Search Results

1 - 10 of 71 items :

  • nuclear program x
  • Molecular Biology x
Clear All
Analysis of expression of genes responsible for regulation of cellular proliferation and migration – microarray approach based on porcine oocyte model

fertilization process stops the second arrest and terminates meiosis. The duration of oocyte full maturation is dependent on the species, in humans it can last decades [ 4 ]. In in vitro conditions, nuclear maturation is hormone-independent and is initiated by removing the oocytes from their follicular environment [ 5 ]. The proper oocyte development requires close communication with surrounding somatic cells. These cells are affected by wide range of regulatory molecules, proteins and hormones leading to proper oocyte maturation. The somatic cumulus cells (CCs) and

Open access
The genes regulating maintenance of cellular protein location are differentially expressed in porcine epithelial oviductal cells during longterm in vitro cultivation

, according to the technical protocol, using the Affymetrix GeneAtlas Fluidics Station. The array strips were scanned employing the Imaging Station of the GeneAtlas System. Preliminary analysis of the scanned chips was performed using Affymetrix GeneAtlasTM Operating Software. The quality of gene expression data was confirmed according to the quality control criteria provided by the software. The obtained CEL files were imported into downstream data analysis software. All of the presented analyses and graphs were compiled using Bioconductor and R programming languages

Open access
Genes encoding proteins regulating fatty acid metabolism and cellular response to lipids are differentially expressed in porcine luminal epithelium during long-term culture

chips was performed using Affymetrix GeneAtlasTM Operating Software. The quality of gene expression data was confirmed according to the quality control criteria provided by the software. The obtained CEL files were imported into downstream data analysis software. All of the presented analyses and graphs were compiled using Bioconductor and R programming languages. Each CEL file was merged with a description file. To correct background, normalize, and summarize results, we used the Robust Multiarray Averaging (RMA) algorithm. To determine the statistical significance

Open access
ARTs in wild felid conservation programmes in Poland and in the world

no doubt that implementation of advanced wild cat conservation programmes is essential. Wild felid conservation involves application of in situ methods, when a given species occurs in the natural habitat, or ex situ methods in centres with a conservation mandate ( e.g . reserves or zoos) by taking advantage of the latest advances in biotechnology, cryobiology, and bioinformatics. Besides raising and breeding animals in captivity, ex situ methods include preservation of their genetic material, usually in cell banks. Projects aimed at conservation of the

Open access
Analysis of the Variability of Epstein-Barr Virus Genes in Infectious Mononucleosis: Investigation of the Potential Correlation with Biochemical Parameters of Hepatic Involvement

Res 2009; 143: 209-21. 13. Bhatia K, Raj A, Guitierrez MI, Judde JG, Spangler G, Venkatesh H, et al. Variation in the sequence of Epstein- Barr virus nuclear antigen 1 in normal peripheal blood lymphocytes and in Burkitt’s lymphomas. Oncogene 1996; 13: 177-81. 14. Sandvej K, Zhou XG, Hamilton-Dutoit S. EBNA-1 sequence variation in Danish and Chinese EBV-associated tumors: evidence for geographical polymorphism but not for tumor-specific subtype restriction. J Pathol 2000; 191: 127-31. 15. Lorenzetti MA, Altcheh J

Open access
The Impact of Senile Cataract Maturity on Blood Oxidative Stress Markers and Glutathione-Dependent Antioxidants: Relations with Lens Variables

References Babizhayev MA. Failure to withstand oxidative stress induced by phospholipid hydroperoxides as a possible cause of the lens opacities in systemic diseases and ageing. Biochim Biophys Acta 1996; 1315: 87-99. Garner B, Davies MJ, Truscott RJW. Formation of hydroxyl radicals in the human lens is related to the severity of nuclear cataract. Exp Eye Res 2000; 70: 81-8. Lou MF. Redox regulation in the lens. Prog Ret Eye Res 2003; 22: 657-82. Kisić B

Open access
Association Between Oxidative Stress and Melanoma Progression

, Epidemiology, and End Results (SEER) Program. Oncologist 2007; 12(1): 20-37. 4. Ali Z, Yousaf N, Larkin J. Melanoma epidemiology, biology and prognosis. EJC Suppl 2013; 11(2): 81-91. 5. Zhang Z, Zhu S, Yang Y, Ma X, Guo S. Matrix metalloproteinase- 12 expression is increased in cutaneous melanoma and associated with tumor aggressiveness. Tumour Biol 2015; 36(11): 8593-600. 6. Swetter SM, Clarke CA, Keegan THM. Why Do Men Have Worse Melanoma Survival than Women? Is It Behavior, Biology, or Both? The Melanoma Letter Summer 2014

Open access
Genetic evaluation of Cryptomeria japonica breeding materials for male-sterile trees

, Ujino-Ihara T, Uchiyama K, Futamura N, Saito M, Ueno S, Matsumoto A, Tani N, Taira H, Sninohara K, Tsumura Y (2012) The construction of a high-density linkage map for identifying SNP markers that are tightly linked to a nuclear-recessive major gene for male sterility in Cryptomeria japonica D. Don. BMC Genomics 13:95. https://doi.org/10.1186/1471-2164-13-95 Queller DC, Goodnight KF (1989) Estimating relatedness using molecular markers. International Journal of Organic Evolution 43:258–275. https://doi.org/10.1111/j.1558-5646.1989.tb04226.x Saito M

Open access
Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

References 1. Jamnongjit M, Hammes SR. Oocyte maturation: the coming of age of a germ cell. Semin Reprod Med. 2005;23:234–41; DOI:10.1055/s-2005-872451. 2. Rybska M, Knap S, Jankowski M, Jeseta M, Bukowska D. Cytoplasmic and nuclear maturation of oocytes in mammals – living in the shadow of cells developmental capability. Med J Cell Biol. 2018;1; DOI:10.2478/acb-2018-0003. 3. Coticchio G, Dal Canto M, Renzini MM, Guglielmo MC, Brambillasca F, Turchi D, Novara PV, Fadini R. Oocyte maturation: Gamete-somatic cells interactions, meiotic resumption

Open access
Plasma metabolic characterisation of dairy cows with inactive ovaries and oestrus during the peak of lactation

activins which had a positive effect on follicular growth. Moreover, Salesh et al . ( 21 ) reported that insulin-like growth factor-1 (IGF-1), thyroxine, and energy-related metabolites were positively correlated with IO and closely related to reproduction. However, these studies focused on the effect of a specific endocrine or energy pathway-related factor on the normal ovarian cycle and there is only a report on metabolic profiles of postpartum dairy cows with ovarian inactivity using 1 H-nuclear magnetic resonance spectroscopy. Prior to this article, the full extent

Open access