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Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification

H., Long B., Wu X., Bai Y., Development of a Loop-Mediated Isothermal Amplification Assay for sensitive and rapid detection of Cronobacter sakazakii. Foodborne Pathog. Dis., 2012, 9, 1111-1118. 11. Francois P., Tangomo M., Hibbs J., Bonetti E.J., Boehme C.C., Notomi T., Perkins M.D., Schrenzel J., Robustness of a loopmediated isothermal amplification reaction for diagnostic applications. FEMS Immunol. Med. Microbiol., 2011, 62, 41-48. 12. Fratamico P.M., Bagi L.K., Cray W.C. Jr., Narang N., Yan X., Medina M., Liu Y., Detection by

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Does SIRT-1 Mediate Calorie Restriction and Prolong Life? – A Mini Review

Abstract

Calorie restriction is the only intervention proved to prolong both average and maximum lifespan in yeast, worms, fish, rodents and possibly primates. Not only does the regimen prolong life, but it also reduces the incident of numerous age-related diseases like diabetes, atherosclerosis or cancer and slows down ageing. Mechanisms by which that is thought to occur have not yet been elucidated, but they probably involve reactive oxygen species signaling, insulin growth factor and transcriptional factors. Here, special emphasis is given to SIRT1 - silent information regulator. There is sound evidence showing that SIRT1 is a key player in mediating physiological response to calorie restriction and that its overexpression is correlated with extended lifespan. The possible mechanism leading to its elevated levels is high NAD/NADH ratio, observed in Sir2 in yeast. SIRT1 increases glucose production, enhances fat mobilization, stimulates angiogenesis, prevents neuronal degeneration and rises insulin sensitivity. Therefore, it seems to be a very beneficial factor activated by such a simple intervention that is calorie restriction.

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A regression approach to estimate the relative roles of pollen- versus seed-mediated gene flow under an isolation by distance model

, Guesnet D, Demesure B (2001) Pol­len- versus seed-mediated gene flow in a scattered forest tree species. Evo­lution 55(6): 1123-1135. https://doi.org/10.1554/0014-3820(2001)055[1123:pvsmgf]2.0.co;2 Rousset F (1997) Genetic differentiation and estimation of gene flow from F-sta­tistics under isolation by distance. Genetics 145: 1219-1228. Rousset F (2008) Genepop’007: a complete reimplementation of the Genepop software for Windows and Linux. Mol Ecol Res 8: 103-106. https://doi.org/10.1111/j.1471-8286.2007.01931.x

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Agrobacterium tumefaciens-mediated Transformation of Jatropha curcas: Factors Affecting Transient Transformation Efficiency and Morphology Analysis of Transgenic Calli

References GODWIN, I., G. TODD, B. FORD-LLOYD and H. J. NEWBURY (1991): The effects of acetosyringone and pH on Agrobacterium-mediated transformation vary according to plant species. Plant Cell Rep 9: 671-675. HÄSLER, J., J. WÜEST, T. GASPAR and M. CRÈVECOEUR (2003): Long term in vitro-cultured plant cells show typical neoplastic features at the cytological level. Biol Cell 95: 357-364. HIEI, Y., S. OHTA, T. KOMARI and T. KUMASHIRO (1994): Efficient transformation of rice (Oryza sative L.) mediated by

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Variations in the expression pattern of HSP27 and MSK1 genes during the development of prehierarchical follicles in the Zi geese (Anser cygnoides)

References Brami-Cherrier K., Roze E., Girault J.A., Betuing S., Caboche J. (2009). Role of the ERK/MSK1 signalling pathway in chromatin remodelling and brain responses to drugs of abuse. J Neurochem., 108: 1323-1335 Cargnello M., Roux P.P. (2012). Activation and function of the MAPKs and their substrates, the MAPK-activated protein kinases. Microbiol. Mol. Biol. Rev., 76: 496. Carpenter O.L., Wu S. (2014). Regulation of MSK 1-Mediated NF-κB Activation Upon UVB Irradiation. Photochem. Photobiol., 90: 155-161. Deak M., Clifton A.D., Lucocq

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Mechanism and functions of identified miRNAs in poultry skeletal muscle development

, H., Qin, Z.S., Carter-Su, C., Streeter, C., 2011. C/EBPβ Mediates Growth Hormone-Regulated Expression of Multiple Target Genes. Mol. Endocrinol. 25, 681–693. https://doi.org/10.1210/me.2010-0232 Cutting, A.D., Bannister, S.C., Doran, T.J., Sinclair, A.H., Tizard, M.V.L., Smith, C.A., 2012. The potential role of microRNAs in regulating gonadal sex differentiation in the chicken embryo. Chromosom. Res. 20, 201–213. https://doi.org/10.1007/s10577-011-9263-y Darnell, D.K., Kaur, S., Stanislaw, S., Konieczka, J.K., Yatskievych, T.A., Antin, P.B., 2006

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The role of endocannabinoid system based on mRNA expression during the late luteal phase and estrus in the bovine endometrium

). Endocannabinoids as endometrium inflammatory markers in lactating Holstein cows. Reprod Dom Anim, 53(3), 769-775. Bradshaw, H.B., Rimmerman, N., Krey, J.F., Walker, J.M. (2006). Sex and hormonal cycle differences in rat brain levels of pain-related cannabimimetic lipid mediators. American Am J Physiol Regul Integr Comp Physiol. 291, 349–358. Demuth, D.G., Molleman, A. (2006). Cannabinoid signalling. Life Science. 78, 549–563 Beltramo, M., Stella, N., Calignano, A., Lin, S.Y., Makriyannis, A., Piomelli, D. (1997). Functional role of high-affinity anandamide

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Garlic Sulfur Compounds Suppress Cancerogenesis and Oxidative Stress: a Review

-3278(96)90061-7. Benavides GA, Squadrito GL, Mills RW, Patel HD, Isbell TS, Patel RP, Darley-Usmar VM, Doeller JE, Kraus DW (2007): Hydrogen sulfide mediates the vasoactivity of garlic. Proceedings of the National Academy of Sciences of the United States of America, 104, 17977-17982. doi: 10.1073/ pnas.0705710104. Block E, Ahmad S, Jain MK, Crecely RW, Apitzcastro R, Cruz MR (1984): (E,Z)-ajoene - a potent antithrombotic agent from garlic. Journal of the American Chemical Society, 106, 8295-8296. doi: 10.1021/ja00338a049. Borek C (2001): Antioxidant health

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A review of ascorbic acid potentialities against oxidative stress induced in plants

-16295. Jiang L, Wang Y, Bjorn LO, Li S (2011): UV-B-induced DNA damage mediates expression changes of cell cycle regulatory genes in Arabidopsis root tips. Planta 233: 831-841. Koca H, Bor M, Ozdemir F, Turkan I (2007): The effect of salt stress on lipid peroxidation, antioxidative enzymes and proline content of sesame cultivars. Environ Exptl Bot 60: 344-351. Kumari R, Singh S, Agrawal SB (2010): Response of ultraviolet-B induced antioxidant defense system in a medicinal plant, Acorus calamus. J Environ Biol 31: 907

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Repellence and attraction of Apis mellifera foragers by nectar alkaloids

Abstract

Plant secondary metabolites present naturally in nectar, such as alkaloids, may change the behavioural responses of floral visitors and affect pollination. Some studies have shown that nectar containing low concentrations of these secondary metabolites is preferred by honey bee foragers over pure nectar. However, it remains unclear whether this is caused by dependence or addictive behaviour, a simple taste preference, or by other conditions such as self-medication. In our choice experiment, free-flying bees were presented with artificial flowers holding 20% sucrose containing 0.5−50 μg ml−1 of one of the naturally occurring nectar alkaloids - caffeine, nicotine, senecionine, and gelsemine. Nectar uptake was determined by weighing each flower and comparing the weight to that of the control flower. Our experimental design minimized memorizing and marking; despite this, caffeine was significantly preferred at concentrations 0.5−2 μg ml−1 over control nectar; this preference was not observed for other alkaloids. All of the compounds tested were repellent at concentrations above 5 μg ml−1. We confirmed previous reports that bees exhibit a preference for caffeine, and hypothesize that this is not due only to addictive behaviour but is at least partially mediated by taste preference. We observed no significant preference for nicotine or any other alkaloid.

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