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Analysis of expression of genes responsible for regulation of cellular proliferation and migration – microarray approach based on porcine oocyte model

fertilization process stops the second arrest and terminates meiosis. The duration of oocyte full maturation is dependent on the species, in humans it can last decades [ 4 ]. In in vitro conditions, nuclear maturation is hormone-independent and is initiated by removing the oocytes from their follicular environment [ 5 ]. The proper oocyte development requires close communication with surrounding somatic cells. These cells are affected by wide range of regulatory molecules, proteins and hormones leading to proper oocyte maturation. The somatic cumulus cells (CCs) and

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The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

, according to the technical protocol, using the Affymetrix GeneAtlas Fluidics Station. The array strips were scanned employing the Imaging Station of the GeneAtlas System. Preliminary analysis of the scanned chips was performed using Affymetrix GeneAtlasTM Operating Software. The quality of gene expression data was confirmed according to the quality control criteria provided by the software. The obtained CEL files were imported into downstream data analysis software. All of the presented analyses and graphs were compiled using Bioconductor and R programming languages

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Genes encoding proteins regulating fatty acid metabolism and cellular response to lipids are differentially expressed in porcine luminal epithelium during long-term culture

chips was performed using Affymetrix GeneAtlasTM Operating Software. The quality of gene expression data was confirmed according to the quality control criteria provided by the software. The obtained CEL files were imported into downstream data analysis software. All of the presented analyses and graphs were compiled using Bioconductor and R programming languages. Each CEL file was merged with a description file. To correct background, normalize, and summarize results, we used the Robust Multiarray Averaging (RMA) algorithm. To determine the statistical significance

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Analysis of the Variability of Epstein-Barr Virus Genes in Infectious Mononucleosis: Investigation of the Potential Correlation with Biochemical Parameters of Hepatic Involvement

Res 2009; 143: 209-21. 13. Bhatia K, Raj A, Guitierrez MI, Judde JG, Spangler G, Venkatesh H, et al. Variation in the sequence of Epstein- Barr virus nuclear antigen 1 in normal peripheal blood lymphocytes and in Burkitt’s lymphomas. Oncogene 1996; 13: 177-81. 14. Sandvej K, Zhou XG, Hamilton-Dutoit S. EBNA-1 sequence variation in Danish and Chinese EBV-associated tumors: evidence for geographical polymorphism but not for tumor-specific subtype restriction. J Pathol 2000; 191: 127-31. 15. Lorenzetti MA, Altcheh J

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The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens Variables

References Babizhayev MA. Failure to withstand oxidative stress induced by phospholipid hydroperoxides as a possible cause of the lens opacities in systemic diseases and ageing. Biochim Biophys Acta 1996; 1315: 87-99. Garner B, Davies MJ, Truscott RJW. Formation of hydroxyl radicals in the human lens is related to the severity of nuclear cataract. Exp Eye Res 2000; 70: 81-8. Lou MF. Redox regulation in the lens. Prog Ret Eye Res 2003; 22: 657-82. Kisić B

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Association Between Oxidative Stress and Melanoma Progression

, Epidemiology, and End Results (SEER) Program. Oncologist 2007; 12(1): 20-37. 4. Ali Z, Yousaf N, Larkin J. Melanoma epidemiology, biology and prognosis. EJC Suppl 2013; 11(2): 81-91. 5. Zhang Z, Zhu S, Yang Y, Ma X, Guo S. Matrix metalloproteinase- 12 expression is increased in cutaneous melanoma and associated with tumor aggressiveness. Tumour Biol 2015; 36(11): 8593-600. 6. Swetter SM, Clarke CA, Keegan THM. Why Do Men Have Worse Melanoma Survival than Women? Is It Behavior, Biology, or Both? The Melanoma Letter Summer 2014

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Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

References 1. Jamnongjit M, Hammes SR. Oocyte maturation: the coming of age of a germ cell. Semin Reprod Med. 2005;23:234–41; DOI:10.1055/s-2005-872451. 2. Rybska M, Knap S, Jankowski M, Jeseta M, Bukowska D. Cytoplasmic and nuclear maturation of oocytes in mammals – living in the shadow of cells developmental capability. Med J Cell Biol. 2018;1; DOI:10.2478/acb-2018-0003. 3. Coticchio G, Dal Canto M, Renzini MM, Guglielmo MC, Brambillasca F, Turchi D, Novara PV, Fadini R. Oocyte maturation: Gamete-somatic cells interactions, meiotic resumption

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Application of targeted next generation sequencing for the mutational profiling of patients with acute lymphoblastic leukemia

leukemia treated by the NOPHO ALL-2008 protocol. Eur J Haematol 2013; 90: 404–12. 10.1111/ejh.12097 Toft N Birgens H Abrahamsson J Bernell P Griškevičius L Hallböök H et al Risk group assignment differs for children and adults 1-45 yr with acute lymphoblastic leukemia treated by the NOPHO ALL-2008 protocol Eur J Haematol 2013 90 404 – 12 6 Mullighan CG. The molecular genetic makeup of acute lymphoblastic leukemia. Hematology Am Soc Hematol Educ Program 2012: 389–96. Mullighan CG The molecular genetic makeup of acute lymphoblastic

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Diagnostic and Therapeutic Significance of the Oxidative Stress Parameters in Children

; 34: 1217-20. Monici MC, Aguennouz M, Mazzeo A, Messina C, Vita G. Activation of nuclear factor-kappaB in inflammatory myopathies and Duchenne muscular dystrophy. Neurology 2003; 60: 993-7. Gupta M, Gupta YK, Agarwal S, Aneja S, Kalaivani M, Kohli K. Effects of add-on melatonin administration on antioxidant enzymes in children with epilepsy taking carbamazepine monotherapy: a randomized, double-blind, placebo-controlled trial. Epilepsia 2004; 45: 1636-9. Hamed SA, Abdellah MM, El-Melegy N

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The Role of Pharmacogenetics in the Treatment of Diabetes Mellitus / ULOGA FARMAKOGENETIKE U LEČNJU DIJABETES MELITUSA

tolerance or impaired fasting glucose: a randomised controlled trial. Lancet 2006; 368: 1096-105. [PubMed] 3. Knowler WC, Barrett-Connor E, Fowler SE, Hamman RF, Lachin JM, Walker EA, Nathan DM. Reduction in the incidence of type 2 diabetes with lifestyle intervention or metformin. N Engl J Med 2002; 346: 393-403. (PMC free article) [PubMed] 4. Knowler WC, Hamman RF, Edelstein SL, Barrett-Connor E, Ehrmann DA, Walker EA, Fowler SE, Nathan DM, Kahn SE. Prevention of type 2 diabetes with troglitazone in the Diabetes Prevention Program

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