Search Results

1 - 10 of 128 items :

  • Microbiology and Virology x
  • Life Sciences x
Clear All
Electrochemical reduction of azo dyes mimicking their biotransformation to more toxic products

aim of this study was to investigate the reduction products of selected azo dyes using the technique of electrochemistry with mass spectrometry (EC–MS). Afterwards, a comparison was made with data available in literature and software for predicting metabolic pathways. Particular attention was paid to the harmful aromatic amine reduction EC products. Based on these results an analytical method using liquid chromatography combined with tandem mass spectrometry (LC–MS/MS) was developed as a tool for the confirmation of the identified products obtained by

Open access
Highly Pathogenic Avian Influenza H5N1 (HPAI/H5N1) Virus Search from Wild Birds in Ghana

://www.usda.gov/wps/portal/usda/usdahome?contentid=avian_influenza.html . 17. WHO Interim Global Epidemiological Surveillance Standards for Influenza, 2012: WHO Global Technical Consultation: Global Standards and Tools for Influenza Surveillance . M http://www.who.int/influenza/resources/documents/technical_consultation/en/index.html . 18. World Organisation for Animal Health, 2016: Update on Avian Influenza in Animals (types H5 and H7) . Retrieved from http://www.oie.int/animal-health-in-the-world/update-on-avian-influenza/2016/ .

Open access
Hemolysis has no influence on routine coagulation tests in subjects without anticoagulant therapy - a referral Romanian emergency hospital laboratory experience

, Manohar C. Discrepancy in optical & mechanical method in coagulation tests in a turbid sample. Indian J Hematol Blood Transfus [Internet]. 2014/08/17. 2014 Sep;30(Suppl 1):402-4. DOI: 10.1007/s12288-014-0438-5 10. Adiga U, Yogish S. Hemolytic index-A tool to measure hemolysis in vitro. IOSR J Biotechnol Biochem (IOSR-JBB [Internet]. 2016;2(2):49-52. 11. Luoma J, Seago J, Bates T, Campbell D. Reduced Tuarnaround Time for Haemolysis/Icterus/Lipaemia (HIL) Interferent Indices on Architect chemistry analyzers, AACC Annual Meeting, 2007 12. NCCLS, How to

Open access
The influence of hemodialysis on FibroTest parameters

References 1. Poux JM, Cadranel JF, Fievet P, Dumouchel P, Collot G. Measurement of gamma glutamyl transpeptidase activity: A useful and low-cost tool for the detection of HCV infection in haemodialysed patients. Nephrol Dial Transplant. 1998;13(5):1324. DOI: 10.1093/oxfordjournals.ndt.a027886 2. Pereira BJ, Levery AS. Hepatitis C virus infection in dialysis and renal transplantation. Kidney Int. 1997;51(4):981-999. DOI: 10.1038/ki.1997.139 3. Weber D, Rutala W, Fried M. Hepatitis C Virus Outbreaks in Hemodialysis Centers: A Continuing Problem

Open access
Bivagina pagrosomi Murray (1931) (Monogenea: Polyopisthocotylea), a microcotylid infecting the gills of the gilt-head sea bream Sparus aurata (Sparidae) from the Red Sea: morphology and phylogeny

online Tool (BLAST, https://blast.ncbi.nlm.nih.gov/Blast.cgi) The sequence trimming for the congeneric species recovered was carried out by Bioedit v7.2.5, sequence alignment was done by CLUSTAL W v2.0 ( 13 ) and the phylogenetic tree was constructed using theMEGA7 ( 11 ) programme based on maximum likelihood analysis. Allodiscocotyla diacanthi ( 26 ) was employed as an out-group during tree construction. Results Diagnosis. Diagnosis was based on seven specimens. Body elongation ( Fig. 1a) was dorsoventrally flattened and lanceolated. Terminal anchors were

Open access
Review on the laboratory diagnosis of Mycoplasma pneumoniae infection

81.8% and the specificity is 98.6%. When the IgM antibody titer in the acute phase changes, the sensitivity of real-time quantitative PCR is 91.7%. Thus, real-time quantitative PCR can be used as a diagnostic tool for MP infection [ 16 ]. Other studies considered specimen collection time to have an impact on real-time quantitative PCR results. As the disease worsens, the sensitivity of PCR detected by real-time quantitative PCR also decreases [ 17 ]. 3.1.3 LAMP technology LAMP is a new technology developed in recent years. It has a high sensitivity and

Open access
Marine tetrodotoxin as a risk for human health – a review

analysing TTX and many TTX analogues such as 4-epiTTX, 11-oxoTTX, and 4,9-anhydroTTX ( 4 , 28 , 34 , 35 , 46 ). However, it has the complication of quite large differences in intensity of fluorescence between analogues, e.g . 6-epi TTX is 20 times more fluorescent than TTX, while 11-deoxy TTX is 100 times less fluorescent ( 53 ). This problem is solved when the LC– MS technique for TTX determination is used. LC–MS techniques using atmospheric pressure ionisation with an electrospray-ionisation (ESI) in positive ion mode have become a powerful tool for TTX

Open access
Evaluation of direct metagenomics and target enriched approaches for high-throughput sequencing of field rabies viruses

coverage, but with the risks of genome modification and artificial diversity caused by PCR amplification. Complete viral sequences with sufficient coverage provide the ability to discriminate between isolates that are very closely related both genetically and geographically. The application of such a powerful tool in rabies cases is crucial for better understanding of the outbreak as well as for implementing more effective rabies control strategies. In conclusion, the study describes the comparison of two approaches to the HTS of field rabies viruses. The crucial

Open access
Determination of steroid esters in hair of slaughter animals by liquid chromatography with tandem mass spectrometry

capabilities were determined. The calculation tool was ResVal software (v. 2.0) shared by the EURL in Wageningen in the Netherlands, and this application assumes the execution of four experiments ( 2 ). The instrumental linearity of the method was evaluated on the basis of four calibration curves of standard working solutions of steroid esters, prepared one for each of the four experiments assumed. All the calibration curves had seven cross points for analytes concentrations corresponding to 0, 2.5, 5.0, 7.5, 10, 25, and 50 μg kg −1 and for IS solutions at 50 μg kg −1 in

Open access
Spectrophotometric Analysis of the Resazurin Reduction Test as a Tool for Assessing Canine Semen Quality

Abstract

The resazurin reduction test (RRT) was subjected to spectrophotometric analysis to evaluate the quality of canine semen. Twenty four samples of canine semen were analysed. The absorption peaks for resazurin and resorufin were determined at 615 and 580 nm, respectively. The RRT ratio (RRTsperm-the ratio for samples containing spermatozoa, RRTplasma-the ratio for samples containing seminal plasma) was calculated by dividing the absorbance at 580 nm by the absorbance at 615 nm. Spearman’s correlation test was used to determine the significance of correlations between the analysed sperm parameters and the results of the resazurin reduction assay. The RRT ratio was highly correlated with sperm motility (r=0.68, P<0.01), progressive sperm motility (r=0.61, P<0.01), the subpopulation of cells with rapid velocity (r=0.72, P<0.01), and the subpopulation of cells with medium velocity (r= -0.54, P<0.05). A negative correlation was observed between the reducing capacity of seminal plasma vs. sperm with plasma membrane integrity (r= -0.60, P<0.01) and sperm with normal morphology (r= -0.58, P<0.01). The RRT test can be used as an additional tool for evaluation of the quality of canine semen.

Open access