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Julia Choina, Diana Dolat, Ewelina Kusiak, Magdalena Janus and Antoni Morawski
Melda Altikatoglu and Huriye Kuzu
Improvement of enzyme stability via non-covalent complex formation with dextran against temperature and storage lifetime
The optimal methodology to prepare the novel modified enzyme, polymer-enzyme complex, was developed to give a high catalytic activity in aqueous solution. The non-covalent complexes of two different enzymes (horseradish peroxidase and glucose oxidase) were prepared with various molar ratios (nD/nE 0,05; 0,1; 1; 5; 10; 15; 20) by using 75kDa dextran. The thermal stabilities of the obtained complexes were evaluated with the activities determined at different temperatures (25, 30, 35, 40, 50, 60, 70, 80°C) applying 60 minutes incubation time for pH 7. The complexes with the molar ratio nD/nHRP: 10 and nD/nGOD: 5 showed the highest thermal stability. Its activity was very high (ca. 1,5-fold higher activity than pure enzyme for HRP-dextran complexes) and almost the same between applying one hour incubation time and without incubation, and could also be measured at high temperatures (70, 80 °C). We finally succeeded in preparing dextran-enzyme complexes which showed higher activity than pure enzyme in aqueos solution at all temperatures for pH 7. In addition, the mentioned complexes at pH 7 had very long storage lifetime compared to purified enzyme at +4 °C; which is considered as a good feature for the usage in practice.
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Monika Wałęsa-Chorab, Adam Gorczyński, Damian Marcinkowski, Zbigniew Hnatejko and Violetta Patroniak
photocatalytic properties of new dinuclear helical complex of silver(I) ions. J. Catal . 291, 1-8. DOI: 10.1016/j.jcat.2012.03.025. 14. Yam, V.W.W., Chan, Kenneth, H.Y., Wong, Keith, M.C. & Zhu, N. (2005). Luminescent platinum(II) terpyridyl complexes: effect of counter ions on solvent-induced aggregation and color changes. Chem. Eur. J. 11, 4535-4553. DOI: 10.1002/ chem.200500106. 15. Ji, S., Wu, W., Wu, W., Song, P., Han, K., Wang, Z., Liu, S., Guo, H. & Zhao, J. (2010). Tuning the luminescence lifetimes of ruthenium
Muhammad Waqar Ashraf
lifetime of supported liquid membrane. J. Memb. Sci., 133, 269–273. DOI: 10.1016/S0376-7388(97)00083-5. 21. Yang, X.J. & Fane, A.G. (1999). Performance and stability of support liquid membrane using LIX 984N for copper transport. J. Memb. Sci. 156, 251–263. DOI: 10.1016/S0376-7388(98)00351-2. 22. Ferriera, F.C., Shejiao, H. & Livingston, G. (2002). Recovery of aniline from aqueous solution using the membrane aromatic recovery system (MARS). Ind. Eng. Chem. Res. 41, 2766. DOI: 10.1021/ie010746l.
Paulina Rokicka, Agata Markowska-Szczupak, Łukasz Kowalczyk, Ewa Kowalska and Antoni W. Morawski
of the bacterial interaction with visible-light responsive titania photocatalyst on the bacteridical performance. J. Biom. Sci. 16(1), 7. DOI: 10.1186/1423-0127-16-7. 28. Choina, J., Dolat, D., Kusiak, E., Janus, M. & Morawski, A.W. (2009). TiO 2 modified by ammonia as a long lifetime photocatalyst for dyes decomposition. Pol. J. Chem. Technol. 11(4), 1–6. DOI: 10.2478/v10026-009-0035-9. 29. Bubacz, K., Choina, J., Dolat, D. & Morawski, A.W. (2010). Methylene blue and phenol photocatalytic degradation on nanoparticles of anatase TiO 2 . Pol. J. Environ
Wei Li, Jie Ren, Xiao-Yan Zhao and Takayuki Takarada
carbon nanotubes. Pol. J. Chem. Technol. 19, 61-67. DOI: 10.1515/pjct-2017-0050. 24. Ren, J., Cao, J.P., Zhao, X.Y., Wei, F., Zhu, C. & Wei, X.Y. (2017). Extending catalyst lifetime by doping of Ce in Ni loaded on acid-washed lignite char for biomass catalytic gasifi cation. Catal. Sci.Technol. 7, 5741-5749. DOI: 10.1039/C7CY01670K. 25. Zeng, Y., Ma H.F., Zhang, H.T., Ying, W.Y. & Fang, D.Y. (2014). Impact of heating rate and solvent on Ni-based catalysts prepared by solution combustion method for syngas methanation. Pol. J. Chem
Tibor Teplicky, Dusan Chorvat, Miroslav Michalka and Alzbeta Marcek Chorvatova
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