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Darja Pavlin, Maja Cemazar, Andrej Cör, Gregor Sersa, Azra Pogacnik and Natasa Tozon

: 698-707. DelVecchio M, Bajetta E, Canova S, Lotze MT, Wesa A, Parmiani G, et al. Interleukin-12: biological properties and clinical application. Clin Cancer Res 2007; 13 : 4677-85. Heinzerling LM, Feige K, Rieder S, Akens MK, Dummer R, Stranzinger G, et al. Tumor regression induced by intratumoral injection of DNA coding for human interleukin 12 into melanoma metastases in gray horses. J Mol Med 2001; 78 : 692-702. Siddiqui F, Li C-Y, LaRue SM, Poulson JM, Avery PR, Pruitt AF, et al

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Jozko Racnik, Tanja Svara, Marko Zadravec, Mitja Gombac, Maja Cemazar, Gregor Sersa and Natasa Tozon

response of ECT with cisplatin or bleomycin in tumours of different histology with minimal side effects in dogs 10 , 11 , cats 12 , horses 13 , pet rats 14 , and reptiles 15 , 16 . In our cases, only some muscle contractions of the patient were observed during the application of electric pulses. The contractions were mild, disappearing after the end of each train of electric pulses. In animals, pain during ECT can be avoided using sedation and/or general anaesthesia. 10 , 11 , 12 , 13 , 14 , 15 , 16 Gaseous anaesthesia with isoflurane was used in our cases to prevent

Open access

Claudia Salvadori, Tanja Svara, Guido Rocchigiani, Francesca Millanta, Darja Pavlin, Maja Cemazar, Ursa Lampreht Tratar, Gregor Sersa, Natasa Tozon and Alessandro Poli

lasting disease free interval (between 3 and 4 years). Furthermore the present study immunohistologically supports the value of peritumoral IL-12 gene electrotransfer in boosting immune response of the organism, providing evidence of the presence of immune cells (T-lymphocytes and macrophages) in the tumors treated with electrochemotherapy and peritumoral IL-12 gene electrotransfer compared to tumors before therapy. There is also another study on sarcoids in horses, using the same combined treatment in which increased number of CD4 and CD8 lymphocytes sub