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Pavel Janoš, Jakub Ederer and Marek Došek

ability versus degradation efficiency. Adv. Mater. Sci. Eng., 2014b, Article ID 706041. JANOŠ, P., KURÁŇ, P., PILAŘOVÁ, V., TRÖGL, J., ŠŤASTNÝ, M., PELANT, O., HENYCH, J., BAKARDJIEVA, S., ŽIVOTSKÝ, O., KORMUNDA, M., MAZANEC, K., SKOUMAL, M.: Magnetically separable reactive sorbent based on the CeO2/γ-Fe2O3 composite and its utilization for rapid degradation of the organophosphate pesticide parathion methyl and certain nerve agents. Chem. Eng. J., 262, 2015, 747-755. JANOŠ, P., NOVÁK, J., BROUL, M.: Preparation of ceria-based polishing

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Silvia Zichová, Adriana Brisudová and Svetlana Hrouzková

References Agrawal K, Wu FH (2007) Drop-to-drop solvent microextraction coupled with gas chromatography/mass spectrometry for rapid determination of trimeprazine in urine and blood of rats: application to pharmacokinetic studies. Rapid Commun. Mass Spectrom. 21: 3352-3356. Ahmadi F, Assadi Y, Hosseini SM, Rezaee M (2006) Determination of organophosphorus pesticides in water samples by single drop microextraction and gas chromatography-flame photometric detector. J. Chromatogr. A 1101: 307-312. Ahmadi-Jouibari T, Fattahi N, Shamsipur M (2014

Open access

Jose Isagani B. Janairo, Patricia Isabel K. Bravo, Ninna Louise G. Morano and Derrick Ethelbhert C. Yu


The Anopheles gambiae is a highly anthropophilic mosquito which is the leading vector for malaria. This disease has affected more than 500 million people worldwide. The Anopheles gambiae targets its hosts through the odors of the human skin and sweat where odorant molecules radiate. These odors elicit specific responses from the insect through the odorant – binding proteins (OBP). Recently, a specific type of OBP has been characterized which is known as the Anopheles gambiae odorant – binding protein 20 (AgamOBP20). This OBP is highly expressed in the female mosquito antennae during the peak of its host – seeking behavior and thus may play a role in olfactory perception. The binding site of the AgamOBP20 is composed primarily of hydrophobic residues wherein the importance of each residue is herein analysed to further understand the properties of AgamOBP20. This was carried out through computer – aided site – directed mutagenesis coupled with homology modelling and docking simulations wherein each residue in the binding site was changed to alanine and serine. Probable key amino acid residues were identified as LEU106, LEU107, and MET53 which are hypothesized to play a significant role in the protein – ligand interaction. These residues had the greatest impact in the binding free energy when mutated with alanine and serine. The presented results suggest that steric hindrance and hydrophobic interaction are crucial factors to consider on the manner in which the ligand binds with AgamOBP20. The molecular features and parameters obtained may be utilized for the development of new pesticides and repellents that are able to block the function of AgamOBP20 and may result to the disarray of the host – seeking behavior of the Anopheles gambiae.

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Andrea Nagy, Gábor Simon and István Vass

. Pollut. Bull., 31, 1995, 237-48. YUNKER, M.B., MACDONALD, R.W., VINGARZAN, R., MITCHELL, H.R. GOYETTE, D., SYLVESTRE, S.: PAHs in the Fraser River basin: a critical appraisal of PAH ratios as indicators of PAH source and composition. Organic Geochem., 33, 2002, 489-515. ZHANG, Z., HUANG, J., YU, G., HONG, H.: Occurrence of PAHs, PCBs and organochlorine pesticides in the Tonghui River of Beijing, China. Environ. Pollut. 130, 2004, 249-261.

Open access

Simona Kvasnová, Ľudmila Hamarová and Peter Pristaš

References Ahmed MJ, Alam M (2003) A rapid spectrophotometric method for the determination of mercury in environmental, biological, soil and plant samples using diphenylthiocarbazone. Spectroscopy 17: 45-52. Alvarez A, Saez JM, Costa JSD, Colin VL, Fuentes, MS, Cuozzo SA, Amoroso MJ (2017) Actinobacteria: Current research and perspectives for bioremediation of pesticides and heavy metals. Chemosphere 166: 41-62. Boriová K, Urík M, Matus P (2015) Biosorption, bioaccumulation, biovolatilization of potentially

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Pranjal Bharali, Salam Pradeep Singh, Yasir Bashir, Nipu Dutta, Bolin Kumar Konwar and Chingakham Brajakishor Singh

agriculture and biomedicine. Appl. Microbiol. Biotechnol. 101: 8309-8319. De S, Malik S, Ghosh A, Saha R, Saha B (2015) A review on natural surfactants. RSC Adv. 5: 65757-65767. García-Reyes S, Yáñez-Ocampo G, Wong-Villarreal A, Rajaretinam RK, Thavasimuthu C, Patiño R, Ortiz- Hernández ML (2017) Partial characterization of a biosurfactant extracted from Pseudomonas sp. B0406 that enhances the solubility of pesticides. Environ. Technol. 22: 1-10. Haba E, Pinazo A, Jauregui O, Espuny MJ, Infante MR, Manresa A (2003

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Przemysław Kosobucki and Bogusław Buszewski

References ABRAMOV, E.G., BEZZUBOV, A.A.: Electrosorptive separation of humic substances. J. Water Chem. Technol., 29, 2007, 125-130. ANDRÉ, C., GUILLAUME, Y.C.: CEC for Studying the Retention and Separation of Pesticides on a Humic Acid Stationary Phase. Chromatographia, 68, 2008, 791-796. BAXTER, R.M., MALYSZ, J.: Analysis of aquatic humic material and high molecular weight components of bleached kraft mill effluent (BKME) by gradient gel electrophoresis. Chemosphere, 12, 1992, 1745