Feng Li, Hao Qin, Xing Zhi, Wang Zhenfei and Wang Ziwei
the gap of sartorius in right limb. A large amount of pus flowed out, and the body temperature reached 39.5°C.
A total of 5 self-made double perfusion cannulas were cut into the required lengths and placed at the lowest point of the standing position and the lateral position, while rinsing with normal saline to keep low negative pressure suction. The drainage tube was replaced every 2 days.
On the second day, the patient’s body temperature returned to normal; on the seventh day, the scrotum and the sinus of the sartorius gap
I. Christiana, R. Aparnaa, R. Rohit, D. Nageswara Rao and P. Kaliraj
periodically after immunization and stored at -80 °C and the antibody titer was determined by ELISA, in which 96-well microtiter plates (Immulon2, Dynatech, VA, USA) were coated with 100 ng/well of respective antigen. The plates were blocked with 5 % skimmed milk, after washing with PBST and PBS, a 2 fold serial dilution from 1:500 to 1:64000 were performed using respective antisera. The antibody titer was determined by fixing a cut-off value which was obtained by the mean plus 3SD of the OD value of pre-immune serum. The highest dilution of the antiserum that showed an OD