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M. Falis, I. Krupicer, J. Legáth, V. Pitoňák and M. Špalek

[1] Cox, C. (2000): Glyphosate factsheet. J. Pestic. Reform, 108(3) Retrieved November 24, 2011 from [2] Clough, J. M., Godfrey, C. R. A., Godwin, J. R., Joseph, R. S. I., Spinks, C. (1996): Azoxystrobin: a novel broadspectrum systemic fungicide. Pestic. Outl., 7(4): 16–20 [3] Čerňanská, D., Várady, M., Čorba, J. (2006): A survey on anthelmintic resistance in nematode parasites of sheep in the Slovak Republic. Vet

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Nadia Zikry Dimetry, Soad El-Gengaihi, Mahmoud Hafez and Magdy Hassan Abbass

REFERENCES 1. Kraus W, Cramer R, Bokel M, Sawitzki, G. New insect antifeedant from Azadirachta indica and Melia azedarach. In: Schmutterer H, Ascher KRS, Rembold H (eds.). Proc. 1 st Int Neem Conf Rottach-Egern 1981:53-62. 2. Jibrin DM, Abdullah J, Ibrahimn U. Evaluation of some plant products for the control of the cowpea weevil ( Callosobruchus maculatus ). Amer Euras J Agric Environ Sci 2013; 13(5):673-676. 3. Dimetry NZ. Prospects of botanical pesticides for the future in integrated pest management programme (IPM) with special reference

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Afaq Ahmad Malik, Javed Ahmad and M.Z. Abdin

. 886, 13 March 2001; UNCTAD/ WTO International Trade Centre. 19. Malik AA, Suryapani S, Ahmad J. Chemical vs organic cultivation of medicinal and aromatic plants: the choice is clear. Int J Med Arom Plants 2011; 1:5-13. 20. Aslam M. Asian medicine and its practice in Britain. In: Evans WC (ed.). Trease and Evans’ Pharmacognosy. 14th ed. London 1996:488-504. 21. Zuin VG, Vilegas JHY. Pesticide residues in medicinal plants and phytomedicines. Phytother Res 2000; 14:73-88. 22. Ernst E. Heavy metals in

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Ghaleb Tayoub, Malik Alorfi and Hala Ismail

)90016-3 15. Prakash A, Rao J. Botanical Pesticides in Agriculture. CRC Press, Inc., 2000 Corporate Bld., N.W., Boca Raton, FL, USA 1997; pp. 480. 16. Papachristos DP, Karamanoli KI, Stamopoulos DC, Menkissoglu-Spiroudi U. The relationship between the chemical composition of three essential oils and their insecticidal activity against Acanthoscelides obtectus (Say). Pest Manag Sci 2004; 60(5):514-520. doi: 17. Tayoub G, Abu Alnaser A, Ghanem I. Fumigant activity of leaf essential oil from Myrtus communis L. against the

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N. Thligene, G. N. Mezzapesa, D. Mondelli, A. Trani, P. Veronico, M. T. Melillo, S. Dumontet, T. Miano and N. Sasanelli


Plant parasitic nematodes (PPN) are important pests of numerous agricultural crops especially vegetables, able to cause remarkable yield losses correlated to soil nematode population densities at sowing or transplant. The concern on environmental risks, stemming from the use of chemical pesticides acting as nematicides, compels to their replacement with more sustainable pest control strategies. To verify the effect of aqueous extracts of the agro-industry waste coffee silverskin (CS) and brewers’ spent grain (BSG) on the widespread root-knot nematode Meloidogyne incognita, and on the physiology of tomato plants, a pot experiment was carried out in a glasshouse at 25 ± 2 °C. The possible phytotoxicity of CS and BSG extracts was assessed on garden cress seeds. Tomato plants (landrace of Apulia Region) were transplanted in an artificial nematode infested soil with an initial population density of 3.17 eggs and juveniles/mL soil. CS and BSG were applied at rates of 50 and 100 % (1L/pot). Untreated and Fenamiphos EC 240 (nematicide) (0.01 μL a.i./mL soil) treated plants were used as controls. Reactive oxygen species (ROS) and chlorophyll content of tomato plants were estimated during the experiment. CS extract, at both doses, significantly reduced nematode population in comparison to the untreated control, although it was less effective than Fenamiphos. BSG extract did not reduce final nematode population compared to the control. Ten days after the first treatment, CS 100 %, BSG 50 % and BSG 100% elicited the highest ROS values, which considerably affected the growth of tomato plants in comparison to the untreated plants. The control of these pests is meeting with difficulties because of the current national and international regulations in force, which are limiting the use of synthetic nematicides. Therefore, CS extracts could assume economic relevance, as alternative products to be used in sustainable strategies for nematode management.

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Mukesh Kumar Chaubey

M, Koshy M, Kow Y W, Nazar-Stewart V, Kisby GE. Biomarkers of oxidative stress and DNA damage in agricultural workers: a pilot study. Toxicol Appl Pharmacol 2008; 227(1):97-107 9. Simoniello M F, Kleinsorge EC, Scagnetti JA, Grigolato RA, Poletta GL, Carballo MA. DNA damage in workers occupationally exposed to pesticide mixtures. J Appl Toxicol 2008; 28(8):957-65 10. Le Goff J, Andre V, Lebailly P, Pottier D, Perin F, Perin O, Gauduchon P. Seasonal variations of DNA-adduct patterns in open field farmers handling pesticides. Mutat Res 2005; 587(1-2):90-102 11

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A. Čerevková and Ľ. Cagáň

.2134/agronj2010.0236 [10] FAO: Clothianidin (2010): Retrieved from online. [11] Ferris, H., Bongers, T., De Goede, R. G. M. (2001): A framework for soil food web diagnostics. extension of the nematode faunal analysis concept. Appl. Soil Ecol., 18(1): 13–29. DOI: 10.1016/S0929-1393(01)00152-4 [12] Freckman, D. W., Ettema, C. H. (1993): Assessing

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Á. Daragó, M. Szabó, K. Hrács, A. Takács and P. Nagy

[1] Affokpon, A., Coyne, D. L., Htay, C. C., Agbédé, R. D., Lawouin, L., Coosemans, J. (2011): Biocontrol potential of native Trichoderma isolates against root-knot nematodes in West African vegetable production systems. Soil Biol. Biochem., 43: 600–608. DOI: 10.1016/j.soilbio.2010.11.029 [2] Atreya, K. (2008): Health costs from short-term exposure to pesticides in Nepal. Soc. Sci. Med., 67: 511–519. DOI: 10.1016/j.socscimed.2008.04.005.

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Iyad Ghanem, Adnan Audeh, Amer Abu Alnaser and Ghaleb Tayoub

References 1. Mueller DK. Fumigation. In: Mallis, A. (ed.). Handbook of Pest Control. Franzak and Foster Co. Cleveland 1990:901-939. 2. Prakash A, Rao J. Botanical Pesticides in Agriculture. CRC Press 1997: 480. 3. Lowe S, Browne M, Boudjelas S, DePoorter M. 100 of the World’s Worst Invasive Alien Species: a selection from the global invasive species database. Invasive species specialist Group, World Conservation Union (IUCN) 2000. Available at Accessed 27 September 2005

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J. Pavlinová, J. Kinčeková, A. Ostró, L. Saksun, Z. Vasilková and A. Königová

): Pharmacological possibilities of embryoprotective therapy of infertile women. Klin. Farmakol. Farm., 23(2): 76–82 (In Czech) [15] Jirsová, S., Mašata, J., Jech, L., Drbohlav, P., Jaržembovská, M., Pavelková, J., Moosová, M., Řežábek, K., Bencko, V., Zvárová, J. (2009): Levels of chlorinated organic pesticides in follicular liquid of infertile women. Chem. Listy. 103: 589–594 (In Czech) [16] Krajčovičová, R., Hudeček, R., Kalvodová, J. (2007): Differential diagnostics and therapy of repeated pregnancy losses. Prakt. Gyn. 11