmentation. Anim. Sci. Pap. Rep., 27: 59-68. Chen Z., Hauser R., Trbovich A. M., Shifren J. L., Dorer D. J., Godfrey-Bailey L., Singh N. P. (2006). The relationship between human semen characteristics and sperm apoptosis: a pilot study. J. Androl., 27: 112-120. Evenson D. P. (1999). Flow cytometry assays of male fertility. Method. Cell Biol., 33: 401-410. Gjørret J. O., Knijn H. M., Dieleman S. J., Avery B., Larsson L-I., Maddox

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Effect of amifostine on sperm DNA fragmentation and testes after radioiodine treatment

gonadotropin levels in infertile males with hyperthyroidism. Fertil Steril 1976, 27, 662–666. 6. Esquerré-Lamare C., Isus F., Moinard N., Bujan L.: Sperm DNA fragmentation after radioiodine treatment for differentiated thyroid cancer. Basic Clin Androl 2015, doi: 10.1186/s12610-015-0024-1. 7. Etebari M., Jafarian-Dehkordi A., Lame V.: Evaluation of protective effect of amifostine on dacarbazine induced genotoxicity. Res Pharm Sci 2005, 10, 68–74. 8. Evan G., Littlewood T.: A matter of life and cell death. Science 1998, 281, 1317–1321. 9. Franca L

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Chronic alcoholism-mediated metabolic disorders in albino rat testes

29: 245-254. Zhang H, LeCulyse E, Liu L, Hu M, Matoney L, Zhu W, Yan B. (1999). Rat pregnane X receptor: molecular cloning, tissue distribution, and xenobiotic regulation. Arch Biochem Biophys 368: 14-22. Zhang HY, Han DW, Wang XG, Zhao YC, Zhou X, Zhao HZ. (2005). Experimental study on the role of endotoxin in the development of hepatopulmonary syndrome. World J Gastroenterol 11: 567-572. Zhivotosky B, Orrenius S. (2001). Detection of DNA Fragmentation in Whole Cells. In Current Protocols in Cell Biology. John Wiley

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Evaluation of the Genotoxic Effect of the Commercial Fungicide Tango® Super on Bovine Lymphocytes

Abstract

This study investigated the potential genotoxic effects of the fungicide Tango® Super using methods of conventional cytogenetic analysis, fluorescence in situ hybridization (FISH) and detection of DNA fragmentation in bovine lymphocytes. After exposure of two donor cell cultures to several concentrations of fungicide (0.5, 3.0 and 15.0 mg.ml-1 for conventional cytogenetic analysis; 0.5 and 3.0 mg.ml-1 for FISH) we detected the insignificant occurrence of chromosome and chromatid breakages. In both donors we observed a significant decrease in mitotic index (MI) percentage with increasing concentrations of fungicide (P < 0.01; P < 0.001), which indicated a cytotoxic effect of the preparation. Electrophoretic analysis of DNA fragmentation in lymphocytes exposed to increasing concentrations (0.5; 1.5; 3.0; 6.0 and 15.0 mg.ml-1) of this preparation showed its ability to induce formation of fragments, which is a characteristic manifestation of the last stage of apoptosis.

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Biomolecular changes and cortical neurodegenerative lesions in Trichinella spiralis infected BALB/c mice: a preliminary study elucidating a potential relationship between systemic helminthic infections and idiopathic Parkinson’s

Abstract

Idiopathic Parkinson’s (IP) is a neurodegenerative disease that is suspected to be due to exposure to infections during early life. Toxoplasmosishas been the only suspected parasitic infection in IP (Celik et al., 2010). Recently, some non-central nervous system bacterial and viral infections have been incriminated in IP (Çamcı & Oğuz, 2016). So in the current study, we tried to explore if the systemic infl ammatory reactions triggered by some helminths like Trichinella spiralis can induce Parkinsonian lesions in the brain, especially that the cerebral complications have been reported in 10-20% of Trichinella spiralis infected patients . An experimental study was designed to assess the neurodegenerative and biomolecular changes that may occur in Trichinella spiralis infected BALB/C mice in comparison to rotenone induced PD model and apparently healthy ones. The motor affection was significantly lesser in the Trichinella infected mice than the Parkinson’s model, but when the catalepsy score was calculated (through the grid and bar tests) it was found to be significantly higher in the infected mice than in the healthy ones. A significant increase in the blood advanced oxidative protein products (AOPP), IFN-γ, TGF-β, and brain DNA fragmentation was also detected in the Trichinella spiralis infected mice. After histopathological examination, a significant increase in the cortical apoptotic neurons and Lewy’s body were observed in the Trichinella infected and the rotenone induced Parkinson’s model sections. A significant decrease in the immunohistochemical expression of the tyrosine hydroxylase expression in the brain sections and the ELISA measured dopamine level in the brain homogenate was also reported in the infected mice group. This study findings may collectively suggest that the systemic inflammatory reactions and the oxidative stresses associated with some systemic helminthic infections like trichinellosis are possible to precipitate neurodegenerative lesions and biomolecular changes in the brain , and manifest with IPD later in life.

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Cytotoxicity and genotoxicity of GO-Fe3O4 hybrid in cultured mammalian cells

Abstract

The study was aimed at investigating the effect of the Fe3O4 hybrid deposited on graphene oxide (GO-Fe3O4) on the relative viability and DNA integrity. The properties of the GO-Fe3O4 hybrid were analyzed using a transmission electron microscopy (TEM), X-ray diffraction technique (XRD) and thermal gravimetric method (TGA), while the efficiency of graphene oxide covalent functionalization with iron oxide nanospheres was determined by Fourier transform infrared spectroscopy (FT-IR). L929 and MCF-7 cell lines were selected to analyze the biocompatibility of GO-Fe3O4 nanoparticles. The hybrid was tested using WST-1 and LDH leakage assays. DNA integrity was analyzed by agarose gel electrophoresis and micronucleus assay was performed to examine chromosomal damage in the exposed cell lines. The tested GO-Fe3O4 hybrid did not significantly reduce cell metabolism of L929 cells. GO-Fe3O4 hybrid particles only slightly affected the integrity of cell membranes. DNA integrity and micronucleus assays did not indicate genotoxicity of the hybrid.

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Age Dependent Effects of Metformin in Wistar Albino Male Rats with Metabolic Syndrome

. Costa LG, Hodgson E, Lawrence DA, Reed DJ, Greenlee WF. Unit 2.2. Determination of Apoptosis and Necrosis. Alternative Protocol 2. Detection of DNA fragmentation in Total Genomic DNA. In: Current Protocols in Toxicology . Costa LG, Hodgson E, Lawrence DA, Reed DJ, Greenlee WF (eds), John Wiley & Sons Inc. Somerset, pp 2759, 2005. 15. Kovalenko VM, Bagnyukova TV, Sergienko OV et al. Epigenetic changes in the rat livers induced by pyrazinamide treatment. Toxicology and Applied Pharmacology 225: 293-299, 2007 16. Ni S, Wang X, Wang J, Zhao Z, Zeng S. The

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