Franziska Mairhofer, Norbert Barta, Pia Euteneuer, Julia Schuster, Alexander Bauer and Andreas Gronauer
on the result. The sampler (ST35 steel tube, 52 mm external diameter, 48 mm internal diameter, 2 mm wall thickness) was carefully hammered from above down to the plate. Before removing, the tube was rotated several times in order to cutoff outstanding straw. After removing the core and the surrounding soil, the plate was pulled out and driven in again, but 0.05 m deeper. This process was repeated for all segments. Repeated sharpening of the tube and the plate prevented the straw being pushed into the soil instead of being cut. After air drying of samples, crop
transformation rates. In general, liming promotes N fixation by the soil microbial community ( Holland et al., 2018 ). The effects on N mineralization are less clear-cut. N mineralization has been reported to increase, decrease, or remain unchanged ( Bailey et al 1995 ; Kemmitt et al., 2006 ; Wachendorf et al., 2015 ) but probably ameliorates with repeated lime applications ( Holland et al., 2018 ); however, if augmented, also availability for plant uptake increases and crop yields can benefit correspondingly, but N losses via nitrate leaching into groundwater can be spurred
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Ewa Babicz-Zielińska, Lidia Wądołowska and Dariusz Tomaszewski
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58. Krabbenborg M.A.M., Danner U.N., Larsen J.K., Van Der Veer N., Van Elburg A.A., De Ridder D.T.D., Evers C., Stice E., Engels R.C.M.E., The eating disorder diagnostic scale: Psychometric features within a clinical population and a cut-off point to differentiate clinical patients from healthy controls. Eur. Eat. Disord. Rev., 2012, 20, 315-320.
59. Kratina K
I. Christiana, R. Aparnaa, R. Rohit, D. Nageswara Rao and P. Kaliraj
periodically after immunization and stored at -80 °C and the antibody titer was determined by ELISA, in which 96-well microtiter plates (Immulon2, Dynatech, VA, USA) were coated with 100 ng/well of respective antigen. The plates were blocked with 5 % skimmed milk, after washing with PBST and PBS, a 2 fold serial dilution from 1:500 to 1:64000 were performed using respective antisera. The antibody titer was determined by fixing a cut-off value which was obtained by the mean plus 3SD of the OD value of pre-immune serum. The highest dilution of the antiserum that showed an OD