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Peng Zhang, Chunli Liu, Shanshan Song and Chifang Peng

Abstract

A novel method of immobilizing horse radish peroxidase enzyme (HRP) onto the surface of gold nanoparticles (GNPs) was developed. As a result, a high-activity bionanoconjugates was obtained through utilizing the biotin-streptavidin (SA) system. The HRP-SA-GNP bionanoconjugate with high activity was conveniently prepared through the biotin- avidin system. Compared with the HRP-GNP bioconjugate prepared through the traditional electrostatic absorption method, the enzyme activity per GNPs of this new bionanoconjugate was enhanced by 10 times. Moreover, the enzyme activity of this bionanoconjugate was controllable. The above method of bionanoconjugation preparation has promising applications in the fields including preparing highly active bio-nanoprobe and immobilized enzyme.

Open access

Ali Parsa and Maryam Tajik

on p-phenylenediamine functionalized glassy carbon electrode and its use for the direct electrochemistry of horse heart cytochrome c. Electrochim. Acta 55(3), 641–647. DOI: 10.1016/j.electacta.2009.09.017. 28. Shimano, J.Y. & MacDiarmid, A.G. (2001). Polyaniline, a dynamic block copolymer: Key to attaining its intrinsic conductivity? Synth. Met. 123(2), 251–262. DOI: 10.1016/S0379-6779(01)00293-4. 29. Genies, E.M. & Lapkowski, M. (1988). Polyaniline films. Electrochemical redox mechanisms. Synth. Met. 24(1–2), 61–68. DOI: 10

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Peter Pristas, Jana Kiskova, Ivana Timkova, Lenka Malinicova, Alena Luptakova, Maria Kusnierova and Jana Sedlakova-Kadukova

Abstract

The genus Acidithiobacillus comprises 7 species of Gram-negative obligatory acidophilic chemolithotrophic bacteria that derive energy mainly from the oxidation of reduced sulphur compounds. Four of the species also catalyse the dissimilatory oxidation of ferrous iron while three (A. thiooxidans, A. albertensis, and A. caldus) do not. Bacteria from the genus Acidithiobacillus are often associated with mineral biotechnologies (biomining) and acid mine drainage. While acceleration of mineral solubilisation is a positive aspect in environmental biotechnologies, it is undesirable in acid mine drainage with strong negative ecological impact and there is profound interest in genetics and genomics of these bacteria. Representatives of Acidithiobacillus genus occur world-wide, however there are limited data on Acidithiobacillus spp. variability from Slovakia. In our work the variability of Acidithiobacillus spp., from Slovakia was analysed and the presence of A ferrooxidans was detected. In addition, for the first time we report here on the occurrence of A. albertensis as well. Comparative analyses confirmed pronounced genetic and genomic diversity within the genus, especially within A. ferrooxidans and A. thioxidans complexes. Based on data presented, several Acidithiobacillus species could be considered as a complex species and the description of several new species is very probable in the near future.

Open access

Jaroslav Vatrál and Roman Boča

.N.L., LINERT, W.: Iron release from horse-spleen ferritin by 6- hydroxydopamine : A kinetic study. J. Inorg. Biochem., 67, 1997, 164-164. KAMYABI, M.A., AGHAJANLOO, F.: Electrocatalytic response of dopamine at a carbon paste electrode modified with ferrocene. Croat. Chem. Acta, 82(3), 2009, 599-606. LLOYD, R.V., HANNA, P.M., MASON, R.P.: The origin of the hydroxyl radical oxygen in the Fenton reaction. Free Rad. Biol. Med., 22, 1997, 885-888. SAYRE, L.M., PERRY, G., HARRIS, P.L.R., LIU, Y., SCHUBERT, K.A., SMITH, M.A.: In Situ