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Mirele S. Mialich, Bruna R. Silva and Alceu A. Jordao

:// 10.1079/BJN2001382 Dudeja V Misra A Pandey RM Devina G Kumar G Vikram NK. BMI does not accurately predict overweight in Asian Indians in northern India Br J Nutr 2001 86 105 112 23 Oh SW, Shin S, Yun YH, Yoo T, Huh B. Cut-off point of BMI and obesity-related comorbidities and mortality in middle-aged Koreans. Obesity Research 2004; 12(12): 2031-40. 10.1038/oby.2004.254 Oh SW Shin S Yun YH Yoo T Huh B. Cut-off point of BMI, obesity-related comorbidities and mortality in

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Oliver Pabst

consequently be seen as non-linear electrical measurements (on human skin) [ 22 ]. Furthermore, these can be seen as part of Bioimpedance, since this field encompasses the passive electrical properties (memristor = fourth passive electrical circuit element) of organic tissues and corresponding recording techniques [ 23 ]. Materials and methods Subject recruitment, approval The measurements were conducted on 28 test subjects (16 male, 12 female, mean age 31 years, SD = 9.5 years; all gave informed consent for participation in the study) at the University of Oslo in

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M. Amini, J. Hisdal and H. Kalvøy

cell cultivation process is performed over time ( 53 ). For impedance measurements in 3D culture systems, cells are not seeded as a monolayer on the surface of the electrode but are encapsulated in a 3D scaffold which is in contact with a conductive medium ( 52 ). Thus, the electrodes can be placed for example outside the extracellular matrix and in the middle to provide impedance measurements. Therefore, the ionic variations of the medium over time would lead to changes in conductivity which would affect the measured impedance ( 43 ). Microfluidic 3D cell