The main objectives of the present project are to study and to improve mechanical properties of different systems from mechatronic and biomedical domains, in order to increase their functionality and life span. This is why nanostructured thin films (e.g. Al, Cr, Ti, Ti/Al multilayers) were deposited on different steel substrates, used in mechatronic and biomedical applications. By the characterization of coated surfaces of the products used in various fields such as medicine, mechatronics, electronics, etc. depends their proper operation, durability and reliability. This is the main reason why, we studied new types of layers and multilayers using Atomic Force Microscopy and scratch tests. The main result of the realized tests is that all studied nanostructured thin films offer the possibility of increasing the lifetime of substrates, being an important factor for proper functional operation, durability and reliability of the final systems in which they are used.