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Elena Pavlovna Kiseleva, Konstantin Igorevich Mikhailopulo, Alena Ivanovna Ladutska and Galina Ivanovna Novik

Abstract

Background: Nonpathogenic Bacillus strains are used in biotechnology, and pathogenic Bacillus strains are cause of food borne disease. It explains the relevance of the methods of detection and quantification of whole cell and cell components of these bacteria. Aims: Development of methodological approach for investigation of dynamics of specific concentration of cell wall antigens per cell of bacilli without solubilization of cell wall during sample preparation; using of the approach with 6 strains of bacilli as an example. Method: ELISA. Results: Methodological approach for investigation of dynamics of specific concentration of bacilli cell wall antigens has been developed. The distinctive features of the approach are rabbit polyclonal antibodies to genera-specific antigens of bacilli as key reagent and lack of need for solubilization of cell wall during sample preparation. It was shown using 6 strains of Bacilli as an example that specific concentration of cell wall antigens per cell vary according to bacillus strain, stage of culture growth and media composition. The data will find an application in biotechnology of clinical diagnostics and test-systems for food control including detection of whole bacillus cells.

Open access

Mark Nuijten

References 1. Nuijten MJ, Dubois DJ. Cost-utility analysis: current methodological issues and future perspectives. Front Pharmacol. 2011 Jun 8;2:29. doi: 10.3389/fphar.2011.00029. eCollection 2011. 2. Weinstein MC, Fineberg HV. Clinical decision analysis. Philadelphia (PA): WB Saunders Co., 1980: 228-65. 3. Thomas L Saaty. The Analytical Hierarchy Process (New York: McGrwa-Hill 1980). 4. Jariath N, Weinstein J. The Delphi methodology (part two): a useful administrative approach. CJONA 1994; 7

Open access

Şapcaliu Agripina, Vasilică Savu, Ion Radoi, Dana Tapaloagă, Petruţ Tanase and Victor Calin

Abstract

In order to obtain a natural apiphytotherapeutic formula with anti-parasite effect, as an alternative in the control of Nosema spp. infection, an original formula based on hydro-alcoholic extracts from plants and propolis was tested. Experiments were made on naturally infected adult bees from private apiaries diagnosed with nosemosis (Nosema apis/Nosema ceranae). Initially hydro-alcoholic extracts from plants in Romania’s flora and respectively from propolis (Apis mellifera), with sensorial, physical- chemical and microbiologic characteristics, in conformity with requirements in the European Pharmacopeia VIth Edition and Romanian Pharmacopeia Xth edition. Parasitosis was demonstrated through laboratory methods, respectively through direct microscopic examination, on bee samples collected from aviaries according to O.I.E. (World Organisation for Animal Health) methodology (2008). „In vitro“ lab testing of plant extracts and of work variants was done firstly on naturally infested polyfloral honey having an average concentration of 7 spores/ microscopic field of Nosema sp., with the purpose of selecting the most effective work variant which was afterwards tested „in vivo“ in private apiaries, on bee colonies. The results of the clinical study showed the absence of disease in 75% of the treated hives (33 hives), bees being declared clinically healthy. In 14 % of the hives (6 hives) infestation was weak, in 6.82% of the hives (3 hives) infestation was medium, and 4.55 % (2 hives) were diagnosed with a massive degree of infestation and the therapeutic protocol continued.

Open access

Mark J.C. Nuijten, Marja H. Pronk, John Hutton, Peter van Hasselt, Cor Oosterwijk and Frans F.H. Rutten

methodological issues and future perspectives. Front Pharmacol 2011. Jun 8;2:29. doi: 10.3389/fphar.2011.00029. eCollection 2011. 10. http://www.emea.europa.eu/ema/index.jsp?curl=pages/regulation/general/general_content_000029.jsp&mid=WC0b01a 11. European Medicines Agency. EPAR Myozyme. http://www.ema.europa.eu/docs/en_GB/document_library/EPAR_Scientific_Discussion/human/000636/WC500032128.pdf 12. Acquadro C, Berzon R, Dubois D. Incorporating the Patient’s Perspective into Drug Development and Communication: An Ad Hoc Task Force

Open access

Vincenza Precone, Munis Dundar, Tommaso Beccari, Eda Tahir Turanli, Stefano Cecchin, Giuseppe Marceddu, Elena Manara and Matteo Bertelli

://www.euro.who.int/en/health-topics/ health-policy/health-2020-the-european-policy-forhealth-and-well-being,accessed 6 August 2015). 42. Brown CS, Zwetyenga J, Berdieva M, Volkova T, Cojocaru R, et al. New policy-formulation methodology paves the way for sus tainable laboratory systems in Europe. Public Health Panorama. 2015;1(1):41-47. 43. Nkengasong JN, Nsubuga P, Nwanyanwu O, GershyDamet GM, RoscignoG, et al. Laboratory systems and services are critical in global health: time to end the neglect? Am J Clin Pathol. 2010;134(3):368-73. 44. Olmsted SS, Moore

Open access

Evgeni Eltzov, Abri Lavena De Cesarea, ‘Yuen Kei Adarina Low and Robert S. Marks

. Atmospheric Environment, 1997; 31(21): p. 3487-3495. 52. Wang DKW,Austin CC, Determination of complex mixtures of volatile organic compounds in ambient air: canister methodology. Analytical and Bioanalytical Chemistry, 2006; 386(4): p. 1099-1120. 53. Mui KW, Wong LT,Ho WL, Evaluation on sampling point densities for assessing indoor air quality. Building and Environment, 2006; 41(11): p. 1515-1521. 54. Praveen K. S, Eric L. B, Rangachary M,Fernando H. G, Chemical Sensors for Environmental Monitoring and Homeland Security. The Electrochemical Society