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Olga K. Savushkina, Elena B. Tereshkina, Tatiana A. Prokhorova, Irina S. Boksha, Denis S. Burminskii, Elena A. Vorobyeva, Margarita A. Morozova and Gulnur Sh. Burbaeva

°C. The resulting supernatant was diluted 5-fold in 50 mmol/L K-phosphate buffer, pH 7.4 ( 16 ). GDH activity measurement The activity of GDH was determined by a spectrophotometric method ( 19 ) with modifications ( 17 ). The sample volume added to 400 µL of the reaction mixture was 125 µL. The reaction mixture composition was 0.1 mmol/L NADH, 0.1 mol/L NH 4 Cl, 10 mmol/L α-ketoglutarate, 1 mmol/L ADP, and 2.6 mmol/L EDTA in 0.01 mol/L Tris-HCl pH 8.0 buffer. The measurement of the rate of reaction ( 1 ), in this case directed to the right, is based on the

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Yuliya I. Ragino, Viktoriya S. Shramko, Ekaterina M. Stakhneva, Elena I. Chernyak, Sergey V. Morozov, Elena V. Shakhtshneider, Yana V. Polonskaya, Liliia V. Shcherbakova and Alexander M. Chernyavskiy

-associated phospholipase A2 (Lp-PLA2), an enzyme whose increased level in the circulation is linked with a high risk of atherosclerotic-plaque destabilization, is being actively studied. Besides, this enzyme has been suggested to be a highly specific marker of intravascular inflammation ( 9 ). The present study was aimed at investigating the balance of FAs, the search for associations of FAs with oxidative-antioxidant parameters and oxidative-inflammatory biomarker LP-FLA2 in men with coronary atherosclerosis (CA) and coronary heart disease (CHD). Materials and Methods This

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Miliaela Ileana Ionescu, Adriana Z. Costache, Ovidiu Oniga, Horia L. Banciu and Iulia Lupan

. Structural characterization of Burkholderia pseudomallei adenylate kinase (Adk): profound asymmetry in the crystal structure of the 'open' state. Biochem Biophys Res Commun. 2010; 394(4):1012-7 7. Altschul SF, Madden TL, Schaffer AA, Zhang J, Zhang Z, Miller W, et al. Gapped BLAST and PSIBLAST: a new generation of protein database search programs. Nucleic Acids Res. 1997; 25(17): 3389-402 8. Thompson JD, Higgins DG, Gibson TJ. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting