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Jean Barloy, Stephane Dréano, Frederique Barloy-Hubler, Florin Prunar, Silvia Prunar and Aline Primot

Abstract

C. (Morphocarabus) rothi comptus Dejean 1831, is a endemic species of the Romania, appearing localized to some mountainous massifs of Romanian Banat and their piedmont areas (Poiana Rusca Mountains, Țarcu Mountains). Its presence in the Apuseni Mountains is doubtful, due to the lack of recent captures and to the confusion with C. (Morphocarabus) hampei diffinis Csiki 1905, morphologically like. In Banat the species occurs in two forms: szorenyensis Csiki 1908 at higher altitude (1300-2000 m) and ulrichhoffmanni Lie 1982 in hilly areas, which is distinguished by a larger size. Whatever the altitude and the ecological conditions, the period of activity is brief, spread over a month with a maximum of over two weeks. Molecular biology studies: confirm the belonging of comptus to rothi as a subspecies of her (28SRNA5 marker); suggest (concatenation COI I / cyt b) a low infra-specific variability between provenances of the szorenyensis (Rusca, Muntele Mic) and between the origins of the ulrichhoffmanni.

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Marcin Samiec and Maria Skrzyszowska

Abstract

The objective of the current investigation was to extensively compare the in vitro developmental capabilities between cloned pig embryos reconstructed with the cell nuclei of either cumulus oophorus cells or adult dermal fibroblast cells that were both evaluated as non-apoptotic on the basis of YO-PRO-1- and Annexin V-eGFP-mediated vital analysis for programmed cell suicide. In Group I, the competences of nuclear-transferred (NT) embryos that were derived from non-apoptotic/ non-necrotic (i.e., YO-PRO-1- and Annexin V-eGFP-negative) cumulus cells to complete their development to the morula and blastocyst stages were maintained at the proportions of 155/364 (42.6%) and 54/364 (14.8%), respectively. In Group II, NT embryos that were reconstituted with non-apoptotic and/or non-necrotic adult cutaneous fibroblast cells developed to the morula and blastocyst stages at the rates of 207/358 (57.8%) and 110/358 (30.7%), respectively. Although the in vitro developmental potential of porcine NT embryos derived from non-apoptotic/non-necrotic cumulus cells was significantly lower (P<0.001) than that of NT embryos reconstructed with adult dermal fibroblast cells, the obtained morula/blastocyst formation rates turned out to be considerably higher as compared to the rates reported by other investigators. Altogether, to our knowledge, the comprehensive research aimed at the determination of preimplantation developmental outcomes of cloned pig embryos produced using nuclear donor somatic cells of different provenance (cumulus oophorus cells or adult cutaneous fibroblast cells) that were vitally diagnosed for the lack of proapoptotic transformations in their plasma membranes has not yet been accomplished.