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Materials and methods
Collection, isolation and storage of Z. tritici
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and showed an in situ methane release. However, the diversity of methanotrophic microbial communities and the potential CH 4 oxidation increased with soil age; and 80- to 150-year-old soils were shown to take up methane ( Bárcena et al., 2010 , 2011). In previous batch experiments, it was shown that soils from the Rotmoos glacier foreland can produce and oxidize methane under certain conditions, thus showing that methanogens and methanotrophs can be present and active at these sites. However, soil age alone could not explain the alterations in potential methane
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Aquatic macroinvertebrates are widely used as bioindicators in stream ecosystems (e.g. WFD evaluation of ecological status of rivers) due to their abundance, diversity and range of responses to environmental stressors ( Hodkinson and