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Miroslav Ondrejovič, Daniela Chmelová and Tibor Maliar

, liriodendrin and isofraxidin in Acanthopanax senticosus Harms by HPLC-DAD. Biol. Pharm. Bull. , 29, 2006, 532-534. MYERS, R.H., MONTGOMERY, D.C.: Response surface methodology: Process and product optimization using designed experiments. Wiley, New York, 1995, 704 pp. NAVAL, M.V., GOMÉZ-SERRANILLOS, M.E., CARRETERO, M.E., VILLAR, A.M.: Neuroprotective effect of a ginseng ( Panax ginseng ) root extract on astrocytes primary culture. J. Ethnopharmacol. , 112, 2007, 262-270. PARK, H.R., PARK, E., RIM, A.R., JEON, K.I., HWANG, J

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Miroslava Hazuchová, Daniela Chmelová and Miroslav Ondrejovič

R, Gaio TA, Malvessi E, Moura da Silveira M, Dillon AJP (2011) Growth kinetics, production, and characterization of extracellular laccases from Pleurotus sajor-caju PS-2001. Process Biochem. 46: 758-764. Bhattacharya SS, Garlapati VK, Banerjee R (2011) Optimization of laccase production using response surface methodology coupled with differential evolution. New Biotechnol. 28: 31-39. Bidlack JE, Buxton DR (1992) Content and deposition rates of cellulose, hemicelluloses and lignin during regrowth of forage grasses and legumes. Can. J. Plant Sci. 72

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Barbora Micháleková Richveisová, Zuzana Dürešová, Miroslav Horník, Jozef Augustín and Martin Pipíška

Abstract

Heavy metals are taken up by the vascular plant root system from water solutions in cationic forms. Subsequently, during both short and long distance transport to other plant tissues, cation forms are incorporated to many bioorganic compounds differing in stability, ionic character and physico-chemical properties such as solubility in lipid structures and mobility across cell membrane systems. Many sequential and single step extraction methods have been elaborated for characterization of the role of individual components of plant cells components in transport and detoxication of heavy metals. In our study, dry biomass of giant reed (Arundo donax L.) grown in hydroponic media spiked with 65ZnCl2 and 109CdCl2 was treated with dithizone solutions as complexing ligand in order to convert free Zn2+ and Cd2+ ions to corresponding dithizonates. Treatment with dithizone showed that up to 67 % of the total plant Cd and 56 % of the total plant Zn were transformed to dithizonate complexes extracted with chloroform. Extraction of biomass with Folch reagent showed that up to 48 % of the total root cadmium and up to 18 % of the total shoot cadmium is bound in lipid fraction. Zinc was not found in lipid fraction of root and shoot. Derivatization of the dried root and shoot lipid fraction by dithizone showed that two third of Cd in root and practically all Cd in shoot lipid fraction could be transformed to Cd-dithizonate. Methods of biomass treating with complexing ligands and a method of sequential extraction procedures with non-polar organic solvents and radiotracer methodology seem to be useful methods for the study of metal speciation and distribution in vascular plants

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Peter Nemeček, Dáša Kružlicová and Lucia Remenárová

alkalimodified spent tea leaves using response surface methodology (RSM) and artificial neural network ANN. Appl. Water Sci., 2014, DOI 10.1007/s13201-014-0180-z. HO, Y.S., MC KAY, G.: Competitive sorption of copper and nickel ions from aqueous solution using peat. Adsorption, 5, 1999, 409-417. HO, Y.S., PORTER, J.F., MCKAY, G.: Equilibrium isotherm studies for the sorption of divalent metal ions onto peat: Copper, nickel and lead single component systems. Water, Air, and Soil Pollution, 141, 2002, 1-33. CHU, K.H. and KIM, E

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Ioanna Karageorgou, Spyros Grigorakis, Stavros Lalas and Dimitris P. Makris

determination of flavonoids with response surface methodology optimization. J. Chrom. A 1285: 22-30. Çelik SE, Özyürek M, Güçlü K, Apak R (2015) Antioxidant capacity of quercetin and its glycosides in the presence of β -cyclodextrins: influence of glycosylation on inclusion complexation. J. Inclus. Phenom. Macrocycl. Chem. 83: 309-319. Çelik SE, Özyürek M, Tufan AN, Güçlü K, Apak R (2011) Spectroscopic study and antioxidant properties of the inclusion complexes of rosmarinic acid with natural and derivative cyclodextrins. Spectrochim. Acta Part A: Mol. Biomol

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Zahir Henache, Abdelhamid Boukerroui and Imad Kashi

References AOCS (1998) Official methods sand recommended practices of American Oil Chemists’ Society. Vol. 1, 5 th ed. Champaign, IL: AOCS Press. Ajemba RO, Igbokwe PK, Onukwuli OD (2013) Optimisation of color pigments removal from palm oil by activated ukpor clay using response surface methodology. Res. J. App. Sci. Eng. Tech. 6: 423-432. Aung LL, Tertre E, Suksabye P,·Worasith N, Thiravetyan P (2015) Effect of alumina content and surface area of acid-activated kaolin on bleaching of rice bran oil. J. Am. Oil Chem. Soc. 92: 295

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Silvia Zichová, Adriana Brisudová and Svetlana Hrouzková

Souza Pinheiro A, De Andrade JB (2009) Development, validation and application of a SDME/GC-FID methodology for the multiresidue determination of organophosphate and pyrethroid pesticides in water. Talanta 79: 1354-1359. De Souza Pinheiro A, Da Rocha GO, De Andrade JB (2011) A SDME/GC–MS methodology for determination of organophosphate and pyrethroid pesticides in water. Microchem. J. 99: 303-308. Ebrahimzadeh H, Dehghani Z, Asgharinezhad AA, Shekari N, Molaei K (2013) Determination of haloperidol in biological samples with the aid of ultrasound

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Jana Kaduková, Oksana Velgosová, Anna Mražíková, Renáta Marcinčáková and Eva Tkáčová

aquatic organisms and growth inhibition tests of algae and higher cultivated plants. VIMALA, K., SIVUDU, K.S., MOHAN, Y.M., SREEDHAR, B., RAJU, K.M.: Controlled silver nanoparticles synthesis in semi-hydrogel networks of poly(acrylamide) and carbohydrates: A rational methodology for antibacterial application. Carbohyd. Polym., 75, 2009, 463-471. WANG, X.Z., YANG, Y., LI, R., MCGUINNES, C., ADAMSON, J., MEGSON I.L., DONALDSON, K.: Principal component and causal analysis of structural and acute in vitro toxicity data for nanoparticles. Nanotoxicology, 8, 5

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Daniela Chmelová and Miroslav Ondrejovič

. Wood Sci., 50, 2004, 281-284. LEVIN, L., HERRMANN, C., PAPINUTTI, V. L.: Optimization of lignocellulolytic enzyme production by the white-rot fungus Trametes trogii in solid-state fermentation using response surface methodology. Biochem. Eng. J., 39, 2008, 207-214. LEVIN, L., MELIGNANI, E., RAMOS, A. M.: Effect of nitrogen sources and vitamins on lignolytic enzyme production by some white-rot fungi. Dye decolorization by selected culture filtrates. Bioresour. Technol., 101, 2010, 4554-4563. MILLER, G. L.: Use of

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Gamal H. Ragab and Eman A. Bahgat

-2114. Hall MJ, Levant S, Defrances CJ (2012) Hospitalization for stroke in US hospitals, 1989-2009. NCHS Data Brief 95: 1-8. International Conference On Harmonization. Validation of Analytical Procedures: Text and Methodology, 2005. Jain N, Raghuwanshi R, Jain D (2008) Development and Validation of RP-HPLC method for simultaneous estimation of atorvastatin calcium and fenofibrate in tablet dosage forms. Indian J. Pharm. Sci. 70: 263-265. Khaleel N, Abdul Rahaman SK (2016) Validated stability indicating RP-HPLC method for simultaneous