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Gang Lin, Xiaoyan Duan, Xiaobo Cai, Liyan Tian, Zhengjie Xu and Jiangao Fan

Abstract

Background: Non-alcoholic fatty liver disease is considered a hepatic manifestation of the metabolic syndrome. It is associated with endothelial dysfunction as an early event of generalized atherosclerosis. However, it is unclear whether steatotic hepatocytes influence endothelial function directly. Objective: Explore the influence of hepatocyte steatosis on the function of endothelial cells. Methods: Oleic and palmitic acid (2:1 mixture, final concentration: 1 mM for 24 hours) was used to induce a normal adult hepatocyte strain (L-02) for transformation into steatosis cells. This was followed by oil red O staining and transmission electron microscopy (TEM) for verification. The culture solution of steatotic L-02 cells was filtered and collected, and added into the culture substrate of human umbilical vein endothelial cells (HUVECs). The expression of vascular cellular adhesion molecule -1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-1), and E-selectin in HUVECs was detected by real-time polymerase chain reaction and Western blot assays. The apoptosis and proliferation of HUVECs was determined using flow cytometry. The experimental results were compared with the controls. Results: Oil red O staining and microscopic observation showed that the cytoplasm of induced L-02 cells contained a large amount of red lipid droplets. TEM results showed that the cytoplasm had lipid accumulation, swelling mitochondria, fewer cristae, and reduced number of rough endoplasmic reticula accompanied with degranulation. However, these changes were not observed in normal L-02 cells. As to the group of HUVECs treated by the filtrate of steatosis L-02 cells, the mRNA and protein expression of VCAM-1, ICAM-1, and E-selectin was higher than that in the control group. The difference was statistically significant (p <0.01). No significant difference was found when HUVECs apoptosis and proliferation were assessed by flow cytometry. Conclusion: Secretion from steatotic hepatocytes could boost the expression of VCAM-1, ICAM-1, and E-selectin in endothelial cells, indicating that hepatocyte steatosis could induce endothelial cell dysfunction. The proliferation and apoptosis of endothelial cells did not change, suggesting that hepatocyte steatosis had no influence on the viability of endothelial cells under this condition.

Open access

Panu Praphatsorn, Duangporn Thong-Ngam, Onanong Kulaputana and Naruemon Klaikeaw

:757-61. 14. Yano H, Kinoshita S, Yano L. Acute exercise induces mitocondria swelling of hepatocytes surrounding the terminal hepatic venule in rat liver acinus. Jpn J Phys Fitness Sports Med. 1997; 46:49-54. 15. Latour MG, Brault A, Huet PM, Lavoie JM. Effects of acute physical exercise on hepatocyte volume and fuction in rat. Am J Physiol. 1999; R1258-64. 16. Yano L, Yano H, Takeda K. Electromagnetic determination of portal venous flow in rats during exercise. Inter Hepato Commun. 1996; 5:184-90. 17. Fojt E, Ekelund L

Open access

Natthaporn Tanpowpong and Teerasak Phewplung

Abstract

Background: Gadolinium diethylenetriamine-pentaacetic acid (Gd-EOB-DTPA) is a developed agent with preferential uptake by hepatocytes. A rapid and specific hepatocyte uptake with biliary excretion was observed of approximately 50% of the injected dose. The amount of contrast uptake is thought to be related to reserve liver function.

Objectives: To evaluate correlation between liver signal intensity in the hepatobiliary phase of Gd-EOB-DTPA and reserved liver function by using the model score for end-stage liver disease (MELD).

Methods:All patients who underwent magnetic resonance (MR) imaging with Gd-EOB-DTPA were retrospectively collected. The patients with serum creatinine level higher than 1.5 mg/dL or patients without available data to estimate MELD score were excluded. Thirty-six patients were enrolled. A signal-to-noise ratio (SNR) in the liver parenchyma on a fat-suppressed three dimensional fast spoiled-gradient recalled echo sequence images before and 20 minutes after contrast injection were measured and calculated on PACS by two radiologists. The MELD score was determined and interobserver reliability was estimated.

Results: Among 36 patients, we found a negative relationship between the percentage enhancement and the MELD score (P < 0.01, r = 0.545). The SNR at 20 minutes after Gd-EOB-DTPA injection also had a negative relationship with the MELD score with statistical significance (P < 0.01, r = 0.460). Interobserver reliability was 0.675.

Conclusion: The percentage enhancement in hepatobiliary phase of Gd-EOB-DTPA can predict reserved liver function.

Open access

Boonchoo Sirichindakul, Virote Sriuranpong, Naruemon Wisedopas, Bunthoon Nonthasoot, Jade Suphapol and Supanit Nivatvongs

Abstract

Background: Severe clinical hepatitis after imatinib treatment has been reported anecdotally. Hepatic tissue of patients with liver matastasis is often fragile and difficult to handle during liver resection from gastrointestinal stromal tumor (GIST).

Objective: Observe hepatic tissue of these patients and examine the detailed histopathology underlying the change in the texture of non-tumorous hepatic parenchyma of these patients.

Materials and methods:We reviewed six GIST patients with liver metastases who underwent hepatic resection at King Chulalongkorn Memorial Hospital between July 2004 and November 2005. Four patients did not have imatinib and two patients received imatinib for four and eight months before liver resection. Preoperative hepatic biochemistry profiles of all patients were unremarkable. We examined histopathology of non-tumorous hepatic parenchyma of these patients using H-E staining, and additional histochemistry for vascular endothelial growth factor and epidermal growth factor receptor using immunohistochemistry staining.

Results: In all patients, common histopathological changes were swelling of hepatocytes, diffuse parenchymal congestion, dilatation of central vein, and infiltration of portal tract by mononuclear cells. However, there was significant zone 3 hepatocytolysis only in patients who received imatinib treatment. Additionally, moderate degree of hepatic steatosis correlated well with the duration of imatinib exposure. Immunohistochemical study could not demonstrate any difference between these two groups.

Conclusion: In two cases of subclinical hepatotoxicity from exposure to imatinib, histopathologic findings were consistent with drug induced liver injury. Imatinib induced liver injury may be more common than obvious clinical hepatitis.

Open access

Wanvisa Udomsinprasert, Sittisak Honsawek, Wilai Anomasiri, Voranush Chongsrisawat, Paisarn Vejchapipat and Yong Poovorawan

? Eur J Gastroenterol Hepatol. 2003; 15:447. 5. Hu E, Liang P, Spiegelman BM. AdipoQ is a novel adipose-specific gene dysregulated in obesity. J Biol Chem. 1996; 271:10697-703. 6. Berg AH, Combs TP, Scherer PE. ACRP30/adiponectin: an adipokine regulating glucose and lipid metabolism. Trends Endocrinol Metab. 2002; 13:84-9. 7. Shimizu A, Takamura T, Matsuzawa N, Nakamura S, Nabemoto S, Takeshita Y, et al. Regulation of adiponectin receptor expression in human liver and a hepatocyte cell line. Metabolism

Open access

Sher Zaman Safi, Yasmin Badshah, Yasir Waheed, Kaneez Fatima, Sadia Tahir, Alamgir Shinwari and Ishtiaq Qadri

, Bakr I, Hosseiny ME, Hamid MA, Rekacewicz C, et al. Metabolic and cardiovascular risk profiles and hepatitis C virus infection in rural Egypt. Gut. 2007; 56:1105-10. 10. Asselah T, Boyer N, Guimont MC, Hatem DC, Tubach F , Nahon K, et al . Liver fibrosis is not associated with steatosis but with necroinflammation in French patients with chronic hepatitis C. Gut. 2003; 52:1638-43. 11. Qadri I, Iwahashi M, Kullak-Ublick GA, Simon FR. Hepatocyte nuclear factor (HNF) 1 and HNF4 mediate hepatic multidrug resistance protein 2 Up

Open access

Puth Muangpaisarn, Kanisa Jampoka, Sunchai Payungporn, Naruemon Wisedopas, Chalermrat Bunchorntavakul, Pisit Tangkijvanich and Sombat Treeprasertsuk

the hepatocytes without secondary hepatic fat accumulation. Exclusion criteria were the presence of other liver diseases (e.g., hepatitis Β or C infection, autoimmune hepatitis), significant alcohol consumption (more than 140 g per week in men and 70 g per week in women), decompensated cirrhosis or Child-Pugh score ≥7, receiving steatogenic medications within 6 months of enrollment, human immunodeficiency virus infection, malignancy, pregnancy, and contraindication to liver biopsy. The control group included healthy individuals without history of significant alcohol

Open access

Voranush Chongsrisawat, Paisarn Vejchapipat and Yong Poovorawan

biliary atresia. Pediatr Surg Int. 2004; 20:773-7. 6. Vejchapipat P, Theamboonlers A, Chaokhonchai R, Chongsrisawat V, Chittmittrapap S, Poovorawan Y. Serum hepatocyte growth factor and clinical outcome in biliary atresia. J Pediatr Surg. 2004; 39:1045-9. 7. Honsawek S, Chongsrisawat V, Vejchapipat P, Thawornsuk N, Tangkijvanich P, Poovorawan Y. Serum interleukin-8 in children with biliary atresia: relationship with disease stage and biochemical parameters. Pediatr Surg Int. 2005; 21:73-7. 8. Honsawek S, Chongsrisawat V

Open access

Yu Yen Ee and Chew Choy Hoong

-induced MUC5AC gene expression in human airway epithelial cells. Phytother Res. 2009; 23:1708-12. 7. Arai H, Uchida K, Nakamura K. Effect of ascorbate on acrolein modification of very low density lipoprotein and uptake of oxidized apolipoprotein E by hepatocytes. Biosci Biotech Bioch. 2005; 69:1760-2. 8. Garcia-Lafuente A, Guillamon E, Villares A, Rostagno MA, Martinez JA. Flavonoids as anti-inflammatory agents: Implications in cancer and cardiovascular disease. Inflamm Res. 2009; 58:537-52. 9. Wang Y, Moser AH, Shigenaga JK

Open access

Chris Donga, Jun Chen, Xiaoyan Zhang and Congjian Xu

:345-56. 10. Ogris M, Walker G, Blessing T, Kircheis R, Wolschek M, Wagner E. Tumor-targeted gene therapy: strategies for the preparation of ligand-polyethylene glycolpolyethylenimine/ DNA complexes. J Control Release. 2003; 91:173-81. 11. Sagara K, Kim SW. A new synthesis of galactose-poly (ethylene glycol)-polyethylenimine for gene delivery to hepatocytes, J Control Release. 2002; 79: 271-81. 12. Zheng W, Magid MS, Kramer EE, Chen YT. Folliclestimulating hormone receptor is expressed in human ovarian surface epithelium and fallopian tube