Bakare, A.A., Lateef, A., Amuda, O.S., Afolabi, R.O., 2003, The aquatic toxicity and characterization of chemical and microbiological constituents of water samples from Oba River, Odo-Oba, Nigeria. Asian Journal of Microbiology, Biotechnology and Environmental Sciences, 5 : 11-17.
Bankole, M.T., Jimoh, O.T., Mohammed, I.A., AbdulKareem, A.S., 2014, A review on nanotechnology as a tool of change in Nigeria. Scientific Research and Essays, 9 : 213-223.
Bankole, O.M., 2018, Phytochemical and antimicrobial studies of colloidal silver
Peter Kaštier, Michaela Martinčová, Roderik Fiala and Alžbeta Blehová
Dodder (Cuscuta) species cause severe agricultural damage in many countries throughout the world. To establish strategies for control of its growth and spreading it is important to study its life cycle and survival strategies. For these efforts genetic modification would represent a powerful tool. Here we report on Agrobacteriummediated transformation of dodder using green fluorescent protein (GFP) fused to actin-binding protein as a vital marker. Since the shoot of germinating C. europaea contains a functional apical meristem and grows quickly comparing to the root-like structure, the shoot apex was used here as explant. The transgene expression was only transient, nevertheless it enabled to detect allocation of actin filaments and studying the cytoskeleton organization in dodder shoot apex. Transient expression of GFP appears to be a suitable method for studying Cuscuta development through cytoskeleton organisation that is presently largely unexplored.
Pierfausto Seneci, Giorgio Fassina, Vladimir Frecer and Stanislav Miertus
MORTIER, J., RAKERS, C., FREDERICK, R., WOLBER, G.: Computational tools for in silico fragment-based drug design. Curr. Top. Med. Chem., 12, 2012, 1935-1943.
NAIK, A. D., MENEGATTI, S., GURGEL, P. V., CARBONELL, R. G.: Performance of hexamer peptide ligands for affinity purification of immunoglobulin G from commercial cell culture media. J. Chromatogr. A, 1218, 2011, 1691-700.
NIXON, A.E.: Phage Display as a Tool for Protease Ligand Discovery. Curr. Pharm. Biotechnol., 3, 2002, 1-12.
NOPPE, W., PLIEVA, F. M., GALAEV, I. Y
Vladimír Frišták, Michaela Valovčiaková, Martin Pipíška and Jozef Augustín
Production of unsettleable sewage sludge with high water content is one of the problems of intensification of industrial activities and environmental protection. Sewage sludge with low toxic metals concentrations can be utilized as fertilizer and soil conditioner in agriculture. For determination of metal bioavailability, a wide range of extraction protocols and fractionation analyses can be used. We studied the distribution and quantified the leaching and bioavailability of zinc from dried anaerobic sludge by simultaneous, multi-step and three different sequential extraction protocols. For determination of zinc, the galvanostatic stripping chronopotentiometry (SCP) and electrothermal atomic absorption spectrometry (ETAAS) were used. The distribution of Zn in sequential extraction protocols was determined using a fivestep chemical fractionation procedures (BCR, Tessier and Van Hullebusch protocols). The potential bioavailability (0.9% NaCl, 0.1 mol/dm3 HCl, 0.1 mol/dm3 HNO3, 0.1 mol/dm3 CH3COOH, 0.1 mol/dm3 Na2EDTA, 0.1 mol/dm3 CaCl2, 0.1 mol/dm3 MgCl2, 0.1 mol/dm3 (NH4)2C2O4.H2O extraction) and pseudo total (aqua regia extraction and ETAAS analyses) content of Zn in sludge was determined. The amount of aqua regia extractable zinc in sludge samples was 650 ± 12 mg/kg (d.w.). We found out that the zinc was extractable from anaerobic sludge in first hour of contact time for all tested agents. Zinc was extracted with highest efficiency by 0.1 mol/dm3 (NH4)2C2O4.H2O, 0.1 mol/dm3 HCl and 0.1 mol/dm3 Na2EDTA. Sequential extraction protocols showed that the maximum extractable amount of zinc 126.3 ± 2.6 mg Zn / kg d.w. was bound to organic matter and sulfides. High concentrations of zinc in residual fractions were leachable under extraction conditions of strong acids only.
Vladimír Frišták, Martin Pipíška, Tatiana Gablovičová and Juraj Lesný
., 406, 2008, 385-395.
LUOMA, S.N., RAINBOW, P.S.: Why is metal bioaccumulation so variable? Biodynamic as a unifying concept. Environ. Sci. Technol., 39, 2005, 1921-1931.
FRIŠTÁK, V., VALOVČIAKOVÁ, M., PIPÍŠKA, M., AUGUSTÍN, J.: Simultaneous and sequential extraction protocols as tools for determination of zinc bioavailability in dried anaerobic sludge. Nova Biotechnol. Chim., 11, 2012, 167-175.
TESSIER, A. CAMPBELL, P.G.C. BISSON, M.: Sequential extraction procedure for the speciation of particulate trace metals. Anal
Matej Planý, Tomáš Kuchta, Katarína Šoltýs, Tomáš Szemes, Domenico Pangallo and Peter Siekel
ALEGRIA, A., SZCZESNY, P., MAYO, B., BARDOWSKI, J., KOWALCZYK, M.: Biodiversity in Oscypek, a traditional Polish cheese, determined by culturedependent and -independent approaches. Appl. Environ. Microbiol., 78, 2012, 1890-1898.
ALTSCHUL, S. F., GISH, W., MILLER, W., MYERS, E. W., AND LIPMAN, D. J.: basic local alignment search tool. J. Mol. Biol., 215, 1990, 403-410.
BERTA, G., CHEBEŇOVÁ, V., BREŽNÁ, B., PANGALLO, D., VALÍK, Ľ., KUCHTA, T.: Identification of lactic acid bacteria in Slovakian bryndza
Oľga Šestinová, Lenka Findoráková and Jozef Hančuľák
This study presents the results of the testing toxicity of the contaminated sediments from the water reservoir of Ružín No.I deposit (Slovak Republic) by using Phytotoxkit tests (MicroBioTests Inc., Belgium). The Phytotoxkit system is a screening tool used for a variety of toxicity testing applications. The advantages of this toxicity bioassay are its speed, relative simplicity and low cost compared to chemical analysis and many other biotests. Evaluation of sediments phytotoxicity was based on the testing of seed germination and the assesment of the root growth decrease of the plant Sinapis alba which allows to complete the assays after only 3 days of incubacion. Chemical analysis of the sediment samples involved determination of heavy metal (Cu, Zn, Ni, As, Sb and Hg) concentration. No potential phytotoxic effect of heavy metals in contaminated sediments was observed in the majority of tested seeds of Sinapis alba.