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In vitro and in vivo evaluation of electrochemotherapy with trans-platinum analogue trans-[PtCl2(3-Hmpy)2]

.2×10 7 cells/mL were prepared via the trypsinization (Trypsin, Gibco) of two day-old monolayers. The cytotoxicity of treatments was determined using a clonogenic assay. In the EP group, 5 μL of EP buffer was added to 45 μL (1×10 6 ) of cell suspension and subsequently placed between two parallel stainless-steel plate electrodes at a 2-mm distance and thereafter exposed to 8 electric pulses (electric field over distance ratio of 1300 V/cm, duration time of each pulse 100 μs, at frequency of 1 Hz; generated at Jouan GHT 1287, St. Herblain, France). 26 , 27 In CDDP or

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