Katarzyna Wojcicka-Lorenowicz, Krzysztof Kostro and Iwona Taszkun
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The influence of Pseudomonas aeruginosa secreted virulence factors on hemocyte-mediated immune response of Galleria mellonella
The influence of secreted virulence factors (SVF) of entomopathogenic strain of Pseudomonas aeruginosa on Galleria mellonella hemocytes-mediated immune response mechanisms was examined. The obtained results indicated that larvae injection with SVF adversely affected the morphology and consequently viability of hemocytes. Moreover, factors presented in culture supernatant lead to aberrant organization of the actin cytoskeleton. Within the time of SVF action, hemocytes preserved only the ability to adhere to the foreign surfaces in comparison to cells collected from naïve larvae, which formed pseudopodia and extensively spread. It seemed probable that cytoskeleton disruption led to overcoming hemocytes-mediated immune response mechanisms by entomopathogen. The phagocytosis in treated with SVF larvae was observed but hemocytes which engulfed bacteria became dead. Moreover, hemocytes ability to capsule formation was strongly inhibited in SVF-treated larvae.
Syed Ehtisham-ul-Haque, Madiha Kiran, Usman Waheed and Muhammad Younus
1. Aryan E., Makvandi M., Farajzadeh A., Huygen K., Bifani P., Mousavi S.L., Fateh A., Jelodar A., Gouya M.M., Romano M.: A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex. Microbiol Res 2010, 165, 211–220.
2. Ashraf W., Kamal H., Mobeen A., Waheed U., Unger H., Khan Q.: Loop-mediated isothermal amplification assay for rapid and sensitive detection of Peste des petits ruminants virus in field conditions. J Anim Plant Sci 2017, 27, 119–127.
Ramesh K. Saini, Muthu K. Akithadevi, Parvatam Giridhar and Gokare A. Ravishankar
- Isoflavone content in soybean seeds was enhanced by the elicitor-mediated approach under field conditions through the floral application of abiotic elicitors-salicylic acid, methyl jasmonate and biotic elicitors-Aspergillus niger and Rhizopus oligosporus. Among isoflavones, daidzein and glycitein were found to be highly responsive to elicitors, with an increase of 53.7% and 78.7%, respectively as compared to control. Highest total isoflavone content (1276.4 mg g-1 of seeds) was observed upon the administration of 0.1 mMsalicylic acid, which is 92.7% higher than in control. This study would be valuable for augmentation of the isoflavone content in soybean seeds in field grown plants for better nutraceutical potential.
Tomoki Hattori, Yang Chen, Shinichi Enoki, Daisuke Igarashi and Shunji Suzuki
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Eva Maria Prem, Nadine Praeg, Katrin Hofmann, Andreas Otto Wagner and Paul Illmer
C. Deppe M. 2012 Humic acid addition lowers methane release in peats of the Mer Bleue bog, Canada Soil Biology and Biochemistry 52 96 98
Boetius, A., Ravenschlag, K., Schubert, C.J., Rickert, D., Widdel, F., Gieseke, A., Amann, R., Jørgensen, B.B., Witte, U. and O. Pfannkuche (2000): A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407, 623. 10.1038/35036572 11034209 Boetius A. Ravenschlag K. Schubert C.J. Rickert D. Widdel F. Gieseke A. Amann R. Jørgensen B.B. Witte U
Małgorzata Pomorska-Mól, Iwona Markowska-Daniel and Jarosław Rachubik
Development of early immune response in piglets with subclinical swine influenza was investigated. Fourteen, seronegative piglets were used. Ten of them were infected intranasally with swine influenza virus (SIV) H1N1 subtype. Temperature and clinical signs were assessed daily. Leukocyte proportions and concentrations were analysed on a haematology analyser. Antibodies against SIVs were measured by haemagglutination inhibition assay. To measure influenza-specific cell-mediated immunity (CMI), the proliferation assay was performed. The real time reverse transcription PCR method was used for detection of SIV. No relevant respiratory or systemic clinical signs were observed. The presence of SIV RNA in nasal swabs from all infected piglets was confirmed between 2 and 5 dpi. The overall number of leukocytes did not differ during the study. The number of medium-sized cells (MID) was significantly higher on 2 and 4 dpi, as compared to day 0 level. The percentage of lymphocytes decreased from 74% on day 0 to 67.06% on 4 dpi, while the percentage of MID significantly increased at the same time. In control pigs no significant changes were observed. All infected pigs exhibited specific antibodies between 7 and 10 dpi. Specific CMI was observed before specific antibodies were present. Results of our research indicate that kinetics of the humoral and CMI response during subclinical infection is similar to that observed in clinical form of the disease.
Guanying Wang, Xinran Li, Renli Jiang, Yue Li, Xiaojing Fan, Yu Zheng and Li Gao
1. Caron J.P, Gandy J.C, Schmidt M., Hauptman J.G., Sordillo L.M.: Influence of corticosteroids on interleukin-1β stimulated equine chondrocyte gene expression. Vet Surg 2013, 42, 231-237.
2. Christgau S, Garnero P., Fledelius C, Moniz C, Ensig M, Gineyts E., Rosenquist C, Qvist P.: Collagen type II C-telopeptide fragments as an index of cartilage degradation. Bone 2001, 29, 209-215.
3. de Grauw J.C., Brama P.A, Wiemer P., Brommer H., van de Lest C.H., van Weeren P.R.: Cartilage-derived biomarkers and lipid mediators of inflammation in horses