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Specific cell-mediated immune response in rabbits immunised and infected with Trichophyton mentagrophytes

References 1. Banks K.L., Clarkson T.B.: Naturally occurring dermatomycoses in rabbit. J Am Vet Med Assoc 1967, 151, 926-929. 2. Bendixen G., Soborg H.: A leucocyte migration technique for in vitro detection of cellular (delayed type) hypersensitivity in man. Danish Med Bull 1969, 16, 1-8. 3. Calderon R.A., Hay R.J.: Cell-mediated immunity in experimental murine dermatophytosis. II. Adoptive transfer of immunity to dermatophyte infection by lymphoid cells from donors with acute or chronic

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The influence of Pseudomonas aeruginosa secreted virulence factors on hemocyte-mediated immune response of Galleria mellonella

The influence of Pseudomonas aeruginosa secreted virulence factors on hemocyte-mediated immune response of Galleria mellonella

The influence of secreted virulence factors (SVF) of entomopathogenic strain of Pseudomonas aeruginosa on Galleria mellonella hemocytes-mediated immune response mechanisms was examined. The obtained results indicated that larvae injection with SVF adversely affected the morphology and consequently viability of hemocytes. Moreover, factors presented in culture supernatant lead to aberrant organization of the actin cytoskeleton. Within the time of SVF action, hemocytes preserved only the ability to adhere to the foreign surfaces in comparison to cells collected from naïve larvae, which formed pseudopodia and extensively spread. It seemed probable that cytoskeleton disruption led to overcoming hemocytes-mediated immune response mechanisms by entomopathogen. The phagocytosis in treated with SVF larvae was observed but hemocytes which engulfed bacteria became dead. Moreover, hemocytes ability to capsule formation was strongly inhibited in SVF-treated larvae.

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Real-time loop-mediated isothermal amplification (LAMP) of mgc2 gene of Mycoplasma gallisepticum

References 1. Aryan E., Makvandi M., Farajzadeh A., Huygen K., Bifani P., Mousavi S.L., Fateh A., Jelodar A., Gouya M.M., Romano M.: A novel and more sensitive loop-mediated isothermal amplification assay targeting IS6110 for detection of Mycobacterium tuberculosis complex. Microbiol Res 2010, 165, 211–220. 2. Ashraf W., Kamal H., Mobeen A., Waheed U., Unger H., Khan Q.: Loop-mediated isothermal amplification assay for rapid and sensitive detection of Peste des petits ruminants virus in field conditions. J Anim Plant Sci 2017, 27, 119–127. 3

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Augmentation of major isoflavones in Glycine max L. through the elicitor-mediated approach

Abstract

- Isoflavone content in soybean seeds was enhanced by the elicitor-mediated approach under field conditions through the floral application of abiotic elicitors-salicylic acid, methyl jasmonate and biotic elicitors-Aspergillus niger and Rhizopus oligosporus. Among isoflavones, daidzein and glycitein were found to be highly responsive to elicitors, with an increase of 53.7% and 78.7%, respectively as compared to control. Highest total isoflavone content (1276.4 mg g-1 of seeds) was observed upon the administration of 0.1 mMsalicylic acid, which is 92.7% higher than in control. This study would be valuable for augmentation of the isoflavone content in soybean seeds in field grown plants for better nutraceutical potential.

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Exogenous isoleucine and phenylalanine interact with abscisic acid-mediated anthocyanin accumulation in grape

. Integr. Genomics 10, 367-381. M ac D onald M.J., D’C unha G.B., 2007. A modern view of phenylalanine ammonia lyase. Biochem. Cell Biol. 85, 273-282. M atus J.T., L oyola R., V ega A., N eira A.P., B ordeu E., J ohnson P.A., et al ., 2009. Post-veraison sunlight exposure induces MYB-mediated transcriptional regulation of anthocyanin and flavonol synthesis in berry skins of Vitis vinifera . J. Exp. Bot. 60, 853-867. M ikami M., M ori D., M asumura Y., A oki Y., S uzuki S., 2017. Electrical stimulation: an abiotic stress generator for

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Toll-like receptor-4, but not toll-like receptor-2 mediates secretion of tumour necrosis factor α and interleukin-8 in lipopolysaccharide-stimulated mouse mammary epithelial cells

-475. 7. C. Gray Y.S., Donaldson L., Tellam R.L.: Bovine mammary epithelial cells, initiators of innate immune response to mastitis. Aust Exp Agricult 2005, 45 , 757-761. 8. Davies D., Meade K.G., Herath S., Eckersall P.D., Gonzalez D., White J.O., Conlan R.S., O'Farrelly C., Sheldon I.M.: Toll-like receptor and antimicrobial peptide expression in the bovine endometrium. Reprod Biol Endocrinol 2008, 6 , 53-64. 9. Dinarello C.A.: Cytokines as mediators in the pathogenesis of septic shock. Curr Top Microbiol Immunol 1996, 216 , 133

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Osmo and Hydro Priming Mediated Germination Improvement Under Cold Stress Conditions in Mountain Rye (Secale Montanum)

matric potential on emergence of cotton ( Gossypium hirsutum L.) and maize ( Zea mays L.). Soil and Tillage Research 74: 161-168. Oram R.N., 1996 - Secale montanum - a wider role in Australia? NZ J. Agric. Res 39: 629-633. Patade V.Y., Maya K., Zakwan A., 2011 - Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Res. J. Seed Sci 4 (3): 125 -136. Redmann R.E., 1974 - Osmotic and specific ion effect on the germination of alfalfa. Canadian Journal of

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Potential methane production and oxidation along the soil chronosequence of the Rotmoos glacier forefield

C. Deppe M. 2012 Humic acid addition lowers methane release in peats of the Mer Bleue bog, Canada Soil Biology and Biochemistry 52 96 98 Boetius, A., Ravenschlag, K., Schubert, C.J., Rickert, D., Widdel, F., Gieseke, A., Amann, R., Jørgensen, B.B., Witte, U. and O. Pfannkuche (2000): A marine microbial consortium apparently mediating anaerobic oxidation of methane. Nature 407, 623. 10.1038/35036572 11034209 Boetius A. Ravenschlag K. Schubert C.J. Rickert D. Widdel F. Gieseke A. Amann R. Jørgensen B.B. Witte U

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Development of Early Humoral and Cell-Mediated Immunity in Piglets with Experimentally Induced Subclinical Swine Influenza

Abstract

Development of early immune response in piglets with subclinical swine influenza was investigated. Fourteen, seronegative piglets were used. Ten of them were infected intranasally with swine influenza virus (SIV) H1N1 subtype. Temperature and clinical signs were assessed daily. Leukocyte proportions and concentrations were analysed on a haematology analyser. Antibodies against SIVs were measured by haemagglutination inhibition assay. To measure influenza-specific cell-mediated immunity (CMI), the proliferation assay was performed. The real time reverse transcription PCR method was used for detection of SIV. No relevant respiratory or systemic clinical signs were observed. The presence of SIV RNA in nasal swabs from all infected piglets was confirmed between 2 and 5 dpi. The overall number of leukocytes did not differ during the study. The number of medium-sized cells (MID) was significantly higher on 2 and 4 dpi, as compared to day 0 level. The percentage of lymphocytes decreased from 74% on day 0 to 67.06% on 4 dpi, while the percentage of MID significantly increased at the same time. In control pigs no significant changes were observed. All infected pigs exhibited specific antibodies between 7 and 10 dpi. Specific CMI was observed before specific antibodies were present. Results of our research indicate that kinetics of the humoral and CMI response during subclinical infection is similar to that observed in clinical form of the disease.

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Changes in Synovial Fluid Inflammatory Mediators and Cartilage Biomarkers After Experimental Acute Equine Synovitis

References 1. Caron J.P, Gandy J.C, Schmidt M., Hauptman J.G., Sordillo L.M.: Influence of corticosteroids on interleukin-1β stimulated equine chondrocyte gene expression. Vet Surg 2013, 42, 231-237. 2. Christgau S, Garnero P., Fledelius C, Moniz C, Ensig M, Gineyts E., Rosenquist C, Qvist P.: Collagen type II C-telopeptide fragments as an index of cartilage degradation. Bone 2001, 29, 209-215. 3. de Grauw J.C., Brama P.A, Wiemer P., Brommer H., van de Lest C.H., van Weeren P.R.: Cartilage-derived biomarkers and lipid mediators of inflammation in horses

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