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Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

H., Long B., Wu X., Bai Y., Development of a Loop-Mediated Isothermal Amplification Assay for sensitive and rapid detection of Cronobacter sakazakii. Foodborne Pathog. Dis., 2012, 9, 1111-1118. 11. Francois P., Tangomo M., Hibbs J., Bonetti E.J., Boehme C.C., Notomi T., Perkins M.D., Schrenzel J., Robustness of a loopmediated isothermal amplification reaction for diagnostic applications. FEMS Immunol. Med. Microbiol., 2011, 62, 41-48. 12. Fratamico P.M., Bagi L.K., Cray W.C. Jr., Narang N., Yan X., Medina M., Liu Y., Detection by

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Does SIRT-1 Mediate Calorie Restriction and Prolong Life? – A Mini Review

Abstract

Calorie restriction is the only intervention proved to prolong both average and maximum lifespan in yeast, worms, fish, rodents and possibly primates. Not only does the regimen prolong life, but it also reduces the incident of numerous age-related diseases like diabetes, atherosclerosis or cancer and slows down ageing. Mechanisms by which that is thought to occur have not yet been elucidated, but they probably involve reactive oxygen species signaling, insulin growth factor and transcriptional factors. Here, special emphasis is given to SIRT1 - silent information regulator. There is sound evidence showing that SIRT1 is a key player in mediating physiological response to calorie restriction and that its overexpression is correlated with extended lifespan. The possible mechanism leading to its elevated levels is high NAD/NADH ratio, observed in Sir2 in yeast. SIRT1 increases glucose production, enhances fat mobilization, stimulates angiogenesis, prevents neuronal degeneration and rises insulin sensitivity. Therefore, it seems to be a very beneficial factor activated by such a simple intervention that is calorie restriction.

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Garlic Sulfur Compounds Suppress Cancerogenesis and Oxidative Stress: a Review

-3278(96)90061-7. Benavides GA, Squadrito GL, Mills RW, Patel HD, Isbell TS, Patel RP, Darley-Usmar VM, Doeller JE, Kraus DW (2007): Hydrogen sulfide mediates the vasoactivity of garlic. Proceedings of the National Academy of Sciences of the United States of America, 104, 17977-17982. doi: 10.1073/ pnas.0705710104. Block E, Ahmad S, Jain MK, Crecely RW, Apitzcastro R, Cruz MR (1984): (E,Z)-ajoene - a potent antithrombotic agent from garlic. Journal of the American Chemical Society, 106, 8295-8296. doi: 10.1021/ja00338a049. Borek C (2001): Antioxidant health

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Repellence and attraction of Apis mellifera foragers by nectar alkaloids

Abstract

Plant secondary metabolites present naturally in nectar, such as alkaloids, may change the behavioural responses of floral visitors and affect pollination. Some studies have shown that nectar containing low concentrations of these secondary metabolites is preferred by honey bee foragers over pure nectar. However, it remains unclear whether this is caused by dependence or addictive behaviour, a simple taste preference, or by other conditions such as self-medication. In our choice experiment, free-flying bees were presented with artificial flowers holding 20% sucrose containing 0.5−50 μg ml−1 of one of the naturally occurring nectar alkaloids - caffeine, nicotine, senecionine, and gelsemine. Nectar uptake was determined by weighing each flower and comparing the weight to that of the control flower. Our experimental design minimized memorizing and marking; despite this, caffeine was significantly preferred at concentrations 0.5−2 μg ml−1 over control nectar; this preference was not observed for other alkaloids. All of the compounds tested were repellent at concentrations above 5 μg ml−1. We confirmed previous reports that bees exhibit a preference for caffeine, and hypothesize that this is not due only to addictive behaviour but is at least partially mediated by taste preference. We observed no significant preference for nicotine or any other alkaloid.

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Organo-Sulphur Garlic Compounds Influence Viability of Mammalian Cells: A Review

): Comparison of antioxidant activity of hydroethanolic fresh and aged garlic extracts and their effects on cerebral ischemia. Food Chemistry, 140, 343–352. doi: 10.1016/j.foodchem.2013.02.053. Chen LY, Chen Q, Zhu XJ, Kong DS, Wu L, Shao JJ, Zheng SZ (2016): Diallyl trisulfide protects against ethanol-induced oxidative stress and apoptosis via a hydrogen sulfide-mediated mechanism. International Immunopharmacology, 36, 23–30. doi: 10.1016/j.intimp.2016.04.015. Chen WC, Hsu SS, Chou CT, Kuo CC, Huang JK, Fang YC, Chang HT, Tsai JY, Liao WC, Wang BW, Shieh P, Kuo

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Effect of Transgenic Maize Mon 810 on Selected Non-Target Organisms: The Bird Cherry-Oat Aphid (Rhopalosiphum padi L.) and its Predator - Green Lacewing (Chrysoperla carnea Steph.)

P. M., Bigler F. 1998. Effects of transgenic Bacillus thuringiensis corn-fed prey on mortality and development time of immature Chrysoperla carnea ( Neuroptera: Chrysopidae ). Environmental Entomology 27 : 480-487. Hilbeck A., Moar W. J., Pusztai-Carey M., Filippini A., Bigler F. 1999. Prey-mediated effects of Cry1Ab toxin and protoxin and Cry2A protoxin on the predator Chrysoperla carnea. Entomologia Experimentalis et Applicata 91 : 305-316. Jepson P. C., Croft B. A., Pratt G. E. 1994. Test systems to

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Relevance of the Mi23 Marker and the Potato Aphid Biology as Indicators of Tomato Plant (Solanum lycopersicum L.) Resistance to Some Pests

-spotted spider-mite ( Tetranychus urticae Koch): Results of laboratory evaluation. Veget. Crops Res. Bull. 72:15-24. Goggin F. L., Williamson V. M., Ullman D. E. 2001. Variability in the response of Macrosiphum euphorbiae and Myzus persicae (Hemiptera: Aphididae) to the tomato resistance gene Mi. Environ. Entomol. 30: 101-106. [DOI: 10.1603/0046-225X-30.1.101] Goggin F. L., Gowri S., Williamson V. M., Ullman D. E. 2004. Developmental regulation of Mi -mediated aphid resistance is independent of Mi-1.2 transcript

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A Simple Dual Stain for Detailed Investigations of Plant-Fungal Pathogen Interactions

of potato late blight resistance mediated by the broad-spectrum resistance gene RB . Phytopathology 101: 263-270. [DOI: 10.1094/ PHYTO-04-10-0119] Currier H.B. 1957. Callose substance in plant cells. American Journal of Botany 44: 478-488. [DOI:10.2307/2438916] Diez-Navajas A.M., Greif C., Poutaraud A., Merdinoglu D. 2007. Two simplified fluorescent staining techniques to observe infection structures of the oomycete Plasmopara viticola in grapevine leaf tissues. Micron 38: 680-683. [DOI: 10.1016/j.micron.2006

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Effect Of Hydrolyzed Milk On The Adhesion Of Lactobacilli To Intestinal Cells

MG, Falvey E, Shanahan F, Cotter PD (2014): Exercise and associated dietary extremes impact on gut microbial diversity. doi: 10.1136/gutjnl-2013-306541. Coconnier MH, Klaenhammer TR, Kerneis S, Bernet MF, Servin AL (1992): Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucussecreting cell lines in culture. Applied and Environmental Microbiology, 58, 2034–2039. Collado MC, Meriluoto J, Salminen S (2008): Adhesion and aggregation properties of probiotic and pathogen strains. European Food Research and Technology

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Localization of Estrogen Receptors in Male Reproductive Tissues and Sperm Cells – A Review

30 and ERα. Molecular and Cellular Endocrinology, 320, 136–144. doi: 10.1016/j.mce.2010.01.035. Chimento A, Sirianni R, Zolea F, Bois C, Delalande C, Ando S, Maggiolini M, Aquila S, Carreau S, Pezzi V (2010b): GPER and ESRs are expressed in rat round spermatids and mediate oestrogen-dependent rapid pathways modulating expression of cyclin B1 and Bax. International Journal of Andrology, 34, 420–429. doi: 10.1111/j.1365-2605.2010.01100.x. Dostalova P, Zatecka E, Dvorakova-Hortova K (2017): Of oestrogen and sperm: a review of the roles of oestrogens and

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