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Znaczenie techniki aCGH w nowotworach mieloproliferacyjnych – przegląd literatury

. Cytogenetics and genetics of human cancer: methods and accomplishments. Cancer Genet Cytogenet 2010;203:102–126. 10.1016/j.cancergencyto.2010.10.004 21156223 Sandberg AA Meloni-Ehrig AM Cytogenetics and genetics of human cancer: methods and accomplishments Cancer Genet Cytogenet 2010 203 102 126 [58] Costa JL, Meijer G, Ylstra B, Caldas C. Array comparative genomic hybridization copy number profiling: a new tool for translational research in solid malignancies. Semin Radiat Oncol 2008;18:98–104. 10.1016/j.semradonc.2007.10.005 18314064 Costa JL

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Limfadenopatia u dzieci i dorosłych: zasady postępowania diagnostycznego

diagnostic tools. Iran J Med Sci 2014;39:158-70. 24753638 Mohseni S Shojaiefard A Khorgami Z Alinejad S Ghorbani A Ghafouri A Peripheral lymphadenopathy: approach and diagnostic tools Iran J Med Sci 2014 39 158 70 [3] Pawelec K, Wiechecka J, Boruczkowski D. Diagnostyka różnicowa powiększonych węzłów chłonnych u dzieci. Nowa Pediatr 2012;3:55-60. Pawelec K Wiechecka J Boruczkowski D Diagnostyka różnicowa powiększonych węzłów chłonnych u dzieci Nowa Pediatr 2012 3 55 60 [4] Bazemore AW, Smucker DR. Lymphadenopathy and

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Concerns about the use of ecosystem services as a tool for nature conservation: From misleading concepts to providing a “price” for nature, but not a “value”

agricultural watershed. Proc. Natl. Acad. Sci. U. S. A., 110, 12149-54. Raudsepp-Hearne, C., Peterson, G.D. & Bennett, E.M. (2010) Ecosystem service bundles for analyzing tradeoffs in diverse landscapes. Proc. Natl. Acad. Sci. U. S. A., 107, 5242-5247. Rodríguez-Loinaz, G., Alday, J.G. & Onaindia, M. (2015) Multiple ecosystem services landscape index: A tool for multifunctional landscapes conservation. J. Environ. Manage. 147, 152-163. Schröter, M., van der Zanden, E.H., van Oudenhoven, A.P.E., Remme, R.P., Serna-Chavez, H.M., de Groot, R.S. & Opdam, P. (2014) Ecosystem

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From primer design to validation of results - is it possible by using free software only? / De la proiectarea primerilor la validarea rezultatelor - este posibil utilizând doar programe gratuite?

References 1. PrimerBank [Internet]. [cited 2015 May 6]. Available from: http://pga.mgh.harvard.edu/primerbank/ 2. RTPrimerDB: the real-time PCR primer and probe database - Homepage [Internet]. [cited 2015 May 6]. Available from: http://medgen.ugent.be/rtprimerdb/index. php 3. qPrimerDepot [Internet]. [cited 2015 May 6]. Available from: http://primerdepot.nci.nih.gov/ 4. Primer designing tool [Internet]. [cited 2015 May 6]. Available from: http://www.ncbi.nlm.nih.gov/tools

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Does using the ecosystem services concept provoke the risk of assigning virtual prices instead of real values to nature? Some reflections on the benefit of ecosystem services for planning and policy consulting

-253. Martín-López, B., Gómez-Baggethun, E., García-Llorente, M. & Montes, C. (2014) Trade-offs across value-domains in ES assessment. Ecological Indicators, 37, 220-228. Morelli, F. & Moller, A.P. (2015) Concerns about the use of ES as a tool for nature conservation: From misleading concepts to providing a “price” for nature, but not a “value”. European Journal of Ecology, 1(1), 68-70. Polishchuk, Y. & Rauschmayer, F. (2012) Beyond “benefits”? Looking at ecosystem services through the capability approach. Ecological Economics, 81, 103

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Identification of Exonic Copy Number Variations in Dystrophin Gene Using Mlpa / Identificarea Variaţiilor Numărului de Copii în Gena Distrofinei Folosind Metoda Mlpa

clinical cohort. Am J Med Genet A. 2005;134(3):295-8. DOI: 10.1002/ajmg.a.30617 18. Dolinsky LC, de Moura-Neto RS, Falcao-Conceicao DN. DGGE analysis as a tool to identify point mutations, de novo mutations and carriers of the dystrophin gene. Neuromuscul Disord. 2002;12(9):845-8. DOI: 10.1016/S0960-8966(02)00069-X 19. Takeshima Y, Yagi M, Okizuka Y, Awano H, Zhang Z, Yamauchi Y, et al. Mutation spectrum of the dystrophin gene in 442 Duchenne/Becker muscular dystrophy cases from one Japanese referral center. J Hum Genet. 2010

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Diagnostic accuracy of red blood cell distribution width-to-lymphocyte ratio for celiac disease

Abstract

Background. Celiac disease (CD) is significantly underdiagnosed, despite significant efforts made in the last decades to increase its diagnostic rate. This has lead to a high need for developing new diagnostic strategies. Our aim was to evaluate the diagnostic performance of two routine hematologic indices for CD. Methods. In a prospective observational study, 34 newly diagnosed CD patients, 34 age-sex matched controls with irritable bowel syndrome (IBS) and 16 treated CD patients were assessed regarding the differences in mean lymphocyte count (LY), red blood cell distribution width (RDW) and their ratio (RDW/LY). Results. Elevated RDW (>14) and lymphopenia (<1.5 x 10e9/L) were more frequently seen in newly diagnosed CD patients compared to IBS control group and treated CD patients. Newly diagnosed CD patients had significantly higher mean values of RDW/LY - 10.09, compared to 7.72 in the CD-treated group and 6.79 in IBS controls (p<0.01). Subgroup analysis revealed that RDW/LY was higher in patients with destructive histology (Marsh≥3a), 10.54 vs. 7.99. For a value over 7, RDW/LY had a sensitivity of 88.24% (95% CI 72.55-96.70%) and AUROC of 0.785 (95% CI 0.683- 0.887). Conclusions. RDW/LY ratio is a widely available tool which could be used routinely in clinical practice for CD screening.

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Ischemic modified albumin increases in acute kidney injury

Abstract

Purpose: Acute kidney injury (AKI) is a severe kidney disease carrying high morbidity and mortality. An ischemic process, at the cellular level, has been detected prior to the full-blown AKI. An elevated ischemic modified albumin (IMA) was also found to be increased fast at several minutes following an ischemic process in the body. In this connection, we have investigated, in advance, the changes of IMA concentrations in patients with possible AKI. Methods: IMA and other biochemical and haematological parameters were measured in sera of thirty nine patients with AKI and of thirty eight healthy controls. AKI is defined by an increase in serum creatinine by ≥ 0.3 mg/dl in 48 hours or an increase by ≥ 1.5-fold from a known or assumed baseline. The results in the two groups were compared. Results: IMA, creatinine, blood urea nitrogen, white blood cell, neutrophil, neutrophil-lymphocyte ratio (NLR), platelet-lymphocyte ratio (PLR) and mean platelet volume were found to be higher in patients with AKI than in healthy controls. In contrast, total protein, albumin, lymphocyte, and haemoglobin were lower in patients with AKI than in healthy controls. No significant difference was recorded in platelet counts between the two groups. Conclusion: Our results indicate that increased levels of NLR and PLR play a central role in a systemic inflammation in AKI. Monitoring serum IMA could be a useful tool in the assessment of AKI.

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Habitat Suitability Analysis for Mountain Lions (Puma Concolor) Recolonization/ Reintroduction in Minnesota

Abstract

The mountain lion range once extended throughout the state of Minnesota. The breeding population has been greatly reduced with time, new roads, and timber harvesting, which have broken large tracts of contiguous forest into isolated patches that are too small and no longer suitable for the breeding mountain lion population. The objective of this study is to use suitability analysis to determine the most suitable habitat to conserve mountain lion populations threatened by habitat fragmentation. To attain our objective, we created three sub models that contribute to the overarching goal of the suitability model. A habitat sub model was developed for finding the best habitat, a food sub model for access to the maximum amount of food needed, and a security sub model focusing on the distance from houses, roads, and urban development. Using the Weighted Sum tool, the three sub models were combined to produce a suitability surface based on the trade-off of the preferences of the goals represented by each sub model. Our suitability model shows large areas of high-quality mountain lion habitat in the northern and north-eastern sections of the state. These areas contain favourable locations for mountain lion habitat, such as forested land cover, low-density populations, steep slopes, short distances to streams, and area unimpeded by major roads. The southern and western parts of the state are characterized by lower slopes, more agricultural land, grassland, developed land, and higher population density, which results in lower quality habitat. The twin cities have the worst mountain lion habitat.

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Phenotypic and genotypic evaluation of adherence and biofilm development in Candida albicans respiratory tract isolates from hospitalized patients

Abstract

In recent years, a significant number of epidemiological variations have been observed for fungal infections. In immunocompromised patients, Candida albicans is crucially involved in invasive infections, mostly originating in respiratory tract colonization. The global rise in candidiasis has led researchers to investigate possible correlations between fungal strains virulence profiles and their pathogenic potential, among the most investigated genes being those involved in adherence and biofilm development. In this study, we established the adherence gene profiles of C. albicans strains isolated from respiratory tract secretions in patients hospitalized for cardiovascular diseases and correlated them with the ability of the respective strains to colonize the epithelial cells and form biofilms on the inert substratum. The strains isolated from the lower respiratory tract exhibited the highest adherence capacity and were intensive biofilm producers. The SAP9, ALS3, ALS5, and ALS6 genes were the most frequently detected. There was a significant association between the presence of ALS 3 gene and the cellular substrate colonizing potential of the harboring strains. We also found that the strains expressing SAP9 were more virulent in the phenotypic assays. Detecting the presence of adherence genes from different clinical isolates is a cost-effective tool that would allow researchers to predict the virulence of a certain strain and estimate its potential to adhere to host cells and develop biofilms.

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