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Cathepsin H indirectly regulates morphogenetic protein-4 (BMP-4) in various human cell lines

10 target involved in Bmp4 degradation during lung branching morphogenesis. J Biol Chem 2007; 282 : 22176-84. Hogan BL. Bone morphogenetic proteins: multifunctional regulators of vertebrate development. Genes Dev 1996; 10 : 1580-94. Deng H, Ravikumar TS, Yang WL. Overexpression of bone morphogenetic protein 4 enhances the invasiveness of Smad4-deficient human colorectal cancer cells. Cancer Lett 2009; 281 : 220-31. Maegdefrau U, Amann T, Winklmeier A, Braig S, Schubert T, Weiss

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Differentiation of bone marrow derived mesenchymal stem cells into male germ-like cells in co-culture with testicular cells


Objective. Stem cell therapy, specifically, pre-induction of mesenchymal stem cells toward male germ-like cells may be useful in patients with azoospermia. The aim of this study was to evaluate in vitro differentiation of mouse bone marrow-derived mesenchymal stem cells (BMSCs) into male germ-like cells by indirect co-culture with testicular cells in the presence of bone morphogenetic protein 4 (BMP4).

Methods. Experimental groups included: control (mouse BMSCs), treatment group-1 (BMSCs treated with BMP4), treatment group-2 (indirect co-culture of BMSCs with mouse testicular cells in the presence of BMP4) and treatment group-3 (indirect co-culture of BMSCs with testicular cells). BMSCs-derived male germ-like cells were evaluated by the expression of Dazl, and Stra8 using RT-qPCR.

Results. Stra8 gene expression was significantly increased in the treatment group-2 and Dazl gene was significantly increased in the treatment group-1 compared to other groups. In conclusion, indirect co-culturing of BMSCs with testicular cells and BMP4 leads to the differentiation of BMSCs into male germ-like cells which express specific male germ-like genes. Testicular cells released factors that contributed to the differentiation of BMSCs into male germ progenitor cells.

Conclusion. This study suggests that mesenchymal stem cells may be differentiated into male germ-like cells and therefore, may be a novel treatment option for men with azoospermia.

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Potential of bone scaffolds containing vancomycin and bone morphogenetic protein-2 in a rat model of osteomyelitis

antibiotic use in open fractures: an evidence-based guideline. Surg Infect (Larchmt). 2006; 7:379-405. 19. Ahn SH, Kim CS, Suk HJ, Lee YJ, Choi SH, Chai JK, et al. Effect of recombinant human bone morphogenetic protein-4 with carriers in rat calvarial defects. J Periodontol. 2003; 74:787-97. 20. Hollinger JO, Schmitt JM, Buck DC, Shannon R, Joh SP, Zegzula HD, et al. Recombinant human bone morphogenetic protein-2 and collagen for bone regeneration, J Biomed Mater Res. 1998; 43:356-64. 21. Hong L, Tabata Y, Yamamoto M

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RhBMP-2 and -7 combined with absorbable collagen sponge carrier enhance ectopic bone formation: An in vivo bioassay

recombinant human BMP-2 produced in a bacterial expression system. Int J Oral Maxillofac Surg. 1998; 27: 305-9. 16. Ten Dijke P, Yamashita H, Sampath TK, Reddi AH, Estevez M, Riddle DL, et al. Identification of type I receptors for osteogenic protein-1 and bone morphogenetic protein-4. J Biol Chem. 1994; 269: 16985-8. 17. Ten Dijke P, Yamashita H, Ichijo H, Franzen P, Laiho M, Miyazono K, et al. Characterization of type I receptors for transforming growth factor-β and activin. Science. 1994; 264:101-4. 18. Lyons KM, Hogan BL

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Osteoinductive potential of small intestinal submucosa/ demineralized bone matrix as composite scaffolds for bone tissue engineering

. Clin Orthop Relat Res. 1999; 367S:S333-43. 19. Zhang M, Powers RM Jr, Wolfinbarger L Jr. Effect(s) of the demineralization process on the osteoinductivity of demineralized bone matrix. J Periodontol. 1997; 68: 1085-92. 20. Wolfinbarger L Jr, Zheng Y. An in vitro bioassay to assess biological activity in demineralized bone. In Vitro Cell Dev Biol Anim. 1993; 29A:914-6. 21. Honsawek S, Dhitiseith D. Content of bone morphogenetic protein-4 in human demineralized bone: relationship to donor age and ability to induce new

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Epithelium morphogenesis and oviduct development are regulated by significant increase of expression of genes after long-term in vitro primary culture – a microarray assays

S. Analysis of spatial and temporal expression patterns of bone morphogenetic protein family members in the rat uterus over the estrous cycle. J Endocrinol. 2004;182(2):203-17. 22. von Schalburg KR, McCarthy SP, Rise ML, Hutson JC, Davidson WS, Koop BF. Expression of morphogenic genes in mature ovarian and testicular tissues: potential stem-cell niche markers and patterning factors. Mol Reeprod Dev. 2006;73(2):142-52. 23. Abir R, Ben-Haroush A, Melamed N, Felz C, Krissi H, Fisch B. Expression of bone morphogenetic proteins 4 and 7 and their receptors

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Heat stress-induced PPAR-β enhances HUVEC resistance to oxidant injury

J Mol Med. 2009; 24: 387-92. 19. Takasu T, Lyons JC, Park HJ, Song CW. Apoptosis and perturbation of cell cycle progression in an acidic environment after hyperthermia. Cancer Res. 1998; 58: 2504-8. 20. Deng H, Ravikumar TS, Yang WL. Bone morphogenetic protein-4 inhibits heat-induced apoptosis by modulating MAPK pathways in human colon cancer HCT116 cells. Cancer Lett. 2007; 256:207-17. 21. Martindale JL, Holbrook NJ. Cellular response to oxidative stress: signaling for suicide and survival. J Cell Physiol. 2002; 192

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Assessment of noggin level in pulmonary arterial hypertension patients

. Calcium transporters and signalling in smooth muscles. Cell Calcium. 2007;42(4-5):467-76. 47. Lu W, Ran P, Zhang D, Lai N, Zhong N, Wang J. Bone morphogenetic protein 4 enhances canonical transient receptor potential expression, store-operated Ca2+ entry, and basal [Ca2+]i in rat distal pulmonary arterial smooth muscle cells. Am J Physiol Cell Physiol. 2010;299(6): C1370-78. 48. Ashwell M, Gibson S. Waist-to-height ratio as an indicator of early health risk: Simpler and more predictive than using a matrix based on BMI and waist circumference. BMJ Open. 2016

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Estimation of Endostatin level in pulmonary arterial hypertension patients and its relation with some parameters

ABBREVIATIONS ANOVA – Analysis of variance BMI – Body mass index BMP4 – Bone Morphogenetic protein-4 BMPR2 – Bone Morphogenetic Protein Receptor II CHD- Congenital Heart Disease COPD – Chronic obstructive pulmonary disease CYP1B1 – Cytochrome P450 1B1 DS – Down syndrome ES – Endostatin ESR1/Erα – Estrogen Receptor1/Estrogen Receptor Alpha HER2 – Human Epidermal growth factor Receptor-2 IPAH – Idiopathic pulmonary arterial hyper-tension LHD- Left heart disease MCTD- Mixed connective tissue disease MMP

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