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Lucian Negura and Anca Negura


Both incidence and mortality of colorectal cancer (CRC) in Romania have shown a continuous increase during the last decades. Hereditary Non-Polyposic Colorectal Cancer (HNPCC), also known as Lynch syndrome, is mainly attributable to mismatch repair (MMR) genes MSH2, MSH6, and MLH1. Individuals carrying germ-line mutations of these genes present high lifetime risk of colorectal and other cancers, compared to non-carriers. Oncogenetics is developed worldwide nowadays, for identifying hereditary predisposition to cancer and offering appropriate clinical follow-up to patients and mutation carriers in Lynch families. Molecular oncogenetic diagnosis in Lynch syndrome is based on complete Sanger sequencing of entire MMR genes, which is time and resources consuming, therefore needing an appropriate and adapted optimization. Conventional sequencing requires a sufficient number of available samples to be processed simultaneously, which increases the waiting time for diagnostic results. Complete analysis for only one patient meets difficult technical problems due to the complex co-amplification of all gene regions of interest within the same conditions, therefore increasing the costs and reducing the cost-effectiveness of the test. Here we present an original and robust technical protocol for sequencing the entire MSH2, MSH6, and MLH1 coding sequence for one patient in a single PCR plate. Our optimized and verified system overcomes all technical problems and offers a quick, robust, and cost-effective possibility to personalize molecular oncogenetic diagnosis in Lynch syndrome.

Open access

V. Doničová, A. Lukačínová, R. Beňačka and F. Ništiar

. Toxicol. Oncol. , 33 (3), 183—194. DOI: 10.1615/JEnvironPatholToxicolOncol.2014011075. 18. Lukacinova, A., Benacka, R., Sedlakova, E., Lovasova, E., Nistiar, F., 2012: Multigenerational lifetime low-dose exposure to heavy metals on selected reproductive parameters in rats. J. Environ. Sci. Health A Tox. Hazard Subst. Environ. Eng. , 47 (9), 1280—1287. DOI: 10.1080/10934529.2012.672132. 19. Lukačínová, A., Rácz, O., Lovásová, E., Ništiar, F., 2011: Effect of lifetime low dose exposure to heavy metals on selected serum proteins of Wistar rats during three

Open access

Songfa Zhang, Shan Jiang and Xiao Zang

vaccine. To achieve the 80% aim, governments and scientists should work together to increase the awareness of HPV-related diseases. It is crucial to make the population aware that every person is likely to be infected by HPV in his/her lifetime, and that HPV infection is associated with many diseases. Other measures include providing coverage in health insurances and making the HPV vaccination a mandated program for children. Acknowledgments We thank our colleagues from Zhejiang Women’s Hospital, University of British Columbia, and Simon Fraser University. The

Open access

Paulina Kobak and Bogumiła Pilarczyk

-462. 13. Hilgenstock F., Hamann H., Rosenberge E., Götz K.U., Distl O.: Analysis of health traits in different lifetime classes in stationary progeny tested German Fleckvieh bulls. Arch Tierz 2006, 49 , 222-223. 14. Iqbal M.U., Sajid M.S., Hussain A., Khan M.K.: Prevalence of helminth infections in dairy animals of nestle milk collection areas of Punjab (Pakistan). Ital Anim Sci 2007, 6 , 935-938. 15. Jiang S.X., Bayón J.E., Ferre I., Mao X.Z., González- Gallego J.: Effect of experimental fascioliasis on antipyrine metabolism and

Open access

Roberto Tocci, Clara Sargentini, Andrea Martini, Luisa Andrenelli, Antonio Pezzati, Doria Benvenuti and Alessandro Giorgetti

A.: Differentiation between fore and hind hoof dimensions in the horse (Equus caballus). Arch Tierz 2008, 51, 531-540. 27. Stachurska A., Walkuska G., Cebera M., Jaworski Z., Chalabis- Mazurek A.: Heavy metal status of Polish Konik horses from stable-pasture and outdoor maintenance systems in the Masurian environment. J Elementol 2011, 16, 623-633. 28. Strasser H.: A lifetime of soundness, Ed & Trans. Sabine Kell, Qualicum Beach BC, Canada 2000. 29. Tocci R., Sargentini C., Benedettini A., Benvenuti D., Pezzati A

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Margit Șerban, Dan Poenaru, Laura Cernat, Delia Savescu, Jenel Pătrașcu, Wolfgang Schramm, Emilia Ursu, Delia Mihailov, Cristian Jinca, Ioana Ioniță and Smaranda Arghirescu

/3 multicenter clinical trial in severe hemophilia A. Blood. 2016; 128 (5): 630-637. DOI: 10.1182/blood-2016-01-687434 16. Sorensen B, Ingerslev J. Tailoring haemostatic treatment to patient requirements - an update on monitoring haemostatic response using thrombelastography. Haemophilia. 2005; 11 (1):1-6. DOI: 10.1111/j.1365-2516.2005.01156.x 17. Négrier C, Gomperts ED, Oldenburg J. The history of FEIBA: a lifetime of success in the treatment of haemophilia complicated by an inhibitor. Haemophilia. 2006;12:4-13. DOI: 10.1111/j.1365

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Monika Marczak, Krystyna Marta Okoniewska, Jakub Okoniewski, Tomasz Grabowski and Jerzy Jan Jaroszewski

surviving fraction. Lifetime Data Anal 2007, 13, 351–369. 26. Piatkowska M., Jedziniak P., Zmudzki J.: Review: Residues of veterinary medicinal products and coccidiostats in eggs – causes, control and results of surveillance program in Poland. Pol J Vet Sci 2012, 15, 803–812. 27. Pratim R.P., Paul S., Mitra I., Roy K.: On two novel parameters for validation of predictive QSAR models. Molecules 2009, 14, 1660–1701. 28. Tetko I.V., Gasteiger J., Todeschini R., Mauri A., Livingstone D., Ertl P., Palyulin V.A., Radchenko E.V., Zefirov N.S., Makarenko A

Open access

Ryota Masuzaki and Masao Omata

States. Hepatology 1993;18:1326-1333. 31 Tanaka K, Hirohata T, Takeshita S, Hirohata I, Koga S, Sugimachi K, et al. Hepatitis B virus, cigarette smoking and alcohol consumption in the development of hepatocellular carcinoma: a case-control study in Fukuoka, Japan. Int J Cancer 1992;51:509-514. 32 Donato F, Tagger A, Gelatti U, Parrinello G, Boffetta P, Albertini A, et al. Alcohol and hepatocellular carcinoma: the effect of lifetime intake and hepatitis virus infections in men and women. Am J Epidemiol 2002;155:323-331. 33

Open access

Soo-Hyeon Kim, Byung-Joon Seung, Seung-Hee Cho, Ha-Young Lim, Hee-Myung Park and Jung-Hyang Sur

tissues ( 14 ). Based on these findings, it could be hypothesised that androgen stimulates normal perianal gland continuously to spontaneously change into preneoplastic lesion or adenoma during the dog’s lifetime. Consequently, adenoma mainly occurs in intact male dogs. On the other hand, dogs are castrated at different times in their life, and induced preneoplastic lesion or adenoma returns to a normal-like perianal gland, thus perianal gland adenoma does not occur. However, if another molecular change - such as p63 - stimulates the perianal gland, malignant