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Marcin Samiec and Maria Skrzyszowska

Abstract

The objective of the current investigation was to extensively compare the in vitro developmental capabilities between cloned pig embryos reconstructed with the cell nuclei of either cumulus oophorus cells or adult dermal fibroblast cells that were both evaluated as non-apoptotic on the basis of YO-PRO-1- and Annexin V-eGFP-mediated vital analysis for programmed cell suicide. In Group I, the competences of nuclear-transferred (NT) embryos that were derived from non-apoptotic/ non-necrotic (i.e., YO-PRO-1- and Annexin V-eGFP-negative) cumulus cells to complete their development to the morula and blastocyst stages were maintained at the proportions of 155/364 (42.6%) and 54/364 (14.8%), respectively. In Group II, NT embryos that were reconstituted with non-apoptotic and/or non-necrotic adult cutaneous fibroblast cells developed to the morula and blastocyst stages at the rates of 207/358 (57.8%) and 110/358 (30.7%), respectively. Although the in vitro developmental potential of porcine NT embryos derived from non-apoptotic/non-necrotic cumulus cells was significantly lower (P<0.001) than that of NT embryos reconstructed with adult dermal fibroblast cells, the obtained morula/blastocyst formation rates turned out to be considerably higher as compared to the rates reported by other investigators. Altogether, to our knowledge, the comprehensive research aimed at the determination of preimplantation developmental outcomes of cloned pig embryos produced using nuclear donor somatic cells of different provenance (cumulus oophorus cells or adult cutaneous fibroblast cells) that were vitally diagnosed for the lack of proapoptotic transformations in their plasma membranes has not yet been accomplished.

Open access

G. Hrčková, E. Vendelova and S. Velebný

containing β-glucan. Immunology, 104: 198 – 206 10.1046/j.1365-2567.2001.01291.x Reichner JS Fitzpatrick PA Wakshull E Albina JE 2001 Receptor-mediated phagocytosis of rat macrophages is regulated differentially for opsonized particles and non-opsonized particles containing β-glucan Immunology 104 198 206 Ruckerl, D., Allen, J.E. (2014): Macrophage proliferation, provenance and plasticity in macroparasite infection. Immunol. Rev., 262: 113 – 133. DOI: 10.1111/imr.12221 10.1111/imr.12221 Ruckerl D Allen JE 2014 Macrophage proliferation, provenance and plasticity in

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Timea Hajnal-Jafari, Simonida Đurić, Dragana Stamenov, Verica Vasić and Davorka Hackenberger

Mass fur die Mikroorganizmentatigk eit im Boden. Z. Pflansenern. Dung Bodenkude, 73: 1-11, 1956. LIPSON D.A., SCHMIDT S.K.: Seasonal Changes in an Alpine Soil Bacterial Community in the Colorado Rocky Mountains. Applied Environmental Microbiology, 70 (5): 2867-2879, 2004. MANNINEN A.M., TARHANEN S., VUORINEN M., KAINULAINEN P.: Comparing the variation of needle and wood terpenoids in Scots pine provenances. Journal of Chemical Ecology, 28: 211–227, 2002. MENYAILO O.V., HUNGATE B.A., ZECH W.: Tree species mediated soil chemical changes in a Siberian